查询词典 assay

In this study,we used agglutination inhibition assay and affinity proteomic technology to further investigate the agglutinative target of hemocyanin.

本研究采用凝集抑制实验和亲和蛋白质组学等方法探索凡纳滨对虾血蓝蛋白与病原菌的凝集作用靶标。

Methods The dot immunogold filtration assay kit was developed and potato agglutinin was applied to blot blood quickly.

摘要目的？摇建立一种检测全血标本的棘球蚴病现场快速诊断方法。

ALB and blood lipid were determined by immune turbidimetric assay and the ALB level was compared among the groups.

动脉血压中的收缩压、舒张压及平均动脉压是评定高血压严重性的较好指标。

Meanwhile we observed the change of symptoms, biopsy, T cell inferior group of serum (CD3、 CD4、CD8)、immunoglobulin (IgG、IgA、IgM) and the alexin (C3、C4), cAMP、 cGMP. CD3、CD4、CD8 are detected by immunomomoclonal antibodies APPAP; IgG、IgA、IgM are tested by immunoturbidimetry assay; the content of cAMP and cGMP in blood plasm are tested by radioimmunoassay.

所有观察病例于治疗前均作血、尿、大便常规化验；心、肝、肾功能检查；阴道分泌物检查包括念珠菌、滴虫、阴道清洁度；尿糖、血糖检查；分别观察中药组和西药组治疗前后相关症状及体征变化情况，活体组织病理检查的变化情况；血清T淋巴细胞亚群（CD3、CD4、CD8）和免疫球蛋白(IgG、IgA、IgM)以及补体（C3、C4）各项指标的变化情况；血中cAMP、cGMP含量变化情况，T淋巴细胞亚群水平测定采用单克隆抗体APAAP桥联酶标技术，血清免疫球蛋白IgG、IgA、IgM及补体C3、C4的测定采用免疫透射比浊法。

Following confirmation by RFLP and partial sequence analysis, the aliquot mutant viruses (3μg) were used to transfect CEM×174 cell line, and the concentration of SIVmac main core protein(P27) in culture supernatants was closely monitored by enzyme-linked immunosorbent assay in order to identify the replicating ability of the mutants.

一般认为，外膜糖蛋白在病毒的感染和免疫中起重要作用，而SIVmac239和SIVmac142克隆之间envGP区核苷酸的差别，是否提示SIVmac239克隆的致病性或毒力与envGP全区、部分或个别核苷酸有关，仍需要进一步研究〔2，3〕。

In order to understand the mechanism of MTX further and to investigate the genotoxic target organs, we studied the DNA damage and the correlation with dose of MTX by using the alkaline single cell gel electrophoresis assay.

单细胞凝胶电泳技术是近十几年来开展的一项检测有核细胞DNA损伤的新技术，在遗传毒理学和生物学监测等领域得到广泛应用。

The proliferation of osteoblasts,assay of ALP activity,osteocalcin were detected and acquired.

采用RT-PCR和Western Blot的方法检测PCNA基因蛋白的表达。

Objective To explore the roles ofα-amanitin in the replication ofhepatitis B virus in HepG2 2.2.15 cell line Methods MTT assay were used to observe the cytotoxicity ofα-amanitin to the HepG 2 2.2.15 cell line.

中文摘要：目的：基于α-鹅膏毒肽的作用机制和乙肝病毒复制转录特点，初步探讨α-鹅膏毒肽对HepG2.2.15细胞的毒性及其对乙型肝炎病毒复制的影响。

Here we show that VacA permeabilizes the apical membrane of gastric parietal cells and induces hypochlorhydria.The functional consequences of VacA infection on parietal cell physiology were studied using freshly isolated rabbit gastric glands and cultured parietal cells.Secretory activity of parietal cells was judged by aminopyrine uptake assay and confocal microscopic examination.

为此，我们建立了原代培养的胃腺、胃壁细胞等研究模型和良好的评估壁细胞分泌能力的技术平台，包括氨基比林吸收实验、激光共聚焦扫描技术等，对VacA感染所导致的壁细胞生理特征的改变进行了深入细致的研究。

CA II and AQP5 expression levels were determined by immunofluorescence and immunoblotting, while CA activity and Na, K-ATPase activity by a colorimetric assay. Anhidrotic mice treated by topiramate did not differ from controls in average secretory coil diameter, CA II expression, CA activity and Na, K-ATPase activity.

应用免疫荧光和western blot技术发现：小鼠在托吡酯处理后泌汗减少，碳酸酐酶同功酶II型(carbonic anhydraseII，CAII)蛋白表达未出现显著变化，但汗腺组织胞膜的水通道蛋白-5(aquaporin-5，AQP5)表达量下调。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了