查询词典 assay

The results showed: no significant difference was found between the results of single tube quantitative assay method and multiple tube fermentation technique;single tube quantitative assay method showed better accurateness,precision,and reproducibility than multiple tube fermentation technique;single tube quantitative assay method was quicker and more economical.

结果表明：MUG单管定量检测法与大肠埃希氏菌多管发酵法对菌悬液、天然海水、水产品的检测结果没有统计学差异；MUG单管定量检测法检测数据的精确性高于大肠埃希氏菌多管发酵法，而且更为快速、经济。

Significant Spearman rank correlations were found between the results obtained by Luminex assay and by chemiluminescence immunoassay or time-resolved fluorescence immunoassay. Only 1 microliter of serum and 3 hours were needed for Luminex assay. With the advantages of unique capability of multiplexing and high-throughput, good reproducibility, increased detection range and sensitivity, Luminex assay would have a great potential in clinical application.

本研究应用液相芯片技术利用双抗体夹心免疫分析原理同时检测了人血清中的癌胚抗原、甲胎蛋白和乙肝表面抗原，评价了该方法的线性范围、最低检测限、精密度、灵敏度、特异度、准确度等指标，并对检测结果与化学发光免疫分析法（chemiluminescence immunoassay，CLIA）和时间分辨荧光免疫分析法（time-resolved fluorescence immunoassay，TRFIA）进行了相关性分析。

The calibration curve of the SM2-Kit with standard SM2 inhibitor was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 0.625 μg/L to 80.0 μg/L (R2=0.9911), the sensitivity of 0.9 μg/L, the IC50 of 5.82 μg/L and the detection limit of 1 μg/L. The recoveries of SM2 spiked in pig feed were 83.4 %, in pig urine were 89.5 %. The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation was both below 15 %. The SM2-Kit generally had 2.08 % cross-reactivity towards sulfamerazine and little or no cross-reactivity towards other sulfonamides. The dilution solution of SM2 had no effect on results of SM2-Kit. The validity of SM2-Kit in 4 ℃ was above six months.

研究结果表明，SM2-Kit的标准曲线呈典型的S型，相关系数R2=0.9911，符合4参数logit曲线拟合，线性检测范围为0.625 μg/L～80 μg/L，灵敏度为0.9 μg/L，半数抑制浓度(IC50)为5.82 μg/L，检测限为1 μg/L；饲料样、猪尿样的平均添加回收率分别为83.4 %、89.5 %，平均批内和批间变异系数均低于15 %；SM2-Kit与磺胺甲基嘧啶的交叉反应率为2.08 %，与其它磺胺类药几乎没有反应性；基质对SM2-Kit的检测结果影响不大；试剂盒在4 ℃可保存6个月。

Proliferation assay, cellular cycle and apoptosis analysis, invasion and migration assay and adherency assay showed that GEBP11 could inhibit the proliferation of Co-HUVECs and HUVECs, induce the apoptosis of ECs, but not alter the cell cycle of ECs.

MTT细胞增殖实验、细胞周期及凋亡分析、细胞侵袭迁移实验、细胞粘附实验显示，GEBP11短肽对Co-HUVECs及HUVECs的增殖表现出不同程度的抑制作用，对Co-HUVECs抑制作用更强，GEBP11能诱导内皮细胞的凋亡，而对细胞周期无明显影响。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

The immune function was evaluated with T lymphocyte proliferation,NK cell activity assay,IgMplaque forming cell assay,blood serum erythrocytolysin assay and delayedtypehypersensitivity to SRBC.

采用T淋巴细胞增殖和迟发型变态反应来评价细胞免疫功能，采用血清溶血素测定和抗体生成细胞检测来评价体液免疫功能，采用乳酸脱氢酶法测定自然杀伤细胞活性来评价非特异性免疫功能。

Methods The mouse models bearing S180 sarcoma were treated with cadmium chloride with dosages of 0.25,1.00 and 4.00 mg.kg-1,the anti-tumor effect was evaluated by calculating of tumor weight;spleen and thymus indexes,carbon phage assay,lymphocyte transformed assay and hematolysis assay were used to measure the effect of cadmium chloride on immune function.

应用0.25、1.00及4.00 mg.kg-1的氯化镉处理S180肉瘤小鼠模型，检测瘤重确定氯化镉对S180肉瘤的抑瘤作用，采用胸腺指数、脾脏指数、碳粒廓清试验、淋巴细胞转化实验、半数溶血素实验检测小鼠免疫功能的变化。

Considering the merits of in vivo studies in biotransformation i. e. activation or inactivation, protein-binding such as sex hormone binding protein , toxicokinetics, and more evaluative in hazard identification and risk assessment, we selected a battery of short-term and long-term in vivo studies, including uterotrophic assay in weaning mice and ovariectomized adult mice, an in vivo multiple endpoints assay, and a gestational and lactational exposure assay to identify the estrogenic effects of endosulfan on the offspring.

考虑到体内试验在代谢转化、血浆蛋白如性激素结合蛋白的结合、代谢动力学等方面比体外试验优越，在危害鉴定和危险度评定中更有价值，我们选择了多项体内试验，包括对幼年小鼠和卵巢切除成年小鼠的子宫增殖作用、对成年去势小鼠的体内多终点观察以及大鼠孕期和哺乳期接触对雄性子代生殖系统结构和功能的改变，即去雄性化或雌性化作用等，来验证硫丹的体内雌激素样作用。

Methods: NO-3 was restored with cadmium column assay and NO-2 was detected with leavy nitrogen assay. The primitive NO-3 and total restored NO-2(NO-3/NO-2) in plasma of patients with chronic hepatitis and cirrhosis as well as ET-1 with radioimmunology, ALT with Lai's assay were determinated.

取慢性乙型肝炎及活动性肝硬化患者血浆，用镉柱法还原NO-3，重氮法测NO-2，计算血浆中原有及NO-3还原后NO-2总和(NO-2/NO-3)，放免法测ET-1和赖氏法测定ALT。

Result The nested PCR assay was specific and there is no cross-reaction with other parasites, such as Eimeria spp., Cryptosporidium spp., Giardia sp., Toxoplasma sp., Trichuris sp. and cattle ciliate. The assay was able to detect as low as 2.85 × 10^(-2) fg of control positive DNA. The results of detection of clinical samples revealed that the assay was coinciding with microscopic examination.

该套式PCR能特异性地扩增出牛源环孢子虫基因组DNA中相应片段，与艾美耳球虫、隐孢子虫、贾第虫等10种相关原虫基因组DNA无交叉反应；最低能检测到2.85×10^(-2)fg的阳性参照DNA；对临床样品检测结果表明PCR技术检查阳性者显微镜检查都为阳性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。