查询词典 assay

Methods Three-dimensional gels of type I collagen containing rabbit keratocytes were incubated in the presence of different concentrations of exotoxin A (0.1,1.0,10 mg·L-1),cultivated for 24 h at 37℃, the change of keratocytes in morphology was observed under the light microscope,and the amount of lactate dehydrogenase was measured by enzyme linked immunosorbent assay.

含兔角膜基质细胞（1×105·mL-1）三维胶原凝胶表面添加提纯的不同浓度绿脓杆菌外毒素A（0.1、1.0和10 mg·L-1）后，放入无血清MEM培养液中37℃培养24 h，采用光学显微镜观察培养的兔角膜基质细胞形态学变化，采用酶联免疫吸附法检测损伤的兔角膜基质细胞释放的乳酸脱氢酶含量。

The activity of UK was analyzed by fibrinolysis assay.

尿激酶的活性用纤维蛋白溶解试验测定。

The assay was carried out according to the malachite green method, described by Findlay et al.

化验根据孔雀石新方法来进行，在Findlay et al。(1989)中被描述。

Up-regulated proteins under salt stress include an ABC transporter permease, glycerol-3-phosphate permease, pyrimidine nucleotide transporter, formate dehydrogenase, and down-regulated proteins include succinate dehydrogenase iron-sulfur subunit, flavoprotein subunit, cytochrome b-556 subunit, and a hypothetical membrane protein similar to charperone DnaJ. Some of these changes were further verified by enzyme activity assay.

盐胁迫诱导上调表达的蛋白包括ABC型转运蛋白，3-磷酸甘油透性酶，嘧啶核苷转运蛋白和甲酸脱氢酶；下调表达的蛋白包括琥珀酸脱氢酶铁硫亚基、黄素蛋白亚基、细胞色素b556亚基，以及分子伴侣DnaJ的同源蛋白，酶活力测定结果表明胁迫条件下上述蛋白的活性变化与表达量变化相一致。

Accumulated leukocytes were counted in vivo by acridine orange leukocyte fluorography, and retinal vascular permeability was measured by Evans blue assay.

在糖尿病视网膜病变中，视网膜血管内白细胞停滞、聚集是一种较早发生的改变，并可最终导致视网膜屏障破坏[2]。

The effects of As2O3 on HeLa cells and AsPC - 1 cells survival and apoptosis were determined by MTT assay and light microscope, and cellular ROS was also measured by fluorometer.

结果：2μmol／L As2O3处理HeLa细胞48h后细胞的生长受到明显抑制，表现出细胞凋亡的特征，随着As2O3浓度的增加和作用时间的延长效果更加明显。

The chemosensitivity, apoptosis and caspase3 activity of K562 and K562/A02 cells were evaluated by MTT assay, flow cytometer and fluorometer respectively.

以MTT、流式细胞术和荧光分光光度计观察RPL6对化疗药物耐药性、凋亡和caspase3的作用。

The chemosensitivity, apoptosis and caspase??3 activity of K562 and K562/A02 cells were evaluated by MTT assay, flow cytometer and fluorometer respectively.

以MTT、流式细胞术和荧光分光光度计观察RPL6对化疗药物耐药性、凋亡和caspase？？

In vivo tumor metastasis assay, three group cells were inoculated into footpad of 615 mouse subcutaneously. After three weeks, the mice were killed and their lymph nodes were isolated.

结果：1.Gelsolin在小鼠腹水型肝癌细胞系Hca-F细胞和Hca-P细胞胞浆中表达，Western-blot分析显示gelsolin在Hca-F细胞中高表达，在Hca-P细胞中表达较低(P.05)。2。

METHODS: Chemotactic chamber assay was used to investigate the regulatory role of formyl peptide receptor like-1 (FPRL1) in the chemotaxis of PMNs in response to LPS and PD.

用趋化小室方法测定虎杖甙对LPS刺激下多形核白细胞趋化活性及转染了甲酰化肽样受体（FPRL1）基因的293细胞（FPRL1/293）趋化活性的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。