查询词典 assay

And Homo Sapien fetal brain cDNA Library was used to screen PRL-3-interacting proteins. 3. Identification and bioinformatical analysis of CDH22, a candidate PRL-3-interacting protein Interaction between PRL-3 and CDH22, a candidate PRL-3- interacting protein was identified by GST-pull down assay, co-immunoprecipitation assay and co-localization analysis in vivo and in vitro.

PRL-3相互作用蛋白的鉴定及其功能的生物信息学分析运用基于蛋白质水平的谷胱甘肽-S-转移酶融合蛋白沉淀、免疫共沉淀和共定位分析等技术对酵母双杂交系统初步筛选的结果进行体内和体外的验证。

Objective: To investigate the effects of sulindac on the growth and motility of ovarian cancer cell SKOV3 and its involved mechanism. Methods: In order to detect the effects of sulindac on cultured SKOV3, sulindac with different concentration and untreated control were set up. The changes in morphology of SKOV3 were observed by phase contrast microscopy. MTT colorimetric assay was used to study the effects of sulindac on SKOV3 cell growth. Motility of SKOV3 was determined by scarification assay.

目的：研究舒林酸对卵巢癌SKOV3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2（MMP2）、环氧合酶-2(COX-2)表达的影响：方法：将不同浓度的舒林酸加入培养液中观察其对SKOV3细胞形态的影响，采用MTT法观察舒林酸对SKOV3细胞生长的影响，通过划痕试验观察舒林酸对SKOV3细胞运动的影响，利用免疫细胞化学方法研究舒林酸对SKOV3细胞MMP2、COX-2表达的影响。

Methods The effects of various concentrations of tea polyphenols on proliferation, invasion and apoptosis of CGTH W-3 cells were observed by MTF assay, plate scarification assay and flow cytometer, respectively.

方法用MTT法检测细胞增殖活性，观察不同浓度茶多酚对CGTH W-3细胞增殖活性的影响；并通过培养板划痕法观察茶多酚对CGTH W-3细胞侵袭能力的影响；应用流式细胞仪检测各浓度茶多酚诱导CGTH W-3细胞凋亡的作用。

Methods: in order to detect the effects of sulindac on cultured skov3, sulindac with different concentration and untreated control were set up. the changes in morphology of skov3 were observed by phase contrast microscopy. mtt colorimetric assay was used to study the effects of sulindac on skov3 cell growth. motility of skov3 was determined by scarification assay.

目的：研究舒林酸对卵巢癌skov3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2（mmp2）、环氧合酶-2(cox-2)表达的影响：方法：将不同浓度的舒林酸加入培养液中观察其对skov3细胞形态的影响，采用mtt法观察舒林酸对skov3细胞生长的影响，通过划痕试验观察舒林酸对skov3细胞运动的影响，利用免疫细胞化学方法研究舒林酸对skov3细胞mmp2、cox-2表达的影响。

ABSTRACT A large passive-active neutron shuffler for the assay of drum containing uranium and transuranic waste is a nondestructive assay instrument which can work in passive and active mode.

大型桶装铀及超铀废物分类装置是一种非破坏性分析仪器，可工作于无源和有源两种方式。

Before repellency and anti-silverfish assay, the culture system for silverfish was established in this study. The best feeds for silverfish were cellulose and mixed feed in a 1:1 ration (the composition of mixed feed was milk powder: oatmeal: yeast= 1: 9: 9). Moreover, the hatching process was not necessary for the mature of silverfish eggs. According to the results obtained in the repellency assay, the essential oil of leaves and woods from C.

为了进行忌避和致死试验，本研究首先对衣鱼进行饲养及孵化条件测试，於实验后得知最适合衣鱼生长之条件为纸张纤维与混合饲料(酵母粉：麦皮：高蛋白＝9：9：1)混合比例为1：1时之生长状况最佳；另外，衣鱼卵并不需要成虫的孵化，而虫卵的生活条件则与成虫一致。

RESULTS: The best linearity of test was between 0.5-10 ng/mL. The intra-assay coefficient of variation was 8.17% and inter-assay CV, 9.72%.

结果：该方法最佳线性范围：ASP浓度0.5～10ng/mL，平均批内和批间变异系数分别为8.17%和9.72%。

To produce medicine serum of Liu-Shen-Wan with different dose, different time of pouring medicine, different time of blood sampling.2. To measure content of arsenic in serum with atomic absorption method, to qualitative analysis toadfish in medicine serum of Liu-Shen-Wan with thin layer chromatography.3. MTT and trypan blue assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells and to make certain the best scheme of making method of medicine serum of Liu-Shen-Wan.4. Gimsa dying assay observes morphology changes of apoptosis .In situ end-labeling method and flow cell machine quantificationally probes apoptosis phenomena.

1 分不同的给药剂量、给药天数和不同的采血时间制备含药血清。2 用原子吸收法测定血清砷含量；薄层色谱法定性检测六神丸含药血清中蟾酥成分。3 用MTT法、胎盘蓝染色法观察六神丸对HL-60细胞的增殖抑制作用，确定最佳血清制备方案。4 用吉姆萨染色法观察细胞凋亡的形态学变化，流式细胞仪、原位末端标记法定量检测细胞凋亡程度。

A variety of techniques from molecular biology have been used in research laboratories to study the effects of DNA binding small molecules, such as the gel mobility shift assay,~1H NMR, DNA foot-printing assay, and fluorescence-based assays, UV-Vis spectrum and viscometry.

目前有很多方法应用于实验室研究DNA与其他物质的相互作用，比如：凝胶电泳分析，核磁共振，DNA足印分析，荧光分析，紫外可见光谱分析以及黏度分析等。

A variety of techniques from molecular biology have been used in research laboratories to study the effects of DNA binding small molecules, such as the gel mobility shift assay,~1H NMR, electrochemistry analysis, DNA foot-printing assay, and fluorescence-based assays, UV-Vis spectrum and viscometry.

目前有很多方法应用于实验室研究DNA与其他物质的相互作用，比如：凝胶电泳分析，核磁共振，电化学分析、DNA足印分析，荧光分析，紫外可见光谱分析以及黏度分析等。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。