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Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

By using radioactive ligand receptor binding assay and observing animal activities, this paper reports the effects of convulsants of picrotoxin, penicillin and kainic acid on the 3H GABA A binding in the cerebral cortex, hippocampus and cerebellum of mice.

采用放射配体受体结合分析法和动物行为观察，研究了致惊药印防己毒素(picrotoxin ，Pic)、青霉素(Penicillin ，PG)、红藻氨酸(kainicacid ，KA)对小白鼠大脑皮层、海马、小脑三脑区3 H GABA与GABAA 受体结合的影响。

Ointment extracted from Plastrum Testudinis was separated by silica gel column chromatography, gradiently eluted by petroleum ether-ethyl acetate, and 16 (Ts-1～Ts-16) components were obtained, which were biologically evaluated by MTT assay and flow cytometry on the proliferation of rat marrow-derived mesenchymal stem cells. Results showed that Ts-12 induced proliferation compared with the control group ( p ＜0.05), while Ts-4 showed inhibitive effect. Other groups showed no significance ( p ＞0.05). GC-MS was used to analyze the chemical components in different groups.

中药龟板提取物浸膏经硅胶柱层析，用石油醚-乙酸乙酯作洗脱剂，梯度洗脱，得到16个组分；采用MTT法和流式细胞技术研究了它们对鼠骨髓间充质干细胞的增殖作用，实验结果显示组分Ts-12具有促进rMSCs增殖的作用( p ＜0.05)，而组分Ts-4则表现出抑制rMSCs增殖的作用( p ＜0.05)，其它样品对rMSCs的增殖作用不显著( p ＞0.05)，不具统计学意义。

objectiveto predict the promoting effect of chinese medicine on proliferation of rat bone marrow mesenchymal stem cells by bioactivity fingerprint in vitro.methodsthe ointment of plastrum testudinis was extracted by petroleum ether,ether and dichloromethane in burn to gain the extracts.the effects on the proliferation of bmmscs were examined by mtt assay and flow cytometry analysis, and each sample was studied by gc-ms.

目的运用生物活性指纹图谱预测其它中药能否促进骨髓间充质干细胞（bone marrow mesenchymal stem cells，bmmscs）。方法龟版浸膏依次用石油醚、乙醚、二氯甲烷提取，得到溶剂提取物，用mtt 比色法及流式细胞仪器研究了溶剂提取物干细胞活性，采用gc-ms 技术研究每一个提取物，根据总离子流色谱图的相同保留时间和相应峰面积，获得龟版浸膏 gc-ms 生物活性指纹图谱。

Development of an Indirect Enzyme-Linked lmmunosorbent Assay for Seromonitoring Contagious Bovine Pleuropneumonia Using Recombinant Lipoprotein LppQ of Mycoplasma mycoides subsp mycoides SC as Antigen.

建立一种以霉菌样支原体亚种重组脂蛋白 LPPQ 为抗原的间接 ELISA 方法，能够对牛传染性胸膜肺炎进行血清学检测

Methods The subjects were divided into two groups: PIH group (n= 15) and normotensive control group (n=15). Peripheral lymphocytes of the subjucts were separated by Ficoll-Hypaque density centrifugation. The lymphocytes were cultured in RPMI-1640 medium with or without pokeweed mitogen for ten days.Then the supernatant was collected and the IgG and IgM were measured by enzyme linked immunosorbent assay.

采用密度梯度离心法分离妊高征组(15例)和正常妊娠组(15例)的外周血B淋巴细胞，并在有或无美洲商陆有丝分裂原存在的情况下培养10天后，收集上清液，采用酶联免疫吸附试验测定每1×106个B淋巴细胞所分泌的IgG和IgM的量。

As the As is concerned, adsorptive stripping voltammetric using DDC can get reproducible results in low concentration range, and polarograph can be used to assay high content.

对于As(上标 3+)，低浓度时用DDC的吸附溶出法可获得较好的重现性，当浓度高时，可考虑极谱法。

MethodsMTT assay was used to examine the effect of Portulaca oleracea L. on the proliferation of HepG-2 cell and flow cytometry was used for the cell cycle distribution.

采用MTT比色法观察马齿苋提取物抑制肝癌HepG-2细胞增殖；采用流式细胞仪检测马齿苋对HepG-2细胞周期分布的影响。

Objective To investigate the inhibition of Portulaca oleracea L. on proliferation hepatoma HepG-2 cells.MethodsMTT assay was used to examine the effect of Portulaca oleracea L.

方法采用MTT比色法观察马齿苋提取物抑制肝癌HepG-2细胞增殖；采用流式细胞仪检测马齿苋对HepG-2细胞周期分布的影响。

On proliferation hepatoma HepG-2 cells.MethodsMTT assay was used to examine the effect of Portulaca oleracea L. on the proliferation of HepG-2 cell and flow cytometry was used for the cell cycle distribution.

方法采用MTT比色法观察马齿苋提取物抑制肝癌HepG-2细胞增殖；采用流式细胞仪检测马齿苋对HepG-2细胞周期分布的影响。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。