查询词典 assay

The changes of endocardium MAP combined with the blood serum myocardium enzyme assay to diagnose earlier myocardial ischemia and arrythmia is a reliable and fast method.

心律失常时心内膜MAP的特征性形态变化结合血清心肌酶指标是判断早期心肌缺血、心律失常的一种快速、可靠的方法。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

In vitro assay showed that engulfment of apoptotic spermatogenic cells increases ATP production by Sertoli cells.

体外实验证实吞噬凋亡生精细胞能够促进Sertoli细胞产生ATP。

Methods: The expression of p16 protein and nm 23 protein were detected by an immunohistochemistory assay in 130 cases of tissue samples including 30 normal ovary, 50 ovarian benign epithelioma and 50 ovarian epithelial carcinoma.

免疫组化SP法检测30例正常卵巢，50例卵巢上皮性良性肿瘤及50例卵巢癌组织中P16蛋白和nm23蛋白，并分析其阳性表达与肿瘤的关系。

Through Liaoning Province Taian bills farmers first 35 suspected cases of classical swine fever diagnostic, recorded incidence of death from the process and will have a record of 11 symptoms, and so Autopsy examination step analysis, and later in the Shenyang Agricultural University Veterinary College pastoral guidance from the laboratory diagnosis, through production observation, classical swine fever virus onto the world body immune reaction, Enzyme linked immunosorbent assay testing, and made with Erysipelas ,Swine plague, Streptococcosis suis , and Mycoplasmal pneumomia of swine, Swine Toxoplasmosis , and ultimately determine the reasons for the cause of classical swine fever virus incidence.

本人通过对辽宁省台安县某农户的35头猪瘟疑似病例进行诊断，记录了从发病到死亡的过程，并且将所出现的症状一一记录，对死亡的猪挑选了3头进行剖检做近一步分析，对疑似病症进行分析排除。之后提取病料并保存，在沈阳农业大学畜牧兽医学院赵玉军教授的指导下进行了实验室诊断，通过涂片观察、猪瘟病毒兔体交互免疫反应、酶联免疫吸附实验，并做出了与猪丹毒、猪肺疫、猪气喘病、猪链球菌、弓形虫病的鉴别诊断，最终确定为此次引起发病的原因为猪瘟病毒。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

2Examine the chromatograms of the solutions to be examined in the assay. The two principal peaks in the chromatogram obtained with the test solution are similar in retention time to the principal peak in the chromatogram obtained with reference solutions of Estrone Sodium Sulfate and Equilin Sodium Sulfate.

在含量测定项下记录的色谱图中，供试品溶液两个主峰的保留时间应分别与对照品中雌酮硫酸钠和马烯雌酮硫酸钠峰的保留时间一致。

Application of McAbs: the ascites of McAb A_12B_4 were purified with euglobulin precipitation, and tagged with colloidal golds, then the immuno-gold filtration assay was developed to examine avian influenza virus. The results showed that the positive outcome was gained with avian H_9, H_7 and H_5 influenza virus.

单抗的初步应用：A1284株腹水经优球蛋白沉淀法纯化后与胶体金标记，应用于胶体金试纸条检测试验，金标A1284株单抗与H9、H7、H5三种亚型禽流感病毒反应结果呈阳性。

With electromicroscopic-cytochemical assay, comparative observation on the ultracytochemical localization of ATPase in the mesophyll cells of Euonymus radicans was carried out when the seedlings were cold-acclimated or not and then they were treated at -5℃ for 1d.

采用磷酸铅沉淀的电镜细胞化学方法，对经冷驯化（4℃处理）及未经冷驯化（22℃处理）的金边卫矛以及两者在-5℃下处理一天后的叶肉细胞进行了ATP酶的超微细胞化学定位的比较观察。

Seroepidemiologic Studies ;; Measles ;; Rubella ;; Exanthema ;; Antibodies, Viral ;; Fever ;; Disease ;; Immunoglobulin M ;; Enzyme-Linked Immunosorbent Assay

血清流行病学研究；；麻疹；；风疹；；疹；；抗体，病毒；；发热；；疾病；；免疫球蛋白M ；；酶联免疫吸附测定

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。