查询词典 assay

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

Growth of cells were observed daily by MTT assay, the status of cell proliferation were observed by Plate colony formation, and the cell cycle of the cells were observed with flow cytometry .

应用MTT比色法绘制细胞生长曲线；克隆形成实验了解细胞增殖情况；流式细胞仪观察细胞周期。

The results of the research are as follows:1 Development of in situ hybridization assay for detection of AIV in MDCK cellA model of viral infection is set up, by infecting MDCK cell with ATV.

以A／V感染MDCK培养细胞，建立了感染模型。

Hyperthermia was applied to the latter,41.5 degrees Centi grade, 1 h, after having received same irradiation. The cel surviving fractions were determined by clonogenic assay and the parameters of the multi-target/single-hit model, Do, D9, as well as those of the linear-quadratic model, ratio of alpha and beta, were elicited.

集落形成率实验检测两组细胞在不同情况下的细胞存活分数，分别根据多靶单击模型和线形二次模型拟合绘制细胞存活曲线，求出 D0、Dq及α/β比等细胞存活曲线参数，并获得照射2 Gy时的存活分数SF2。

Antigangliosides GM1 antibody and cephalin antibody in serum (n=28) and cerebrospinal fluid (n=18) were examind in MS patients with ELISA assay.

采用ELISA方法测定多发性硬化患者活动期血清(28例)和脑脊液(18例)的GM1抗体、脑磷脂抗体和髓鞘碱性蛋白。

Objective In order to understand the role of the immune function in the pathogenetic mechanism of multiple sclerosis. Method Antigangliosides GM1 antibody and cephalin antibody in serum (n=28) and cerebrospinal fluid (n=18) were examind in MS patients with ELISA assay.

目的 探讨多发性硬化的免疫发病机制方法采用ELISA方法测定多发性硬化患者活动期血清(28例)和脑脊液(18例)的GM1抗体、脑磷脂抗体和髓鞘碱性蛋白。

The Smart CMV assay was launched by Cepheid for clinical diagnostic use on its SmartCycler system.

Smart CMV检测是由Cepheid公司(Sunnyvale， CA， USA)推出用于该公司SmartCycler系统临床诊断用途。

Dot blotting assay was employed in this study to ob- serve dynamic changes of c-fos mRNA expression during reperfusion after cere- bral ischemia in rats.

本实验用改良的异硫氰酸胍一步法从脑组织中提取总RNA，与α—〓标记的c—fos cDNA（2.1 kb）探针进行斑点杂交，观察大鼠脑缺血过程中c—fos mRNA表达的变化及小檗碱对其的影响。

Megaterium/B. cereus group and 8 products of MF coincided with the biochemical assay.

同时对10株待测菌株和8个微生物肥料产品进行检测，其鉴定结果与常规鉴定结果一致。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。