查询词典 assay

METHODS: A series of N-octyl-N'-succinyl chitosan labeled with fluorescein, were incubated with HepG2 liver cancer cells, K562 leukemia cells, A549 human lung cancer cells, and BGC cancer cells respectively and followed by using flow cytometry and enzyme-linked immunosorbent assay to assess the affinity and inhibition of N-octyl-N'-succinyl chitosan for the four kinds of tumor cells already mentioned.

用异硫氰酸荧光素对N-正辛基－N'-琥珀酰基壳聚糖进行标记，再分别与人肝癌细胞(HepG2)、人白血病细胞(K562)、人非小细胞肺癌细胞(A549)及人胃癌细胞共培养，通过流式细胞仪及酶联免疫检测仪测定N-正辛基－N'-琥珀酰基壳聚糖对肿瘤细胞的亲和性及抑制力。

Detection limits of the assay were calculated to be three times the baseline noise from drug free sample A steady-state bioconcentration and depuration of sulfamethazine (SM2) in the sturgeon A.

最后，利用流水式试验对SM_2在鲟鱼肌肉组织的生物富集和清除进行了研究。

Polyclonal antibody of Sulphamethoxazole,Sulfanilamide,Sulfadimethoxine were prepared, The establishment of dot-immunogold filtration assay for rapid dection of sulfamethoxazole residues would be a base for the use of DIGFA in dectecting residues of sulfanomides.

本研究制备了三种磺胺药的多克隆抗体，建立了快速检测磺胺甲噁唑的斑点免疫金渗滤法，为该技术在畜产品中磺胺药残留检测上的应用奠定了基础。

And cell differentiation was assayed by measuring the cellular alkaline phosphatase activity with dexamethasone as positive control. The influence of ethanol and dimethyl sulfoxide on cell proliferation and differentiation assay was also investigated.

成骨样细胞的分化采用测定细胞内碱性磷酸酶活性的方法，地塞米松为阳性对照药，同时考察了溶剂乙醇和二甲基亚砜对细胞增殖和分化的影响。

At passage 3, cells were induced by 3 mmol/L sodium butyrate and 0.1% dimethyl sulfoxide for 5 days. Cells were harvested for Western blotting assay.

细胞培养到第3代时，分别用3 mmol/L丁酸钠盐与0.1% DMSO进行诱导，5 d后收集细胞进行Western blotting实验。

In the second part of the thesis, we developed a very effective assay for the sulfotransferase that catalyzes the sulfation of serine and threonine. It utilized PAPS regenerated from 3-phosphoadenosine 5-phosphate by a recombinant PST using 4-methylumbelliferyl sulfate as the sulfuryl group donor. The change in the fluorescence intensity of 4-methylumbelliferone corresponded directly to the amount of active protein sulfotransferase.

第二部份则是探讨蛋白质亚硫酸转移酵素的活性检测方法，检测系统的主旨是用重组的PST催化受质4-methylumbelliferyl sulfate做为亚酸基提供者并转移至3'-phosphoadenosine 5'-phosphate再生PAPS ，此核苷酸的亚酸基再由蛋白质亚硫酸转移酵素催化，反应后所产生的4-methylumbelliferone之萤光变化可用来定量蛋白质亚硫酸转移酵素的活性。

As direct recordings for sound production in live specimens of this species could not easily be carried out, function of these swim bladder muscles was assessed by means of biochemical assay. Activities of Citrate synthase and L-lactate dehydrogenase, known to involve with energy supply during muscle contraction were assessed.

而直接测量活体的发音有执行上的困难，因此用酵素分析(包括Citrate synthase ，CS 与 L-lactate dehydrogenase ，LDH)来探讨此鱼鳔附属肌肉急其他不同类型肌肉之间的代谢模式，并用组织染色法测量肌纤维截面积可探究此肌肉之功能。

An indirect competitive enzyme-linked immunosorbent assay was established for detecting vitellogenin in the whole body homogenate of male swordtail fish.This technique was developed with antiserum of lipovitellinresistance as antibody and Vtg as antigen.

以卵黄脂磷蛋白(lipovitellin，Lv)抗血清为抗体，以纯化的卵黄蛋白原(vitellogenin，Vtg)为抗原，建立了间接酶联免疫吸附反应方法检测剑尾鱼雄鱼整体匀浆液中ρ。

METHORD: The chemical constituents of Schisandra micrantha were isolated by various column chromatographic methods. Their structures were assigned by spectroscopic methods including 2D NMR experiments, and their anti-HIV-1 activities and cytotoxicities were tested by microtiter syncytium formation infectivity assay.

采用多种柱层析分离进行化合物的分离和纯化，通过波谱分析鉴定化合物的结构，化合物的抗HIV-1活性通过对HIV-1感染C8166细胞致细胞病变的抑制试验得到。

Methods VEGF in the serum of 60 patients with RA and 28 healthy control subjects was quantified by our highly sensitive enzyme-linked immunoad sorbent assay, and its relationship to clinical and laboratory variables was analyzed . Synovial specimens were obtained from 1 RA and 1 OA patients undergoing synovectomy.

体内实验：用酶联免疫吸附方法检测了60例RA和28例健康对照血清中的VEGF值，并检测其他活动性指标及血清中特异性自身抗体：抗核周因子抗体、抗角蛋白抗体、抗环瓜氨酸肽抗体，分析它们之间的关系。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新