查询词典 assay

METHODS: Semiquantitative RTPCR assay was used to detect the expression of MICA mRNA and SP immunohistochemistry to detect the expression of MICA protein in 41 patients with gastric cancer. Spearman rank correlation was used to analyze the correlation between them.

采用RTPCR技术检测41例高原地区胃癌组织和相应的癌旁粘膜组织中MICA mRNA表达，采用免疫组化SP法检测MICA蛋白在胃癌和癌旁组织中的表达，Spearman相关关系检验分析它们之间的相关性。

Objective: To investigate the effect of tanshinone ⅡA on HL-60 and K562 cells apoptosis,and to assay the inhibition of the telome rase activities in the leukemia cell apoptosis induced by Tanshinone.

中文摘要：目的观察丹参酮ⅡA对HL60，K562细胞端粒酶活性的抑制作用和对凋亡相关基因的影响，探讨丹参酮ⅡA对造血细胞的作用机理。

Methods Samples of 12 patients with RAU active phase were studied by it suppressor assay.

应用刀豆碱(concanavalin A， ConA)激活产生的淋巴细胞抑制检测法探查了12例溃疡期患者外周血淋巴细胞的抑制活性。

RT-PCR assay revealed that realgar nanometer suspension reduced HPV16E6/E7 gene expression.

RT-PCR检测显示随着纳米雄黄混悬液作用浓度的增加，HPV16E6和E7的表达不同程度地受到抑制。

NO was determined by nitrate reductase method. Intracellular oxidant stress was measured by flow cytometric assay.

NO的测定应用硝酸还原酶法，细胞内呼吸爆发的检测用流式细胞术。

MAIN OUTCOME MEASURES: Cell surface antigen expression. MTT assay was used to detect cell activity. Flow cytometry was utilized to measure DNA content, protein content and reproductive cycle.

主要观察指标：细胞表面抗原的表达，MTT法检测细胞活性，流式细胞仪测定细胞DNA含量、蛋白质含量和增殖周期。

Citri had been selected by ribosome display in previous research. In order to express the scFvs with bio-activity efficiently, the genes of the selected scFvs were transformed into Escherichia coli HB2151 for expressing resoluble antibodies. The expression levels of scFvs were detected by dot blotting and Western blotting, and the expressed scFvs were purified by affinity chromatography, and then the activity of the purified scFvs was tested by enzyme-linked immunosorbent assay .

Citri， Xac高亲和力单链抗体(Single chain variable fragment， scFv)GX13、GX44和GX95，为高效表达具有生物活性的单链抗体，本研究将前期筛选的高亲和力单链抗体基因转入大肠杆菌HB2151中进行可溶性表达，点印迹和Western印迹检测可溶性单链抗体的表达水平，亲和层析纯化表达的单链抗体，酶联免疫吸附法检测纯化单链抗体的抗原结合活性。

Collagenase and 25 m filtration web, and characterized in terms of the expression of different phenotypic markers of osteoclasts and their ability to resorb bone matrix. The phenotypic markers of DCs include the expression of tartrate-resistant acid phosphatase, acid phosphatase, resorption pit assay, reaction to calcitonin and identification of F-actin.

于37oC，5％CO。培养箱中培养1－2小时后，弃培养液：lxPBS清洗数次；0.25％胰酶－EOTA 37℃消化5分钟，加入含川％FBS的a－MEM，经小25tim过滤网过滤；IX PBS清洗末过滤细胞，即得到纯化的破骨样细胞，并进行细胞形态学和分于生物学鉴定。

The result of enzyme assay revealed that polygalacturonaseand polygalacturonate lyase play key role in the retting process.

酶活性测定显示PGase和PAL在构皮浸解过程中起关键作用。

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。