查询词典 assay

This study was designed to investigate the mechanisms of histone deacetylase inhibitors(sulfonamide anilide compound referred as the "Compound") with antitumor activity against human bladder cancer cells. MATERIALS AND METHODS: Treated with different concentrations of the Compound and HCPT for different periods of time, human bladder cancer cell line BIU-87 growthwas measured by MTT assay.

材料与方法：以不同浓度的Compound和羟基喜树碱作用于体外培养的膀胱癌BIU-87细胞株；采用MTT法检测细胞生长抑制率；以流式细胞术测定不同浓度的Compound和HCPT作用前后膀胱癌细胞的调亡情况及细胞周期的变化；应用RT-PCR分析Compound和HCPT作用前后膀胱癌细胞中P21waf/cif1 mRNA表达的变化。

This study was designed to investigate the mechanisms of histone deacetylase inhibitors (sulfonamide anilide compound referred as the Compound') with antitumor activity against human bladder cancer cells. MATERIALS AND METHODS: Treated with different concentrations of the Compound and HCPT for different periods of time, human bladder cancer cell line BIU-87 growthwas measured by MTT assay.

材料与方法：以不同浓度的Compound和羟基喜树碱作用于体外培养的膀胱癌BIU-87细胞株；采用MTT法检测细胞生长抑制率；以流式细胞术测定不同浓度的Compound和HCPT作用前后膀胱癌细胞的调亡情况及细胞周期的变化；应用RT-PCR分析Compound和HCPT作用前后膀胱癌细胞中P21(上标 waf/cif1)mRNA表达的变化。

The polysaccharide and glucan contents were analyzed by phenol-sulfuric acid method and aniline blue dye fluorescence assay, respectively.

首先将液态灵芝发酵液和商业灵芝粉以酚硫酸法测多醣产量，再以萤光分析方法进行葡聚醣定量。

The in vitro assay results showed that SGF4A had a good anticoagulant activity.

体外实验表明，SGF4A具有较好的抗凝活性。

The effects, of protection and treatment with, combined Ch, NaF and a trophie were significantly better than that with atropine alone. In isolated phrenic nerve-diaphragm preparation of rat, isolated jejuneal preparation of, guinea pig, and the metabolic experiment of rat liver, the results of the, assay on the rat blood ChE activity indicated that antidotal effect of NaF may be due to the antidese...

大鼠离体膈神经膈肌、豚鼠离体空肠和大鼠肝脏代谢实验及对大鼠全血胆碱酯酶活力测定的结果表明，NaF的抗毒作用可能是由于其对神经肌肉接头和/或神经节中的N受体的抗脱敏作用；而Ch则可能通过其对ChE的保护和/或加速被抑制ChE的复能，阻碍Ach到达M受体部位而发挥作用。

But the antigenicity of HBc in MIR fusion protein was too low to assay.

但检测不到HBcAg的抗原性。

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P.01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48 and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%. The overall sensitivity was significantly higher (P.05) than that of modified indirect MAIPA and that of using single antibody.

结果 特发性血小板减少性紫痕组4种羊抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P.01)；若将ITP组患者4种羊抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性，则流式微球技术检测血小板特异性自身抗体的敏感性为73.2%，特异性为94.3%；4种羊抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P.05)，且大于各单个抗体检测敏感性。

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P .01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48, and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%.

结果 特发性血小板减少性紫癜组4种单抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异（P＜0.01）；若将ITP组患者4种单抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性，则流式微球技术检测血小板特异性自身抗体的敏感性为73.17%，特异性为94.3%；4种单抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验（P＜0.05，且大于各单个抗体检测敏感性。

By series of in vitro tests, including DPPH radicals and hydroxyl radicals scavenging activity assays, reducing power test, anti-superoxide radicals assay, anti-human low density lipoprotein oxidation test, protections against hydroxyl radical- mediated DNA damages and peroxynitrite medialed dihydrohodamine oxidation, it was showed that TIs exhibited antioxidant and antiradical activities compared with positive controls.

藉由一系列抗氧化DHR123活性试验，如清除DPPH自由基、清除hydroxyl自由基、还原力的测定、清除超氧自由基的测定、抗低密度脂蛋白氧化、抑制peroxynitrite氧化和保护DNA氧化等研究，都显示TIs具有抗氧化活性。

Heparin and antithrombin activity were evaluated by an anti-Xa assay and measurement of antithrombin III activity.

肝素和抗凝血酶活性通过抗-Xa 检测和抗凝血酶Ⅲ活性测定来确定。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。