查询词典 assay

Objective To research the specificity of antigen from group A streptococcal cell membrane in procoagulant activity assay for the diagnosis of rheumatic carditis.

目的 研究A组乙型溶血性链球菌菌膜抗原在外周血淋巴细胞促凝血活性试验中诊断风湿心肌梗死性心脏炎的特异性。

The effect of carotenoid to proliferation cycle of breast cancer cell was detected by flow cytometer assay.

采用流式细胞仪检测类胡萝卜素对人乳腺癌细胞增殖周期的影响。

Hematology levels of CD40L, CRP, MMP-2 and MMP-9 of all 330 patients were measured by enzyme-linked immunosorbent assay, respectively, before and 1 and 6 months as well as 1 year after CASI.

对所有患者分别于术前，术后1个月，6个月，1年，采用ELISA法测定CD40L、C反应蛋白、MMP-2、MMP-9。

Methods Enzyme-linked immunosorbent assay was used to detect the level of serum CRP and CA125 in 38 patiens wih active phase UC, 35 patients with catabasis UC and 39 healthy individuals.

采用ELISA方法检测38例活动期UC患者、31例缓解期UC患者及69例健康对照者血清CRP及CA125水平。

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

Growth of cells were observed daily by MTT assay, the status of cell proliferation were observed by Plate colony formation, and the cell cycle of the cells were observed with flow cytometry .

应用MTT比色法绘制细胞生长曲线；克隆形成实验了解细胞增殖情况；流式细胞仪观察细胞周期。

The results of the research are as follows:1 Development of in situ hybridization assay for detection of AIV in MDCK cellA model of viral infection is set up, by infecting MDCK cell with ATV.

以A／V感染MDCK培养细胞，建立了感染模型。

Hyperthermia was applied to the latter,41.5 degrees Centi grade, 1 h, after having received same irradiation. The cel surviving fractions were determined by clonogenic assay and the parameters of the multi-target/single-hit model, Do, D9, as well as those of the linear-quadratic model, ratio of alpha and beta, were elicited.

集落形成率实验检测两组细胞在不同情况下的细胞存活分数，分别根据多靶单击模型和线形二次模型拟合绘制细胞存活曲线，求出 D0、Dq及α/β比等细胞存活曲线参数，并获得照射2 Gy时的存活分数SF2。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。