查询词典 assay

This study shows that the duplex real time PCR assay with the primers for to mecA and nuc genes is a valuable tool for rapid and precise identification of MRSA and methicillin resistant coagulase negative staphylococci.

对mecA、nuc基因进行二重实时PCR能准确、快速地鉴定耐甲氧西林的金葡菌和凝固酶阴性的葡萄球菌。

Mthods: The serum levels of IL-15 were measured by a double-antibody sandwich enzyme-linked immunosorbent assay in 47 severe hepatitis B patients and 20 healthy subjects. Parameters of liver function including alanine aminotransferase and total bilirubin were detected by automatic biochemistry analyzer, meanwhile prothrombin activity were measured by automatic coagulator. In the same time, the hepatitis A, B, C, D and E virus markers were examined in all patients.

方法采用双抗体夹心酶联免疫吸附试验检测47例重型乙型肝炎患者和20例体检健康者血清IL-15的水平，并通过全自动生化仪检测血清丙氨酸转氨酶、总胆红素，全自动血液凝固仪检测凝血酶原活动度，同时采用ELISA方法检测甲、乙、丙、丁、戊型肝炎病原学。

One hundred and eighty 14-days-old chickens were divided randomly into coccidia primary infection group (I1),coccidia secondary infection group (I2),and control group.Immune indexes were examined by using immune staphytococcal protein A rosette test,indirect enzyme-linked immunosorbent assay,cellular culture technique and MTT method.

将180只14日龄雏鸡随机分为毒害艾美球虫初次感染组、二次感染组和对照组，应用免疫SPA菌体花环、间接ELISA及细胞培养技术和四甲基偶氮唑盐测定法对相关免疫学指标进行了检测，以研究毒害艾美球虫二次感染对雏鸡外周血液免疫功能变化的影响。

Methods The compound sulfur facial milk cleanser was made of sulfur, clindamycin hydrochloride, cocoanut oil diethan olamide and triethanolamine etc. Quantitative assay was conducted by titration analysis.

以硫磺、盐酸克林霉素为主药，椰子油单乙醇酰胺、三乙醇胺等药物制成复方硫磺洗面奶；采用化学滴定分析方法测定硫磺的含量。

The mean coefficient of variation for intra and inter batch-assay were less than 10% and 15% respectively.

显著性检验为t检验，批内CV 0 %，批间CV 5 %。

Then the inter assay coefficient of variation was determined by the same instrument and the same brand of kits.

结果：自制的室内质控物高低值的变异范围分别为1.10%、1.01%，稳定性好。

These methods are simple, sensitive and practicable for the assay of coenzyme and examination of related substances.

所建方法简便、灵敏、准确，可用于辅酶Q10的含量测定及有关物质的检查。

A new and quick method for enzyme activity assay was developed in which the reduction of artificial electron acceptor potassium ferricyanide was measured by monitoring the color change of reaction mixture at 420 nm.

为了便于快速简便的测定IDA氧化酶的活力，还建立了一种以铁氰化钾作为人工电子受体，通过检测酶促反应在420nm吸光度的变化而快速定性定量测定酶活力的新方法。

Methods:(1) The levels of IL-2, NK activity in PBMC were measured in 30 patients with primary hepatic carcinoma by MTT colorize assay.

(1)采用MTT比色法检测30例原发性肝癌患者外周血单个核细胞白细胞介素2(IL—2)的诱生水平以及30例原发性肝癌患者外周血单个核细胞自然杀伤细胞活性。

The expression level of metabolitic enzyme (CYP1A1, CYP1B1) and its correlation with cell differentiation /apoptosis were studied as well. The influence of resveratrol in cell cycle was determined with FCM. Results 1 Resveratrol not only suppresses the growth of medulloblastoma cells in a dose and time related fashion but also induces cell differentiation and apoptosis. Resveratrol promotes differention of UW228-1,-2 cells to forward glia, UW228-3 and Med-3 cells to neuron. A treatment of resveratrol in the concentration of 100μM for 48hrs comit most of cells die of apoptosis. 2 Among the four cell lines Fas and Caspase-3 were constantly expessed, whereas FasL was absent irrespective to the drug treatment. 3 ICC, RT-PCR, Western-blot hybridization and EROD enzymatic activity assay demonstrated that expression of CYP1A1 is different after treatment with resveratrol in four cell lines, up-regulated in three UW228 cell lines in a dose dependent manner but down-regulated in Med-3 cells. Suppressive effect of resveratrol on CYP1B1 expression is the same among four medulloblatoma cell lines. 4 Cell cycle distribution of UW228-3 was greatly changed after treatment.

结果：1、白藜芦醇以时间和剂量依赖性方式抑制髓母细胞瘤细胞生长，同时促进细胞的分化和凋亡：白藜芦醇促使UW228-1、-2靶细胞向神经胶质细胞方向成熟分化，UW228-3、Med-3细胞向神经元细胞方向成熟分化，以100μM作用48小时效果显著；2、四个细胞系均有Fas和Caspase-3表达，但无Fas-L基因表达和蛋白活性产生，因此难以形成Fas相关性死亡通路；3、白藜芦醇处理前后CYP1A1基因的表达在各细胞系略有不同：UW228-1，-2，-3三个细胞系白藜芦醇以剂量相关性方式上调CYP1A1的表达，而Med-3细胞系，白藜芦醇却抑制CYP1A1的表达；四个细胞系在白藜芦醇处理后CYP1B1的表达均受到抑制；4、UW228-3细胞系经白藜芦醇作用后细胞周期有较大改变，表现在G0/G1期在整个细胞周期中所占的比例增加，而其它时期则相应减少。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。