查询词典 assay

An automated assay was used to analyse serum AAP activities of 229 patients with hepatobiliary diseases and 70 normal individuals.

用自动分析仪速率法测定 2 2 9名肝胆疾病患者和 70名健康人血清AAP活力。

Purpose and Methods : In this study , the micronucleus test and comet assay of V ice f aber were used to screen pollution situation of the surface water of Yangzhong city.

目的与方法：本文应用蚕豆根尖微核技术和彗星试验对扬中市不同水体的遗传毒物污染状况进行了初步调查。

Objective To study the diagnostic values of staphylococcal protein A-enzyme linked immunoadsorbent assay and mite antigen piece-indirect fluorescent antibody test on pulmonary acariasis.

目的 探讨葡萄球菌A蛋白-酶联免疫吸附试验和螨成虫抗原片间接荧光抗体试验对肺螨病的诊断价值。

The topic is on the basis of a hydrochloric acid mist emissions from factories and design of an assay using the general industrial emissions of hydrochloric acid Department Jimmy installation that acid absorber.

本课题就是根据某工厂排放盐酸雾的情况而设计的一种利用吸附法对一般工业盐酸废气的处理装置，即酸雾吸附器。

By using the chlortetracycline assay, we found the effect of GABA on rat spermatozoa acrosome reaction is biphasic, i.

本研究则进一步分析这两种受体在大鼠精子顶体反应中的作用机制和相互关系。

General condition, wound area, and histological characters were observed, as well as the proliferation of T and B lymphocyte by MTT assay, phagocytosis of Mφ in abdominal cavity by neural red assay were investigated respectively.

结果如下： 1 整体试验 1.1 免疫抑制复合皮肤伤口模型的复制-阳虚型愈合迟缓性皮肤伤口以氢化考的松处理小鼠造成免疫抑制状态，用特制打孔器在鼠背打一圆孔，采取全厚皮切除术，复制ISW模型。

The recombinant fowl poxvirus rFPV-E0-E2 was serially passaged for 20 passages in specific-pathogen-free chicken embryo fibroblast culture. The passage 5, 10, 15 and 20 were chosen to identify by blue plaque assay, PCR amplification, gene sequence analysis and indirect immunofluoresence assay.

将重组鸡痘病毒rFPV-E0-E2在鸡胚成纤维细胞上连续传20代，取第5、10、15和20代重组病毒进行以下检测：用蓝斑试验鉴定各代重组病毒纯度；用PCR方法扩增猪瘟病毒E0、E2基因，进行基因序列分析；用间接免疫荧光实验检测各代重组病毒中猪瘟病毒E0、E2基因的表达。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

As an example, the relative standard deviations of the intra-assay and inter-assay of polymyxin E1 on the plate number and peak area were less than 5%.

以多粘菌素E1为例，柱效和峰面积的日间及日内测定的相对标准偏差均小于5%。E1和E2在硫酸多粘菌素E药物中的含量分别为67%和32%。

This novel real-time enterovirus assay is a sensitive and suitable assay for routine clinical testing.

这种实时定量PCR的方法是足够敏感和稳定满足常规的临床样本分析的。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了