查询词典 assay

To study the mechanism, a stable and credible assay system to measure gene conversion is of great importance.

因此，揭示寡核苷酸介导的基因定点修复机制是该策略研究的重要内容。

At first, we studied the assay of cellulase activities because there is not a standard.

首先，针对目前纤维素酶活力的测定没有统一的标准，测定结果无法比较的现状，论文的第一部分对纤维素酶活力的测定进行了研究。

Observing the function of astrocyte in damage part with the method of γ-GT activity assay.

GT测定，以观察脊髓损伤处的星形胶质细胞功能情况。4。

The OsCMO contains 10 exons and 9 introns and encodes a protein with 410 amino acids,which shows 62﹪, 51﹪, 51﹪, 51﹪ and 50﹪ identities in amino acids to CMOs from Arabidopsis thaliana,Atriplex hortensis, Amaranthus tricolor, Suaeda liaotungensis and Beta vulgaris respectively. Semi-quantitative RT-PCR assay revealed OsCMO was significantly up-regulated in the rice seedlings under salt, cold and drought stresses. It suggests that OsCMO might play an important role in rice responses to abiotic stresses.

该基因含有10个外显子和9个内含子，其开放阅读框编码一条长为410个氨基酸残基的多肽，与拟南芥、山菠菜、苋、碱蓬和甜菜等植物的胆碱单加氧酶的氨基酸序列的一致性50﹪～62﹪。mRNA分析表明OsCMO基因在水稻幼苗组织中的表达受高盐、低温和干旱等胁迫的上调诱导表达，表明该基因可能在抵御非生物胁迫中发挥重要作用。

The objective of this study was to evaluate hearing threshold shifts and DNA damage using Pure-tone audiometry and comet assay in paper recycling workers who had been chronically exposed to noise in the workplace.

过去的研究已显示废纸回收造纸厂的噪音作业环境会引起不同程度的听力损失，但并没有相关的研究显示是否会引起DNA损伤。

MTT assay and -TdR incorporation were used to test the proliferation of HepG2 cell caused by augmenter of liver regeneration.Inorganic phosphorus spectrophotometer was used to test the activities of the Na~+,K~+ATPase.

采用四甲基噻唑盐法检验肝再生增强因子对H epG 2细胞增殖作用；[3H]-T dR掺入测定细胞DNA合成；采用无机磷比色法测定细胞N a+，K+-ATPase的酶活力。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

OBJECTIVE To assay retrospectively the isolation rate and susceptibility of Staphylococcus aureus in our hospital during five years.

目的 对近5年分离的金黄色葡萄球菌的药敏结果进行回顾性分析，为抗菌药物的正确选用提供依据。

Objective To detect the level of anti-SSA-60 000 autoantibody in patients with autoimmune diseases as well as its assay methods.

目的 建立便捷的检测抗干燥综合征A抗原(分子量为60 000的多肽成分)的自身抗体的方法，以利于疾病的早期诊断和病程监控。

Methods The TTF-1 DNA binding activity was detected in lung cancer tissue by Electrophoretic mobility shift assay, autoradiography and photo densitometry.

本研究观察在肺癌组织中TTF-1的DNA结合活性，探讨其与肺癌病理类型、分化程度的关系。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。