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To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

The expression of RG could be detected in CEF by indirect immunofluorescence assay and Western blot analysis.

该病毒在鸡胚成纤维细胞中繁殖，在人源细胞如TK143中几乎不增殖。

When applied in ovule culture media, UDP-rhamnose, precursor of pectic polymer, was able to stimulate significant fiber elongation, while rhamnose was inactive in the same growth assay.

在胚珠体外培养的培养液中添加UDP-鼠李糖能显著速进纤维的伸长，而加入鼠李糖对纤维伸长没有影响。

Methods ELISA was used to assay anti-CCP antibodies in 30 patients with RA, 35 patients with other rheumatic disease and 20 normal controls respectively.

用 ELISA方法检测 30例 RA，35例其他风湿性疾病， 20例非风湿性疾病中的抗 CCP抗体的分布。

Objective To develop the assay methods of riboflavin in riboflavin sodium phosphate.

目的 建立核黄素磷酸钠中核黄素的含量测定方法。

Objective: To develop a ribonuclease protection assay kit for quantitative analysis of mRNA expression in the tissues and cells.

目的：开发核糖核酸酶保护分析试剂盒，用于组织和细胞mRNA定量分析。

Initial alternatie analysis of RNA was performed by ribonuclease protection assay.

在核糖核酸酶保护试验中对RNA用初始的交替式分析法。

The applicationof ribonuclease protection assay was discussed in the identification of CMV strains.

讨论了核酸酶保护法在CMV株系鉴定中的作用。

Ribonuclease protection assay ; kit ; quality control

核糖核酸酶保护分析；试剂盒；质量检测

Among these techniques, Northern blot, ribonuclease protection assay, real-time quantitative polymerase chain reaction, which are based on differentially screening and hybridization technology, have been applied most frequently.

本文概述了用于检测mRNA丰度的常规分析方法，即Northern印迹、RNase保护试验和实时定量PCR技术，特别是为对数量不多的基因进行表达情况的测定时提供了较为实用的方法。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。