查询词典 assay

Methods The β-sitosterol and/or total protein of Pinella were incubated at different concentrations with cervical cancer cell line SiHa. The effects of β-sitosterol and/or total protein of Pinella on the viability of cells were tested by MTT assay.

在子宫颈癌细胞SiHa的培养基中加入不同浓度的β-谷甾醇和／或掌叶半夏总蛋白培养1~7d，采用MTT比色法检测细胞的活性。

Character: White or almost white crystalline powder, smell-less;Assay:≥98.5%;Solubility:Soluble in water, Slightly soluble in acetone and ethanol;IR:Complies with the working standard;Chemical identification:Red;Na Salt indentification:Complies with the standard;Relative substance:Complies with the working standard;pH:8.0-9.5;Loss on drying:≤0.5%; Heavy metal:≤ 20ppm

特征及特性：应为白色或类白色结晶性粉末；无臭，无味；含奥扎格雷钠(C13H11N2NaO2)≥98.5%；在水中溶解，在乙醇和丙酮中微溶；IR图谱与对照品一致；化学鉴别应显红色；溶液主斑点与对照品溶液主斑点的位置相同；钠盐鉴别符合规定；有关物质鉴别符合规定；pH：8.0～9.5；；干燥失重≤0.5%；重金属≤20ppm

Character: Yellowish or yellow crystalline powder,smell-less; Assay: 98.0%—102.0%; Specific Optical Rotation:+63.0°to +69.0°; Ph:3.0 to 5.0;Clarity of Solution complies with the standard. Related Substance:(Single impurities≤0.5%; total impurities≤1.5%);Methanol:≤0.3%;Dichlormethane≤0.06%Acetone≤0.5%;walter:7.0%—9.5%; Residue on Ignintion:≤0.1%; Heavy Metals:≤0.0002% Identification:Most absorb at the wavelength 220nm,256nm,370nm; The infrared spectrogram according to the comparison; positive reflection

特征及特性：淡黄色或黄色结晶性粉末，无臭；含量98.0%—102.0%；鉴别：1）在220nm、256nm、370nm的波长处有最大吸收；2）供试品溶液在主峰的保留时间与对照品溶液主峰的保留时间一致；3）供试品红外光吸收图谱与对照品的图谱一致；4）呈正反映；比旋度：+63.0°至+69.0°；酸度：3.0—5.0；溶液颜色符合规定；有关物质：（单个杂质≤0.5%；总杂质≤1.5%）甲醇：≤0.3%；二氯甲烷：≤0.06%；丙酮：≤0.5%；水分：7.0%—9.5%；炽灼残渣：≤0.1%；重金属：≤0.0002

The conventional calibration method and the standard addition method were compared in the assay of lead, cadmium, copper, and nickel in mineral Chinese medicines (borex, dragon teeth, oyster, smithsonite, and limonitium) by atomic absorption spectrometry.

传统中药大致可分为植物性、动物性及矿物性等三大类，本研究取自市售坊间常用种矿物性中药炉乾石、龙骨粉、牡蛎粉、硼砂、禹余粮等，以传统强酸氧化回流加热消化法。

Methods Cell number counting, MTT reduction assay, transmission electron microscope were performed in skin fibroblasts,and sodium arsenite was used as tester for inorganic arsenics.

方法通过直接细胞计数法和噻唑蓝还原法检测亚砷酸钠对皮肤成纤维细胞生长的影响。

Methods Cell titer 96 Non-radioactive Cell Proliferation Assay was used to study the growth of cultured thyrocytes from thyroid adenomas and paraadenomatous tissues in response to various concentration of sodium iodide.

手术切取甲状腺腺瘤及腺瘤旁正常组织，采用单层细胞培养技术和非同位素标记的细胞增殖分析法，检测不同浓度碘化钠经过不同作用时间对甲状腺细胞生长的影响。

The SMMC-7721 hepatoma cell line of experimental groups and control groups were cultured with the addition of sodium selenite, potassium iodide or triiodothyronine (T3). The cell quantity was checked by the MTT assay , and the cell cycle was observed by flow cytometry and levels of AFP and ALB in the culture solution were determined by RIA.The SD rats were divided into positive control group, negative control group, T3 group and mixture group (the mixture of sodium selenite, KI and T3). After two weeks of the administration of sodium selenite、 KI and T3,Walker256 cells were transplanted into the abdominal cavity. The administration was kept on for another two weeks, then observed the morbility and mortality of the rats with ascites carcinoma. At the end, the ascites were for the cells counting and the cell cycles analysising.

方 法：在体外，应用细胞培养的方法，培养SMMC－7721肝癌细胞系，分为试验组和对照组，试验组加入不同浓度Se、I或T3干预，培养一段时间后，通过噻唑蓝试验、流式细胞仪，观察细胞数量及细胞周期的变化，测定细胞培养上清液中甲胎蛋白、白蛋白的水平，观察细胞分化情况；在体内，将试验用SD大鼠分成阴性对照组、阳性对照组、T3组和混合组（为Se、I和T3混合物），首先预防性用药2周，然后给试验组大鼠和阳性对照组大鼠腹腔注射Walker256细胞，制造腹水癌大鼠模型；继续用药2周，观察各组大鼠发病率及死亡率，测量不同时期大鼠血清FT3含量，通过流式细胞仪测量大鼠腹水细胞数及细胞周期变化。

Weeks later, all the rats were killed, the levels of sodium pump α1-,α2-, and α3-subunit mRNA and protein in the cortex of kidney were detected with reverse transcription polymerase chain reaction method and immunohistochemical assay, respectively.

分别应用RT-PCR及免疫组化技术，探讨1k1c高血压大鼠肾脏皮质钠泵α1、α2及α3亚单位mRNA及蛋白水平基因表达的改变。

Weeks later, all the rats were killed and sodium pump α1-,α2-, and α3-subunit in aortic smooth muscles were detected with reverse transcription p ol ymerase chain reaction method and immunohistochemical assay at both mR NA and protein levels, respectively.

分别应用分子生物学 RT-PCR及免疫组化技术，探讨1k1c高血压大鼠动脉平滑肌细胞钠泵α1、α2及α3亚单位mR NA及蛋白水平基因表达的改变。

This assay was applied to determination of HRP in the solution of hepatitis B surface antigen labeling HRP, with satisfactory results.

该法应用于酶联免疫乙肝试剂盒中辣根过氧化物酶活力的测定，结果比较满意。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。