查询词典 assayed

Serum samples were then stored in 1.0 ml Eppendorf tube at -10C until assayed.Two hundred u 1 of thawed serum were piped into a 1.0 ml Eppendorf tube.

动物及给药方法体重在230g左右的Sprague Dawley清洁级雌性大鼠60 浙江大学2002年硕士学位论文只。

At last,the action spectrum properties of daidzein-phospholipid complex including UV spectrum,IR spectrum,differential scanning, and X-ray diffraction spectra were assayed.

在此最佳反应条件下，磷脂复合物结合率达94.2%。

After Ageratum - liquid, cisapride, and other singel berber was taken,the changes of gastrointestinal motility of rats were assayed.

结果：应用藿香正气液后大鼠胃肠运动显著增强，但各单味药的作用并不一致。

Amino acids of brain including glutamine,aspartic acid,almandine and Γ-aminobutyric acidwere assayed by amino acid analytic apparatus.

而在正常大鼠脑神经纤维网发育和髓鞘形成过程中，五羟色胺(5-HT)、苯丙氨酸的变化与很多脑疾病有关[1]。

The building rules of GLCM （ the best gray levels and pixels interval） were confirmed by experiment. GLCM texture parameters of 300 wood specimens were calculated and assayed, and then placed into a Competitive Neural Network for classification output. The results are as follows: 1） GLCM parameters Angular Second Moment contrast , correlation , entr

以"角二阶矩"、"对比度"、"相关"、"熵"、"方差"、"逆差矩"作为描述木材纹理的特征参数是合适的。2）在比例为1：1的512×512木材图像情况下，生成灰度共生矩阵的最佳像素间距为4，最佳图像灰度级数为128.3）木材纹理图像灰度共生矩阵的"角二阶矩"、"相关"和"熵"值最大的方向为纹理方向。4）竞争神经网络的分类正确率为88％。

According to recent researches, intracellular signalling pathway systems involved in appressorium differentiation is related with cAMP pathway and MAP pathway. In this research, 21 bacteria strains and Chaetomium cupreum strains were assayed for their antagonism to appressorium formation in M.grisea.

随着稻瘟病菌一系列致病基因的克隆及功能明确，附着胞形成的信号传导途径包括：cAMP途径和MAP途径，这些途径中基因的失活都影响附着胞的形成。

In the fourth part, I have principally assayed the opportunities and challenges that China probably will face in the course of peace rise, analyzed the arduousness and possibility of Chinese peace rise.

第四部分，主要分析中国和平崛起所面临的机遇和挑战，分析中国和平崛起的艰巨性与可能性。

He is assayed in the mind of his immediate superior, from the day he begins work, and after a time if he has merit, he is assayed in the council chamber of the firm.

在他的生活中就不可能从来都没有任何一丁点的机会，也不可能从来就没有碰到过一个极好的时机。事实上，任何一个人在他踏入工作岗位的第一天起就在他的顶头上司头脑中留下了一个印象，上司就会对他进行评价。

Methods Forty women and their babies (40) were enrolled in this study. Placental tissues were assayed for leptin mRNA by reverse transcription/polymerase chain reaction, and assayed for the obese gene protein leptin by Western-blot and immunohistochemistry. Blood was taken from the umbilical cord of the babies at delivery. Serum leptin was measured by radio-immunoassay. Neonatal anthropometric measurements were recorded within 48 hours after delivery.

方法在40例产妇分娩时采集胎盘与脐血，采用逆转录聚合酶链反应检测胎盘肥胖基因mRNA相对表达水平，采用Western-Blot检测胎盘肥胖基因蛋白表达水平，采用免疫组化观察胎盘肥胖基因蛋白表达位点，采用放射免疫法检测脐血瘦素水平，采用Ponderal指数［PI=100×体重/身长3］估测新生儿营养状态。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了