查询词典 assay

Objective To study sister chromatid exchange fre quency in maternal peripheral lymphocytes and umbilical blood of pregnant women. It is objective to study causes and best assay about PIH.

目的 观察妊娠高血压综合征孕妇外周血及其脐血姐妹染色单体互换频率，探讨PIH的病因以提高PIH的检测水平。

Further more, hoechst stained caveolin-1 expressing GH3 cells showed chromatolysis and the character of cell and cell apoptosis was confirmed by TUNEL assay. To obtain this data, we hypothesize that caveolin may play a role in bromocriptine induced GH3 apoptosis.

在转型48小时后，GH3细胞表现外源性caveolin-1，其细胞呈现萎缩形态且其细胞核呈现计画性死亡的核体分解现象；但表现EGFP的细胞仍维持正常形态。

After washed, the chromogen substrate was added and incubated for 30 minutes in the dark. Rinsed with distilled water, purple spots were observed and counted using a dissection microscope. The number of spots means the level of cytokine producted by sCTL. Analysis of ability of specific cytotoxic lymphocytes to lyse target cells: CytoTox96 Non-Radioactive Cytotoxicity Assay kit was used.

5特异性CTL裂解靶细胞能力测定：采用CytoTox96 Non-RadioactiveCytotoxicity Assay试剂盒，将经HBV core18-27短肽、重组乙肝病毒核心抗原共刺激培养13日的PBMC与HLA-A2〓的靶细胞按一定比例混合，靶细胞被裂解后，释放的乳酸脱氢酶与底物反应显色，酶标仪测定吸光度，吸光度与裂解程度成正比，按照公式计算百分裂解率。

Methods: Parallel-plate-flow-chamber was used to assay the influence of shear stress on endothelial cells morphology and cytoskeleton and apoptosis and NO secretion. endothelial cells morphology was observed under microscopy, cytoskeleton was showed by Coomassie brilliant blue staining, fluorescence stain, and transmission electron microscopy, apoptosis was detected by FCM, NO of circulating culture medium was assayed by NO kit.

细胞形态显微镜下直接观察；细胞骨架通过考马斯亮蓝染色、荧光标记和透射电镜进行观察；细胞凋亡通过流式细胞仪检测进行分析，一氧化氮通过流动循环培养基的收集后应用检测试剂盒进行检测分析。

It was observed that the citrinin assay is specific, no significant cross reactivity was observed in the presence of ochratoxin A.

此外，本压电免疫感测器具良好的专一性，在高浓度的赭麴毒素的存在下并不会产生显著的交叉反应。

At the same time,the effect of injections such as Traditional Chinese Medicine injection of danshen and huangqi on the HPMC in vitro from patients with high coagulaˉtive state was investigated.Methods HPMCs from these patients and normal persons were sored by density gradient Fieoll-Hypaque and cultured48hours in vitro.PCA and PA from these patients and mormal persoms were examined by assay of chromophorous substrate.

分离高凝状态患者及正常人外周血单核细胞，短期培养后用发色底物法检测其促凝血活性和纤溶活性，检测加丹参注射液及黄芪注射液对体外培养HPMC表达PCA和PA的作用。

This study shows that the duplex real time PCR assay with the primers for to mecA and nuc genes is a valuable tool for rapid and precise identification of MRSA and methicillin resistant coagulase negative staphylococci.

对mecA、nuc基因进行二重实时PCR能准确、快速地鉴定耐甲氧西林的金葡菌和凝固酶阴性的葡萄球菌。

Mthods: The serum levels of IL-15 were measured by a double-antibody sandwich enzyme-linked immunosorbent assay in 47 severe hepatitis B patients and 20 healthy subjects. Parameters of liver function including alanine aminotransferase and total bilirubin were detected by automatic biochemistry analyzer, meanwhile prothrombin activity were measured by automatic coagulator. In the same time, the hepatitis A, B, C, D and E virus markers were examined in all patients.

方法采用双抗体夹心酶联免疫吸附试验检测47例重型乙型肝炎患者和20例体检健康者血清IL-15的水平，并通过全自动生化仪检测血清丙氨酸转氨酶、总胆红素，全自动血液凝固仪检测凝血酶原活动度，同时采用ELISA方法检测甲、乙、丙、丁、戊型肝炎病原学。

One hundred and eighty 14-days-old chickens were divided randomly into coccidia primary infection group (I1),coccidia secondary infection group (I2),and control group.Immune indexes were examined by using immune staphytococcal protein A rosette test,indirect enzyme-linked immunosorbent assay,cellular culture technique and MTT method.

将180只14日龄雏鸡随机分为毒害艾美球虫初次感染组、二次感染组和对照组，应用免疫SPA菌体花环、间接ELISA及细胞培养技术和四甲基偶氮唑盐测定法对相关免疫学指标进行了检测，以研究毒害艾美球虫二次感染对雏鸡外周血液免疫功能变化的影响。

Methods The compound sulfur facial milk cleanser was made of sulfur, clindamycin hydrochloride, cocoanut oil diethan olamide and triethanolamine etc. Quantitative assay was conducted by titration analysis.

以硫磺、盐酸克林霉素为主药，椰子油单乙醇酰胺、三乙醇胺等药物制成复方硫磺洗面奶；采用化学滴定分析方法测定硫磺的含量。

Although translator has turned from being a crystal ball by which the original culture can unrestrainedly penetrate to another crystal ball by which the target culture can freely traverse, the translator's personal embodiment, in the process of cognitive act, are still absent in translation studies. Translators are still subjects without body or simply disembodied subjects.

译者虽然由原语文化可以自由穿透的玻璃球变成了译语文化可以自由穿越的玻璃球，但译者认知过程中的个体体验在翻译研究中依然缺席，译者依然仅仅是一个没有躯体体验的主体。

Chillingly, he claimed our technology is 'not nearly as sophisticated' as theirs and "had they been hostile", he warned 'we would be been gone by now'.

令人毛骨悚然的，他声称我们的技术是'并不那么复杂，像他们一样，和"如果他们敌意"，他警告说，'我们将现在已经过去了。