查询词典 assay

In BP exposure experiment, except that the neutral red assay showed that BP significantly inhibited the haemocyte viability, the other methods did not detect the inhibition effect, besides, the results of the neutral red assay showed that the inhibition degree had a positive correlation with the concentration and exposure time of BP.

采用中性红比色法检测BP对栉孔扇贝血细胞活性具有显著抑制作用，且抑制程度与BP作用浓度、时间呈正相关，其余方法均未检测出BP对血细胞活性的影响。

DNA synthesis rate was assessed by performing tritiated thymidine incorperation assay. The expression of SM-α-actin and proliferation of DSMC were analyzed by immunofluorescent assay and flow cytometory.

结果显示，逼尿肌细胞的形变量和粘弹性并不呈线性关系，一定限度内的张力负荷（拉伸20％）粘弹性系数下降，而继续加大张力（拉伸30％）时变化并不明显。

Methods: rabbit bone marrow mesenchymal stem cells were purified, and then cultured in inducing medium containing transforming growth factor-β1; mtt assay was employed to evaluate the proliferative activity of induced cells; immunohistochemical assay was used to detect the expression of type ⅱ collagen; induced cells were transplanted into the knee of rabbit, and x-ray photography was employed to observe the repair and formation of cartilage.

纯化兔骨髓间充质干细胞；用含转化生长因子β1的诱导液培养骨髓间充质干细胞；以mtt测定诱导细胞的增殖活性；免疫组化检测ⅱ型胶原蛋白的表达；诱导后的细胞移植于白兔膝关节内，x线摄片观察软骨修复形成情况。

The proliferation of Schwann cells of sciatic nerves was determined in different time by MTT assay and Thymidine incorporation assay.

结果 人参皂甙 Rb1 在 10μg/ ml的浓度对雪旺细胞增殖有明显促进作用，高浓度的人参皂甙 Rb1 1mg/ ml则显示抑制作用。

General condition, wound area, and histological characters were observed, as well as the proliferation of T and B lymphocyte by MTT assay, phagocytosis of Mφ in abdominal cavity by neural red assay were investigated respectively.

结果如下： 1 整体试验 1.1 免疫抑制复合皮肤伤口模型的复制-阳虚型愈合迟缓性皮肤伤口以氢化考的松处理小鼠造成免疫抑制状态，用特制打孔器在鼠背打一圆孔，采取全厚皮切除术，复制ISW模型。

The recombinant fowl poxvirus rFPV-E0-E2 was serially passaged for 20 passages in specific-pathogen-free chicken embryo fibroblast culture. The passage 5, 10, 15 and 20 were chosen to identify by blue plaque assay, PCR amplification, gene sequence analysis and indirect immunofluoresence assay.

将重组鸡痘病毒rFPV-E0-E2在鸡胚成纤维细胞上连续传20代，取第5、10、15和20代重组病毒进行以下检测：用蓝斑试验鉴定各代重组病毒纯度；用PCR方法扩增猪瘟病毒E0、E2基因，进行基因序列分析；用间接免疫荧光实验检测各代重组病毒中猪瘟病毒E0、E2基因的表达。

After analyzing the Sp1 DNA sequence within 5'-UTR and promoter region, we found one typical IRES-conserved sequence localizing within 5'-UTR of Sp1 genomic sequence. The reporter assay and polysome distribution assay revealed that Sp1 could be accumulated strongly and rapidly under hypoxia/ischemia neuron cells.

在Polysome distribution assay的结果显示，Sp1在缺糖缺氧之后转译效率有提高的情形，接著分析Sp1 5'-UTR，发现可能存在一个典型的IRES结构，利用bicistronic assay也证实了Sp1的堆积是透过IRES所导致。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

As an example, the relative standard deviations of the intra-assay and inter-assay of polymyxin E1 on the plate number and peak area were less than 5%.

以多粘菌素E1为例，柱效和峰面积的日间及日内测定的相对标准偏差均小于5%。E1和E2在硫酸多粘菌素E药物中的含量分别为67%和32%。

This novel real-time enterovirus assay is a sensitive and suitable assay for routine clinical testing.

这种实时定量PCR的方法是足够敏感和稳定满足常规的临床样本分析的。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。