查询词典 assay

Results By optimization test, capture antibody and detection antibody (from PVA detection kit, Agdia, USA) were finally diluted at 1:50 (4ug/mL) and 1:25 to be used in one step CBA, respectively. A total CBA reaction time of 2 h was needed. No cross reactivity with other plant virus similar to potato virus Y, lettuce mosaic virus and tomato ring spot virus were found. Detection sensitivity for positive control sample in one-step CBA was 4 folds higher than that in normal plate double antibodies sandwich enzyme-linked immunosorbent assay.

通过实验优化检测条件，最佳捕获抗体工作浓度为4μg/mL、最佳检测抗体工作浓度为1：25倍稀释、最佳反应时间为2h；与马铃薯Y病毒、莴苣花叶病毒、番茄环斑病毒等均未出现交叉反应；阳性样品经64倍稀释后依然可检出，检测灵敏度是传统微孔板ELISA的4倍。

The in vitro anticancer activities of the ruthenium complexes were screened by MTT assay.

采用MTT法评价了3种异构体对多种肿瘤细胞株的体外抗肿瘤活性以及对正常细胞的毒性。

A gold immunostrip for rapid detection of Chloramphenicol was developed based on competitive immunochromatographic assay. And the mixtures of saccharose, dextran, polyvinylpyrrolidone, polyethylene glycol, bovine serum albumin, tween20 and antiseptic, etc, as protectants were designed and prepared. Different part of the strip was treated with the protectants, and then thermo-stability of the strip was investigated with storage at 4℃, 37℃ and 60℃.

根据竞争性金免疫层析法原理，研制了氯霉素速测金标试纸条，并选择了蔗糖、葡聚糖、聚乙烯吡咯烷酮、聚乙二醇、牛血清蛋白、表面活性剂Tween20、防腐剂等物质，采用经验尝试法配制了一系列的配方，对试纸条不同部位进行处理，通过37℃和60℃下热稳定性测试，并与未经稳定剂处理的对照进行比较，筛选出适宜的试纸条保护剂。

The sensitivity and specificity of the assay were evaluated using 7 saprophytic serovars, 37 pathogenic serovars, and 15 other microorganisms.

用7种腐生性血清型，37种致病性血清型以及15种其他微生物评估了本法的特异性和灵敏度。

METHODS: In the rat cardiac hypertrophy model produced by constriction of the abdominal aorta, urotensin II binding sites in myocardial sarcolemma were determined with radioligand assay first day and 30th day after operation.

在腹主动脉缩窄复制的大鼠压力超荷性心肌肥大模型上，采用放射性配基结合技术，测定术后1 d和30 d大鼠心肌浆膜上UII受体的结合位点。

Objective To compare the diagnostic effect of ScAg assay,collective egg incubation and Kato-Katz methods so as to provide effective diagnostic method after periodic mass treatment.

目的观察比较血吸虫循环抗原与病原学诊断方法的应用效果，为经过周期性群体化疗后的血防工作提供有价值的诊断方法。

ScAg assay with standard agent,egg incubation and Kato-Katz methods were conducted and compared in parallel.

结果以粪便检查阳性的血吸虫病患者例数为金标准，ScAg的阳性检出率为95.2%(119/125)，集卵孵化法阳性率为81.6%(102/125)，Kato-Katz法阳性率为65.6%，(82/125)。

Result The positive rates were 95.2%(119/125)of ScAg assay,81.6%(102/125) of egg incubation method,and...

粪检总阳性中集卵孵化法漏检率为18.4%(23/125)，Kato-Katz法漏检率为34.4%(43/125)。

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h，MTT法检测ox-LDL对RAW264.7细胞生长的影响；流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡；用特异性siRNA沉默Daxx在RAW264.7细胞中的表达，通过AO／EB染色观察基因沉默后的细胞凋亡形态学改变；高效液相色谱检测细胞内胆固醇含量；油红O染色观察细胞内脂滴的形成情况；Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况；用Western Blot印迹法检测caveolin-1蛋白的表达。

Methods Morphologic change of cells were observed with fluorescence microscope;MTT assay was used to evaluate survival rates Survival rates of GLC-82 cells;The influence of NA to cell migration was analyzed by scarification test; The percentage changes of apoptosis and the distributional percentage of cell cycle were analyzed with flowcytometry.

荧光显微镜观察细胞形态的变化；MTT 法检测不同浓度烟酰胺培养细胞后细胞的存活率；划痕试验检测不同浓度的烟酰胺对细胞愈合力的影响；流式细胞仪检测细胞周期和凋亡率。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。