查询词典 assay

We also analyze the function of the MCP gene in drop assay.

利用趋化分析方法初步分析了此基因在趋化中的作用。

Melanocytes were cultured with H2O2, the proliferation and melanogenesis of melanocyte were analysed by MTT and NaOH assay.

培养的黑素细胞中加入H2O2，用MTT法检测黑素细胞增殖，NaOH法检测黑素合成。

Methods MTT assay was used to evaluate the inhibitory activities of melatonin on HeLa cells.

目的 探讨褪黑素在抑制HeLa细胞增殖剂量下对抗氧化作用的影响。

Methods The cells were divided into 5 treatment groups（10,25,50,75 and 100 μmol·L-1 QUE）, blank control and menstruum control group. The rat C6 cells were cultivated to 1×106·mL-1 in the RPMI 1640 medium, then added into 96 holes board with various doses of QUE by 3 holes per group,and MTT assay was used to observe the proliferation of the cells treated for 24,48 and 72 h. The change of cell cycle was also observed by flow cytometry after the cells were treated with 50 and 100 μmol·L-1 QUE for 48 h. The changes of the protein P53 and Bcl-2 of C6 cells treated with 50 μmol·L-1 QUE for 48 h were detected by immunocytochemical methods.

按QUE浓度分成10、25、50、75及100 μmol·L-15个处理组和空白对照组及溶剂对照组，大鼠脑胶质瘤C6细胞在RPMI 1640培养基中生长达1×106·mL-1后，在96孔板中分别加入上述浓度的QUE继续培养，每组设3复孔，作用24、48及72 h，采用MTT比色法检测QUE对大鼠脑胶质瘤C6细胞的增殖抑制情况，流式细胞术对50及100 μmol·L-1的QUE作用48 h的大鼠脑胶质瘤C6细胞进行周期分析，免疫组化法检测50 μmol·L-1的QUE作用48 h 的p53和bcl-2基因产物。

After immunization, kinetics of antibody response, isotype of IgG antibody, and antibody responses to the individual components of the chimeric antigen were detected by ELISA. Interactions of the antibodies with native antigens located on surface of merozoite of Plasmodium falciparum were analyzed by indirect immunofluorescent assay.

以ELISA检测免疫血清中特异性抗体的动态变化、IgG抗体亚型及对融合抗原各组分的抗体水平，以间接荧光抗体实验分析免疫血清对恶性疟原虫天然抗原的识别情况。

While erthrocyte, hemoglobin and Hela cell were under oxidative damage induced by the hydrogen peroxide (H2O2) or tert-butyl hydroperoxide on different concentrations of dihydromyricetin, hemolysis rate of erythrocyte and methemoglobin converted from hemoglobin was estimated by ultraviolet-visible absorption spectroscopy. Whereas mmalondialdehyde from erythrocyte was measured by thiobarbituric acid colorimetry, the cellular viability of Hela cell was examined using MTT 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide assay.

在不同的二氢杨梅素浓度下，用过氧化氢或过氧化特丁烷对红细胞、血红蛋白和Hela细胞进行氧化伤害，以紫外－可见光分光光度法检测红细胞溶血率和由正常血红蛋白生成的高铁血红蛋白，硫代巴比妥酸法测定红细胞中的丙二醛，MTT法检测Hela细胞的活力。

Objective: To establish a reporter gene-based assay for identification of estrogenic compounds in MCF7 cells and study the estrogenic effects of 2,4-DDT and methoxychlor.

目的：建立基於报告基因的MCF7细胞体外雌激素样物质检测方法，并研究2，2-双-1，1，1-三氯乙烷(2，4-DDT)和甲氧氯的雌激素样作用。

All results from morphological, physiological and biochemical characterization, the 16S rDNA sequence and phylogenetic assay identified that the strain belongs to Micrococcus luteus. The DNA fragment encoding mature peptide of fibrinolytic enzyme was cloned by PCR and sequenced.

利用传统细菌分类鉴定方法对其形态和生理生化特征进行了研究，发现其与藤黄微球菌的特征相符， 16S rDNA序列分析的结果也表明它与藤黄微球菌的16S rDNA序列的同源性高达99%，因此该菌在系统分类学上属于微球菌属藤黄微球菌。

Only 1 microliter of serum and 3 hours were needed for Luminex assay.

检测CEA、AFP、HBsAg的灵敏度分别为96.4％、95.8％、92％，特异度分别为95.6％、94.2％、100％，准确度分别为96％、95％、96％。

MPC is a method of simplicity, rapidity and sensitivity for protein quantification assay especially suitable for the determination of large number of microliter samples.

考马斯亮蓝微盘比色法是一种简单、快速、灵敏的蛋白质测定方法，尤其适用于大批量、微量样品的蛋白质含量测定。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。