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MTT assay and -TdR incorporation were used to test the proliferation of HepG2 cell caused by augmenter of liver regeneration.Inorganic phosphorus spectrophotometer was used to test the activities of the Na~+,K~+ATPase.

采用四甲基噻唑盐法检验肝再生增强因子对H epG 2细胞增殖作用；[3H]-T dR掺入测定细胞DNA合成；采用无机磷比色法测定细胞N a+，K+-ATPase的酶活力。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

OBJECTIVE To assay retrospectively the isolation rate and susceptibility of Staphylococcus aureus in our hospital during five years.

目的 对近5年分离的金黄色葡萄球菌的药敏结果进行回顾性分析，为抗菌药物的正确选用提供依据。

Objective To detect the level of anti-SSA-60 000 autoantibody in patients with autoimmune diseases as well as its assay methods.

目的 建立便捷的检测抗干燥综合征A抗原(分子量为60 000的多肽成分)的自身抗体的方法，以利于疾病的早期诊断和病程监控。

Methods The TTF-1 DNA binding activity was detected in lung cancer tissue by Electrophoretic mobility shift assay, autoradiography and photo densitometry.

本研究观察在肺癌组织中TTF-1的DNA结合活性，探讨其与肺癌病理类型、分化程度的关系。

The levels of DAG were investigated with radioenzymatic assay, thin-layer chromatography and autoradiography.

上述信号通路的变化效应，可被anti-CD40MAb阻断，这就进一步证实，rCD40L引起的DAG-PKC途径的激活是通过CD40而起作用的。

Four positive clones were obtained by the selection of autotrophic phenotype and β-galactosidase assay.

利用营养缺陷型和β—半乳糖苷酶活性筛选到4个阳性克隆，测序结果表明它们具有相同的序列。

To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum（1.562 5-200 ng/ml）. Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment （350 bp）had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性，采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度，取高纯度的DNA样品用灭菌水稀释，分别取不同量的DNA进行PCR扩增，确定PCR方法的敏感性；利用该方法对32份奶牛流产的胎牛脑组织样品进行检测，同时，对其中23份流产的母牛血样进行ELISA血清学检测，以评价犬新孢子虫PCR方法的检测效果。

Methods The Ames test, micronucleus test and comet assay were used to detect the genotoxicity of bactericide flutriafol.

应用Ames试验、小鼠骨髓嗜多染红细胞微核试验、慧星试验研究粉唑醇的遗传毒性。

Methods The RBC of bacteriuria or abacteriuria was determined by UF-100 and microscopy, MIDITRON and monoclonal assay.

目的 观察菌尿对尿液分析仪(全自动尿沉渣分析仪UF-100和MIDITRON尿液分析仪)测定红细胞的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。