查询词典 assay

It is apparent that the VirB8-PCR assay appears to be specific and sensitive and can be used for the detection of the virulence factor of Brucella as well as the surveillance of brucellosis.

VirB8-PCR方法特异、敏感，能用于布鲁氏菌病的监测和致病力因子的检测。

METHODS:The expression of Ki67 in three breast caner cell lines was detected by half-quantitative RT-PCR and Western blot assay.

采用半定量RT-PCR和Western blot分别检测3株乳腺癌细胞中Ki67mRNA和蛋白表达水平。

Methods MTT assay was used to determine the proliferation inhibition by cantharidin to A549 cell. FACS can were applied to detect the change of cell cycle.

采用MTT法检测斑蝥素对A549细胞增殖的抑制作用；流式细胞仪分析A549细胞周期及斑蝥素对细胞周期的影响。

An assay for carbonic anhydrase enzyme, which is thought to play an important role in the process of bio-calcification revealed novel activity.

在对生物钙化过程中起重要作用的碳酸酐酶的分析中显示了此酶的新颖的活性。

The expressions of HO-1 and HO-2 mRNA were detected using reverse transcription polymerase chain and the relative amount of CO released into the media was quantitated as carboxyhemoglobin by enzyme linked immunosorbent assay.

通过逆转录聚合酶链式反应和免疫细胞化学法检测HO-1、HO-2 mRNA和蛋白的表达，酶联免疫法检测培养上清液中碳氧血红蛋白含量，四唑氮比色法检测细胞增殖。

Objective To research the specificity of antigen from group A streptococcal cell membrane in procoagulant activity assay for the diagnosis of rheumatic carditis.

目的 研究A组乙型溶血性链球菌菌膜抗原在外周血淋巴细胞促凝血活性试验中诊断风湿心肌梗死性心脏炎的特异性。

The effect of carotenoid to proliferation cycle of breast cancer cell was detected by flow cytometer assay.

采用流式细胞仪检测类胡萝卜素对人乳腺癌细胞增殖周期的影响。

Hematology levels of CD40L, CRP, MMP-2 and MMP-9 of all 330 patients were measured by enzyme-linked immunosorbent assay, respectively, before and 1 and 6 months as well as 1 year after CASI.

对所有患者分别于术前，术后1个月，6个月，1年，采用ELISA法测定CD40L、C反应蛋白、MMP-2、MMP-9。

Methods Enzyme-linked immunosorbent assay was used to detect the level of serum CRP and CA125 in 38 patiens wih active phase UC, 35 patients with catabasis UC and 39 healthy individuals.

采用ELISA方法检测38例活动期UC患者、31例缓解期UC患者及69例健康对照者血清CRP及CA125水平。

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。