查询词典 assay

ChinaObjective:To study the antitoxic role of vesicular monoamine transporter 2 (VMAT2) in transpgenic Chinese Hamster ovary cell.Methods:With the technology of transgene from PC12 to CHO,MTT reduction assay was used to detect MPP^+ toxic effect on wild type CHO and transgenic CHO.

利用转PC12细胞基因到CHO细胞中形成的转基因CHO细胞（cDNACHO，采用MTT比色法检测1-甲基-4-苯基吡啶离子（MPP^+对CHO细胞野生株（wtCHO和cDNACHO细胞的毒性作用，并观察利血平-VMAT2的特异性阻滞剂对MPP^+毒性作用的影响。

Methods:All of 40 male Sprague-Dawley rats were divided into 3 groups:control group（group A, n = 10）, diabetic group for 4 weeks（group B, n = 14）, and diabetic group for 12 weeks（group C, n = 16）. The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NF-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组：A组为正常对照组（10只），B组为糖尿病4周组（14只），C组为糖尿病12周组（16只），采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后，A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能，并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达，同时采用电泳迁移率变动分析法进行NF-κB 活性检测。

Methods All of 40 male Sprague-Dawley rats were divided into 3 groups: control group (group A, n=10), diabetic group for 4 weeks (group B, n=14), and diabetic group for 12 weeks (group C, n=16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NE-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组：A组为正常对照组（10只），B组为糖尿病4周组（14只），C组为糖尿病12周组（16只），采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后，A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能，并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达，同时采用电泳迁移率变动分析法进行NF-κB活性检测。

Objectives To investigate the functions of dopamine D: and D2 receptor in form deprivation myopia and the influcnce of apomorphine on inhibition of FDM in chick with rafiligand bgading assay.

目的 采用放射受体分析方法测定鸡视网膜多巴胺受体的理化特性，研究多巴胺系统与剥夺性近视和药物治疗近视相关的机理。

The total genomic DNA of diseased apple leaves was rapidly obtained by modified CTAB method. Through PCR amplification assay, the pathogen of apple scab can be specifically detected from diseased apple leaf tissues and no symptom appearance of apple leaf tissues with pathogen incubation.

采用改进的CTAB法，快速提取发病叶片组织的DNA，并结合PCR检测技术，可从苹果黑星病显症叶片以及未显症而处于潜育期的叶片组织中特异性地检测到苹果黑星病菌。

Then tested brain-gut peptides content in blood and gastric antrum with radioimmunopercipitation assay and 5- HT APUD cell figure with immunohistochemical method and brain-gut peptides receptor mRNA express with orthotopic hybridization.

旋复代赌汤各剂量组和吗丁琳组与两个模型组比较，血液中ss含量明显降低，有显著性差异(P0.05)；旋复代储汤中、高剂量组及吗丁琳组与模型3天组比较，组织中55含量降低，有显著性差异(P0.05)。

Objective: To establish a reverse-phase high performance liquid chromatographic assay for the determination of vacomycin levels in rabbits aqueous humor.

目的：应用反相高效液相色谱技术，建立定量检测兔房水万古霉素的方法。

To assay the activity of matrix metalloproteinases in the aqueous humor by the same way in four groups of albino rabbit eyes treated with Lumigan for 3, 7, 14, 21 days respectively and the control group treated with normal saline.

用同样的方法检测滴用Bimatoprost 3、7、14、21天后兔眼房水中MMPs的变化，并应用UVI凝胶电泳定量分析系统进行各个条带的定量分析。

Then the expression of ICAM-1 incubated with or without areca nut extract in the presence of 10％ fetal calf serum for 48 h at 37 ℃ in 5％ CO2 and air were measured by using enzyme-linked immunosorbent assay.

分别从正常及 OSF患者的口腔黏膜中培养出 FB，向培养基中加入不同浓度的 ANE培养 48 h后，用细胞酶联免疫方法检测 FB表达的 ICAM-1水平。

MTT assay appears to be useful in exploring the possible anticancer effect of arsenic trioxide on tongue cancer.

。MTT法对新药的临床应用也有较好的指导作用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。