查询词典 assay

After 4 passages in culture, the MSCs were implanted into 96 foveola with cell density of 0.08,0.1,0.2,0.4,0.6,0.8,1,2,4,6,8,10(x104/ml) respectively. Meanwhile, the MSCs were cultured in Dulbecco's modified Eagle's medium culture medium with different concentration of fetal bovine serum, the 3-4,5dimethylthiazol-2-yl-2,5-diphenyl tetrazelium bromide assay were used to determine the proliferation index of MSCs 72 hours later, the cell cycle were determine by cytometer also.

贴壁筛选法分离、纯化MSCs，取4代以后细胞，以不同密度接种于96孔培养板内，分别以不同浓度血清进行培养，72h后，采用四甲基偶氮唑盐比色法测定MSCs增殖能力并用流式细胞仪测定细胞周期。

Genomic PCR of T0 generation plants revealed that the DNAj gene from foxtail millet was integrated into one wheat plant by repeated assay.

对转化获得的703株T0小麦植株提取DNA进行分子检测，有1株小麦稳定扩增到了预期的1 260 bp的目的基因片段。

Compounds 1 and 2 were new xanthones, and compound 3 was isolated as a natural product for the first time, and compounds 4 and 6 were isolated for the first time from this genus. The antioxidant activities of all compounds were evaluated by ABTS,FRAP and DPPH assays respectively. Compound 9 showed significant activity by the ABTS and FRAP assays. Compound 1 showed significant activity with IC50 value of 0.31 mg·L-1 in DPPH assay.

化合物1，2为新化合物，化合物3为新天然产物，化合物4，6为首次从该属中获得；化合物9在ABTS和FRAP模型中显示出显著的抗氧化活性；化合物1清除DPPH自由基能力的IC50为0.31 mg·L-1。

Methylation assay showed that these sequences are heavily methylated in the Fritillaria ussuriensis.Maxim genome.

甲基化分析则表明这些序列在平贝母的基因组中均被高度甲基化。

The in vitro antifungal assay provided scientific basis for applying GAFP to genetic engineering to prevent and control plant fungous diseases in the near future.

离体抑菌谱的测定为用基因工程的方法防治这些植物真菌病害提供了科学的实验依据。

Methods Keratinocytes monolayers and Malassezia furfur were co-cultured in vitro, and the modulating effects of Malassezia furfur on the expression of TLR-2 was measured by immuocytochemistry methods, the expression and secretion of sICAM-1 and IL-8 in keratinocytes was detected by enzyme linked immunosorbent assay.

马拉色菌和角质形成细胞体外共培养，通过免疫细胞化学方法检测角质形成细胞TLR-2蛋白的表达状况，并运用ELISA方法检测马拉色菌对角质形成细胞sICAM-1和IL-8表达和分泌的调节作用。

Methods On the basis of bilirubin induction and NMDA receptor an-tagonist GAPA administration in guinea pigs. The brain extracellular EAA neurotransmitter was ex-tracted with microdialysis and analysed with HPLC; then, brain tissue ATP was measured by fluores-cence assay and intracellular Ca2 + was measured by fluorescence imaging analysis.

制作胆红素脑病动物模型基础上，在体脑内微透析提取兴奋性氨基酸神经递质，HPLC检测天冬氨酸、谷氨酸、甘氨酸，并给予模型动物NMDA受体拮抗剂GAPA干预，电镜检测脑组织超微结构，荧光检测脑组织ATP与细胞内Ca2+含量。

In an overview,we have confirmed our previous study onα-globin cluster with normal 3C assay.

结果分析表明，绝大部分的Tags是定位到小鼠α-珠蛋白基因簇上的。

The conditions for copper fire assay collection, electrode matching in glow discharge and mass spectrometric analysis were optimized.

着重考察了铜试金条件的选择和辉光放电电极的匹配、质谱的测定条件和测定方法等。

Duffau distillation method for pentosans assay in waste water of wheat glutenin processing was studied.

采用Duffau 蒸馏法对谷朊粉生产废水中的戊聚糖进行测定。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。