查询词典 assay

Chemical methods of qualitative assay and quantitative assay to Chufa functional component According to chemical characteristics reactivity of Chufa functional component, chemical method of qualitative assay and quantitative assay to Chufa functional component were built.

功能因子的定性定量化学分析针对该功能因子的化学特征反应，实验选用硫酸显色法、可见光比色法、吸光度测定法和沉淀法对功能因子进行定性、定量分析。

After mass chemotherapy,ScAg assay in case screen for selective chemotherapy was better than that of ScAb assay and stool examination.ScAg assay was also practice in evaluation of control effect,disease surveillance in children,and to confirm repeat treatment cases.In short,ScAg assay applied for case screen would play an important role in control program.

结论检测ScAg用于全民化疗后选择性化疗的筛查方法优于ScAb及粪便检查，适用于防治效果考核，儿童感染的监测及确定重复治疗对象，经多年应用ScAg筛查血吸虫病对达到传播控制起到重要作用。

Methods compare with sysmex-r3500 analyzer、coulter maxm analyzer、miller ocularto determine 130 ordinary samples.results f=3.87,p.05,the difference is conspicuous.each"r"is 0.7331,0.7231,0.5011,each other are beeline correlation.about sysmex-r3500 analyzer,the inter-assay cv of quality control cv=2.95%,the sample is cv=4.42%;about couter maxm analyzer,the intra-assay cv is 5.70%,intra-assay cv is 17.02%;about miller ocular,the inter-assay cv is 26.15%.conclusion sysmex-r3500 analyzer is good at repeatability,convenience,quickness,so it is adapt to clinical batch samples quickly determine.

分别用sysmex-r3500分析仪，coulter maxm分析仪以及国际血液标准化委员会所推荐的miller窥盘法进行比较，对130份正常人标本进行网织红细胞测定。结果：f=3.87，p.05，差异有显著性。其两两之间r值分别是0.7331，0.7231，0.5011，两两之间直线相关。sysmex-r3500分析仪的质控物批内平均cv=2.95%，标本批内平均cv=4.42%，批间平均cv=3.42%；coulter maxm分析仪批内平均cv=5.70%，批间平均cv=17.02%；miller窥盘法批内平均cv=26.15%。结论：sysmex-r3500分析仪具有重复性好，简便，快捷的特点，尤其适合临床大批标本的快速测定。网织红细胞 miller；窥盘法；仪器法

About SYSMEX-R3500 analyzer,the inter-assay CV of quality control CV=2.95%,the sample is CV=4.42%;About COUTER MAXM analyzer,the intra-assay CV is 5.70%,intra-assay CV is 17.02%;About Miller ocular,the inter-assay CV is 26.15%.

SYSMEX-R3500分析仪的质控物批内平均CV=2.95%，标本批内平均CV=4.42%，批间平均CV=3.42%；COULTER MAXM分析仪批内平均CV=5.70%，批间平均CV=17.02%；Miller窥盘法批内平均CV=26.15%。

An antibody binding to IGF-IR and inhibiting the binding of IGF-I and IGF-II to IGF-IR which is characterized in that said antibody is a is ofIgG1 isotype, b shows a ratio of IC50 values of inhibition of the binding of IGF-I to IGF-IR to the inhibition of binding of IG-II to IGF-IR of 1:3 to 3:1, c inhibits for at least 80% at a concentration of 5 nM IGF-IR phospohrylation in a cellular phosphorylation assay using 3T3 cells providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0.5% heat inactivated fetal calf serum when compared to such an assay without said antibody, and d shows no IGF-IR stimulating activity measured as IGF-IR phophorylation at a concentration of 10 M in a cellular phosphorylation assay using 3T3 providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0,5% heat inactivated fetal calf serum when compared to such an assay without said antibody has improved properties in antitumor therapy.

一种已经提高了抗肿瘤治疗的特性的抗体，所述抗体结合IGF-IR并且抑制IGF-I和IGF-II与IGF-IR结合，其特征在于所述抗体a是IgG1同种型，b显示其对IGF-I与IGF-IR结合的抑制的IC 50 值与其对IGF-II与IGF-IR结合的抑制的IC 50 值的比率为1∶3-3∶1，c当与没有所述抗体的这种测定比较时，其在5nM的浓度上，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3细胞的细胞磷酸化测定中，抑制至少80％的IGF-IR磷酸化，所述3T3细胞提供400，000-600，000分子IGF-IR/细胞，和d当与没有所述抗体的这种测定相比，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3的细胞磷酸化测定中，其在10μM的浓度上没有显示作为IGF-IR磷酸化所测量的IGF-IR刺激活性，所述3T3提供400，000-600，000分子IGF-IR/细胞。

Antioxidant activity of Se-PC was investigated and compared with phycocyanin by using four different free radical scavenging assays, namely, trolox equivalent antioxidant capacity assay, DPPH free radical scavenging assay, superoxide anion scavenging assay and assay for erythrocyte hemolysis mediated by peroxyl free radicals .

通过含硒藻蓝蛋白与藻蓝蛋白对4种不同的自由基清除能力的比较，即总抗氧化能力、清除DPPH自由基、过氧化物阴离子和引发红细胞溶血的过氧化氢自由基能力测定，从而对含硒藻蓝蛋白的抗氧化活性进行评价。

After 5 days, we determined the effects of LF-PMF on EPCs' proliferation ability by MTT assay, cell cycle by flow cytometry, migration ability by cell scrath assay and angiogenic potential by tube formation assay and 3D- culture assay.

曝磁5天后，MTT法检测细胞增殖情况，流式细胞仪检测细胞周期，划痕试验检测细胞迁移能力，管状结构形成试验和三维培养检测细胞成血管能力。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

Methods By using Vero cell as the host cells and with acyclovir as the positive control, anti-HSV-1 activity of the extract was observed and determined with cytopathic effect assay and plaque reduction assay. Initial studies on antiviral mechanism included three aspects: virucidal assay, attachment assay and penetration assay.

以Vero细胞为宿主细胞，阿昔洛韦为阳性对照药物，通过观察细胞病变效应与空斑减数实验测定川楝子提取物抗HSV-1活性，计算其IC50与治疗指数，并从药物对病毒的直接灭活作用、对病毒吸附的影响及对病毒穿膜的影响三个方面初探川楝子提取物抗HSV-1活性的机理。

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。