查询词典 assay

Objective: Evaluate the performance characteristics of Human Chorionic Gonadotropin strips by Colloidal Gold Immunochromatographic Assay.

目的：评价人绒毛膜促性腺激素测试条的分析性能。

Results: The linear range of gramicidin was 0.02~0.06U/mL, The linear range of tyrothricin was 0.1~0.3 U/ml, The average recovery rate of the standard curve assay was 101.7%(n=15, RSD=3.4%).

结果：短杆菌肽线性浓度为0.02~0.06U/mL，短杆菌素线性浓度为0.1~0.3U/mL，标准曲线法的平均回收率为101.7%(n=15， RSD=3.4%)。

Methods: The effect of MXC on proliferation and apoptosis of granulose cells was assayed by using MTT assay to measure the cell vitality, flow cytometer to detect cell apoptosis, electron microscopy to observe the morphological changes after the action of MXC.

MTT法测定卵巢颗粒细胞在MXC作用后的细胞活力；流式细胞技术检测细胞凋亡变化；电镜观察细胞的形态学改变等；综合评价MXC对卵巢颗粒细胞增殖和凋亡的影响。

Vascularlevels of guanosine 3', 5'-cyclic monophosphate were measuredwith enzyme-linked immunosorbent assay.

用酶联免疫吸附剂测量血管水平的鸟嘌呤3，5-环一磷酸。

It has been shown by haemolytic plaque-forming cell assay that there is an immunosuppressive factor with high activity in the cell-free aseitic fluid of Sarcoma 130 maintained in 615 strain mice.

用溶血空斑试验证明，在615小鼠上移植的肉瘤180的无细胞腹水液中存在着高活性的免疫抑制因子。

The study was purposed to investigate the effect of extract of Agkistrodon Halys venom on proliferation and apoptosis of K562 cells. The inhibition of K562 cell proliferation was measured by MTT assay; The morphologic changes of K562 cells was observed by microscopy; the apoptosis of K562 cells was measured by flow cytometry; the activity of extracellular signal-regulated kinase in K562 cells was detected by Western blot.

本研究探讨皖南蝮蛇毒蛋白提取物对白血病细胞K562增殖的影响及诱导凋亡的作用，采用MTT法检测不同浓度的蛇毒蛋白提取物对K562细胞生长的影响；应用电子显微镜观察药物作用后K562细胞形态的改变；流式细胞术检测蛋白提取物对K562细胞细胞凋亡作用；Western blot检测细胞增殖酶活性的变化。

It has good specificity and sensitivity by a competitive inhibition enzyme-linked immunosorbent assay. The hapten was linked with enzyme and determination method was established primarily that based on competitive ELISA. The limit of this method was 1ng/ml. The study will be useful for studying the immunoassay in the determination of CL residues.

抗体的亚类为IgM，腹水及细胞上清间接ELISA效价分别为1：20 480和1：320，通过竞争阻断实验证明其具有良好的特异性及敏感性，将半抗原经酶标后应用竞争ELISA方法初步建立了CL的检测方法，检测限可达1ng/mL，为进一步制备CL残留检测试剂盒打下了基础。

In the clinic test,a total of 53 field samples were comparatively detected with both GICA and reverse indirect hemagglutination assay,and the positive rate were 95.45% and 90.91%,respectively.

在田间试验中，53份试验样本分别用试纸条和反向间接血凝试验进行检测，2种方法的阳性率分别为95.45%和90.91%。

We examined cellular morphology under phase contrast microscope,cell proliferation using MTT assay,viable cell numbers by hemocytometer counting and cell apoptosis with flow cytometry.

当RA在高浓度(1μM、10μM、100μM)时产生明显抑制作用，细胞形态出现显著变化，48h较24h更明显。

Methods: Human colon cancer cell line DLD1 was treated with Bcl-XL siRNA, The protein level of Bcl-XL in the cells was determined by Western blot; the apoptotic ratio and survival condition were detected by flow cytometry assay and trypan blue-stained cell counting with hemocytometer, respectively. Results: The level of Bcl-XL protein in DLD1 cells was obviously down-regulated by Bcl-XL siRNA.

采用人结肠癌细胞株DLD1，利用人工合成的Bcl-XL靶向小分子干扰RNA转染DLD1细胞，通过Western blot法检测转染细胞Bcl-XL蛋白的表达，流式细胞仪检测处理后细胞的凋亡情况，锥虫蓝染色法计数存活细胞。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。