查询词典 assay

Methods:Phenobarbital and Valproic acid were added into astrocytes and cultured permanently for 30 days. P-glycoprotein, a product of MDR1, was detected with immunocyto chemistry. Then, 5μg/ml Sibelium was added to resistance cells and nonresistance cells, MTT assay was used to measure reverse multiple and relative reverse efficiency rate.

将苯巴比妥钠和丙戊酸钠分别加入培养的星形胶质细胞内，30天后用免疫细胞化学法检测MDR1的表达产物P-糖蛋白，继之将终浓度为5μg/ml的西比灵分别加入培养的正常细胞及MDR1表达增强的细胞内，用四甲基偶氮唑盐法计算逆转倍数和相对逆转效率，了解西比灵逆转MDR1表达的作用。

Results: Of 59 superficial bladder cancer samples, sensitivity tests were successfully performed in 45 samples, and the others failed, the successful rate was 76.3%.Diversity of inhibiting rates of antitumor drugs was observed in samples from different individuals. Except MTX in stage of G1 and G3, no correlation has been found between the phenotype of sensitivity assay and the pathological grade and stage. Sensitivity and mean inhibiting rates of antitumor drugs in the primary group were notedly higher than that in the recurrent group.The combination of two therapy agents, which have different mechanisms or different cell-cycle targets, showed significantly more sensitivity and higer mean inhibiting rates than single one in superficial bladder cancer in different individuals.

结果：14例失败，45例获得成功，总体可评价率为76.3％，不同抗癌药物对不同个体膀胱癌的抑制率存在明显差异；除MTX对膀胱癌G1和G3级平均抑制率差异有统计学意义外，其它抗癌药物对不同个体膀胱癌细胞的平均抑制率与膀胱癌的病理分级、分期均无关；抗癌药物对初发膀胱癌的敏感率和平均抑制率均高于复发膀胱癌；两种不同作用机制的抗癌药物联合应用对不同个体膀胱癌细胞的敏感率和平均抑制率均高于单一药物，二者敏感率和平均抑制率差异有统计学意义。

Background and Objective To establish an international standard for nucleic acid amplification technology assay for HCV RNA in China.

背景和目的丙型肝炎病毒核酸检测缺乏统一的定量标准，给临床的诊断和治疗带来了很多问题。

The nucleoid size analysis, the comet assay and quantitative polymerase chain reaction.

细胞核大小分析，慧星检测法，PCR定量法。

METHODS:Nitrite was determined based on Griess reaction. iNOS mRNA was analyzed using Northern blot. NFκB in the cell nucleole was detected with electrophoretic mobility shift assay.

摘 要］目的：探讨NFκB在大鼠肺微血管内皮细胞iNOS基因诱导表达中的作用及抗氧化剂对内皮细胞iNOS诱导表达的影响及其机制。

METHODS:Nitrite was determined based on Griess reaction. iNOS mRNA was analyzed using Northern blot. NF κ B in the cell nucleole was detected with electrophoretic mobility shift assay.

Griess法测定细胞培养上清液NO-2水平以反映NO的生成，Northern印迹分析iNOS mRNA水平，EMSA法测定细胞核内NFκB的结合活性。

Abstract］ AIM:To investigate the role of nuclear factor κB in the induction of iNOS gene by TNFα and LPS in endothelial cells and the effect of antioxidant on the induction of iNOS. METHODS:Nitrite was determined based on Griess reaction. iNOS mRNA was analyzed using Northern blot. NFκB in the cell nucleole was detected with electrophoretic mobility shift assay.RESULTS:(1)NO production and iNOS mRNA expression induced by LPS and TNFα was blocked by pyrrolidine dithiocarbamate or N-acetylcysteine.

研究表明内皮细胞激活时iNOS表达的调控主要发生在转录水平。iNOS基因的启动子区域含有多个转录因子的结合位点包括NFκB、AP-1、NF-IL6，Oct-1等［2］，而核因子κB(nuclear factor κB， NFκB)被认为在多种参与炎症反应的蛋白质因子的基因调控中担任重要角色，因而可能是内皮细胞激活的调控中一个重要转录激活因子［2，3］。

The third part of this project is to evaluate the safety of the nutria gland. The assay items will include single dose acute toxicity, multiple dose subacute toxicity and mutagenicity test.

在安全性评估方面，本计画将进行北美巨水鼠腺体之动物毒性试验，含单一剂量急毒性及重覆剂量亚急毒性，以及基因毒性试验。

Both cylinder plate assay and MIC test demonstrate that ginger oleoresin is of obvious inhibition activity.

结果表明，生姜油树脂对试验中几种测试菌均有不同程度的抑制作用，尤其对真菌的抑制作用明显强于食品常用防腐剂山梨酸钾。

Methods: The effects of Opuntia on proliferation of fibroblast were measured with MTT colorimetric assay, and PCNA productions were examined with immunocytechemistry.

以小鼠成纤维细胞株(NIH3T3)为研究对象，采用四氮唑盐比色法测定仙人掌对成纤维细胞增生的影响，采用免疫细胞化学染色法检测成纤维细胞的增殖细胞核抗原的表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。