查询词典 assay

In this paper, the ability and mechanism of antibacterial and immune defense ofChinese shrimp was studied using cytological, biochemical, molecular biological andproteomic methods. A sensitive and stable antibacterial and lysozyme assay methodswere selected. The effect of Vibrio anguillarum and laminarin (a kind of beta-1,3-glucan) on the immune ability of haemolymph of Chinese shrimp was analyzed.The changes of total haemocyte counts, antibacterial ability, lysozyme activity andlysosome membrane stability were detected.

本文采用细胞学，生物化学，分子生物学和蛋白质组学等技术，对对虾的抗菌能力和免疫防御机制进行研究，筛选了灵敏稳定的抑菌和溶菌检测方法；并对灭活鳗弧菌和海带多糖(laminarin，一种B-1， 3 葡聚糖)免疫诱导后的中国明对虾血细胞数目，血淋巴的抑菌活力，溶菌能力，溶酶体膜的稳定性进行分析；对两种重要免疫因子溶菌酶和精氨酸激酶进行克隆和表达分析。

Since respiratory tract infections, including IB, are critical in the poultry industry, a multiplex reverse transcription-polymerase chain reaction assay that detects avian viral respiratory pathogens in the same reaction was developed for the economic and clinical needs. Four sets of specific oligonucleotide primers for infectious laryngotracheitis virus, IBV, Newcastle disease virus, and avian influenza virus were used in this study.

由於呼吸道疾病对於家禽产业有很大的影响，因此基於经济与应用在临床上区别诊断的考量，本实验室发展多引子反转录聚合酶链锁反应，一共使用四组针对传染性喉头气管炎病毒、传染性支气管炎病毒、新城病病毒、以及家禽流行性感冒病毒的特异性引子来进行测试。

Objective:To develop an assay for the determination of phosphorus level in soya lecithin by spectrophotometry.

提要 目的：研究分光光度法测定大豆磷脂中磷的含量。

It was demonstrated that the cloned plcB gene appeared to be 834 bp in length, entirely identical with that published in GenBank The fusion protein GST-tagged broad- range phospholipase C showed bioactivity and immunogenicity in lecithinase test and Western blotting assay respectively.

结果 克隆的plcB基因长834bp，与GenBank上公布的序列完全相同；表达的与GST标签蛋白融合的广谱磷脂酶C蛋白具有磷脂酶生物活性和免疫原性。

To explore the influence of reticuloendotheliosis virus and subgroup J avian leucosis virus infection on chicken cellular immunity,the mitogenic response to ConA of blood and spleen lymphocytes tested and analyzed with 3H-thymidine(3H-TdR) incorporation assay at different intervals after infection or co-infection of single or both viruses.

自1974年分离出网状内皮增生症病毒[1]和1991年分离出禽白血病病毒J亚群[2]以来，在我国先后分离出不同毒株的REV和ALV-J，并开展了病原学、流行病学、致病性和免疫反应以及预防诊断等方面的研究，揭示出不同的致病性，并且确认它们都可造成感染鸡群免疫抑制，成为一些

Objective To establish an assay method for whole blood leukocyte phagocytosis in decrease of test time of clinical samples.

目的 建立一个同时测定多个样品的全血白细胞吞噬功能测定的方法，缩短临床标本检测的时间。

objective:to apply the kinetic turbidimetric limulus test in the endotoxin assay of shengmai injection.

摘 要 目的：应用动态比浊法鲎试验定量测定生脉注射液中的细菌内毒素。

Objective:The kinetic turbidimetric Limulus test was used in the endotoxin assay of Ligustrazin Hydrochloride injection.

目的：应用动态比浊法鲎试验定量测定盐酸川芎嗪注射液中的细菌内毒素含量。

In this study, we found out that linalool, a small-molecule compound, could inhibit the growth of various human lymphocyte leukemia cell lines significantly. Meanwhile, it showed little toxicity to normal hematopoietic cells. To determine effects of linalool on the growth of a serious of human leukemia cell lines and normal hematopoietic cells and to explore its possible mechanisms, we employed various technologies, including MTT assay, Flow cytometry, Human Cancer PathwayFinder II Gene Array, Western Blot, RT-PCR and so on.

我们在筛选抗肿瘤小分子化合物时，发现天然小分子化合物芳樟醇能明显抑制多种人淋巴细胞白血病细胞增殖，而对正常人骨髓造血细胞及外周血细胞的增殖没有明显的影响，为此我们用MTT技术、流式细胞术、肿瘤相关信号通路分析技术以及Western blot、RT-PCR等手段和方法探讨了芳樟醇对不同类型白血病细胞系及相应正常造血细胞中的影响情况，及其诱导白血病细胞凋亡的可能分子机制。

Results showed that the standard curve made by pMD-18T-GHR had good linear dependence, and the fluorescent quantitative RT-PCR assay was sensitive, specific and reliable.

结果表明，由pMD-18T-GHR所构建的标准曲线线性关系良好，建立的GHR基因荧光定量PCR检测方法灵敏度高、特异性强（可以检测出低于10个拷贝/μl的样品），准确可靠。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。