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Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

Methods the bacteria identification and the antimicrobial susceptibility were assayed by automatic bacteria culture device microscan walkaway 40 from dade co.

采用美国dade公司的microscan walkaway 40全自动细菌鉴定及药敏分析系统进行细菌鉴定及药敏测定。

Medthods:Volatile oil of chive was extracted by means of water-vapour distillation and its chemical components were assayed by capillary gas thromat ography-mass spectrometry.

用水蒸气蒸馏法提取韭菜中韭菜的根茎、韭菜叶、韭菜花挥发油，并用毛细管气相色谱/质谱法，鉴定出其挥发油的化学成分。

Methods The expression of HSP-27 in human lenses was assayed using Weatern Blotting on 17 cases with old aged cataract and 7 normal human lenses.

方法采用免疫印迹法检测17例老年性白内障和7例正常人水溶性、水不溶性及SDS溶解的晶状体中的HSP-27含量。

By agar diffusion assay changes of nisin release from zein edible film with time and temperature were assayed.

采用琼脂扩散法研究了玉米朊复合薄膜中乳酸菌素在稀盐酸溶液中释放的活力随着时间和温度的变化。

Methods The expression of urokinase antigen on the pemphigus organ model was revealed by immunohistochemical method; PA activity was assayed in the acantholysis model; the influence of urokinase on the epidermis was observed by adding purified urokinase into the cultured skin explant.

方法应用免疫组织化学法检测天疱疮器官模型中尿激酶抗原的表达情况；发色底物法测定棘层松解模型中纤溶酶原激活剂的活性；在培养基中加入尿激酶，观察其对表皮的影响。

Preliminary insecticidal and acaricidal activities of the mentioned compounds were bio-assayed.

同样的，依维菌素B〓与羧酸在DMAP/DCC体系中直接酯化反应，得到10个5-IVB〓酯衍生物，产率66-82％。

Methods Serum containing achyranthes root was prepared, human mesenchymal stem cells were separated and amplified, MTT assay was used to access the effects of serum containing achyranthes root on human mesenchymal stem cells in vitro, alkaline phosphatase and osteocalcin were assayed to reflect the variations of stem cell differentiation, and the expression of osteogenic transcription factors with RT-PCR.

制备牛膝含药血清，分离与扩增人间充质干细胞，MTT法检测牛膝含药血清对体外培养的间充质干细胞增殖作用的影响，检测碱性磷酸酶与骨钙素以反映细胞分化水平的改变，RT-PCR检测成骨相关转录因子的表达。

Methods: Thyroid and adrenocortical functions were assayed by radioimmunoassay in 26 leukemia patients before and after BMT.

用放射免疫法测定了26例白血病患者用MAC方案预处理骨髓移植前、后的甲状腺及肾上腺皮质功能。

This study was aimed to evaluate the effect of benzyl alcohol on trehaloseloading red blood cells. The RBCs were incubated in 10, 30, 50 and 100 mmol/L concentrations of benzyl alcoholtrehaloe solution at 4℃ for 24 hours. The hemolysis rate of loaded RBCs was detected by using cyanohemoglobin kit, the intracellular trehalose level were assayed by sulfate anthrone method.

为研究苯甲醇对海藻糖负载红细胞的影响，在4℃条件下将红细胞孵育在浓度分别为10、30、50、100 mmol/L的苯甲醇海藻糖溶液中24小时，用氰化血红蛋白试剂盒测定海藻糖负载红细胞的溶血率，用硫酸蒽酮法检测红细胞内海藻糖浓度水平。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了