查询词典 assay

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

The level of ifn γ secreted from splenocytes of immunized mice after specific antigen stimulation was detected by a cytopathic effect reduction assay and the splenocytic proliferation of immunized mice in response to antigen restimulation was detected by mtt assay.

分离免疫小鼠的脾淋巴细胞，体外用抗原再刺激，mtt方法检测ifn γ产生水平和mtt法检测淋巴细胞增殖，并对脾脏细菌负荷计数。

Methods: Eleven B-CLL patients were studied. Leukemic lymphocytes with (n=8) or without (n=3) P2z receptors were exposed in vitro to ATP, benzoylbenzoic-ATP, 2-methylthio-ATP(2MeSATP), adenosine-5′-［γ-thio］ triphosphate, and other nucleosides for 8h. Apoptosis was detected by electron microscopy, agarose gel electrophoresis, and quantitative TdT assay. Results:Apoptosis was detected only in leukemic lymphocytes with P2z receptors. By using the quantitative assay, ATP-inducing DNA strand breaks were found to occur specifically for BzATP, ATP and 2MeSATP, but not for ATP-γS and other nucleosides.

将表达P2z受体［P2z］与不含P2z受体［P2z］的两组CLL细胞分别同1.0mmol/L三磷酸腺苷体外培养8小时，以电镜、DNA凝胶电泳和定量DNA 3′端TdT法检测细胞凋亡；并对ATP、苯甲酰苯甲酸ATP、2-甲基硫ATP(2MeSATP)、γ-硫代ATP及其它核苷的诱导效应和氧化型ATP、1-［N，O-二(5-异喹啉碘酰基)N-甲基-L-酪氨酰］-4-苯哌嗪KN-62）的抑制效应做定量研究。

Interleukin-2(IL-2) activity by thymocyte (C57BL/6-8 mice) proliferation assay and fibroblast(L929 fibroblasts) cytotoxicity assay has been found in supernatant of mitogen-stimulated splenocyte cultures of grass carp Ctenophaxyngodon idellus, Chinese soft-shelled turtle Trionyx sinensis.

摘 要：用刀豆蛋白A刺激诱导草鱼和中华鳖脾细胞，收集细胞培养上清液，用小鼠胸腺细胞增殖试验和对小鼠L929细胞系杀伤试验检测上清液中白细胞介素-2(简称IL-2)活性。

Methods 40 serum samples of high, middle and low bilirubin concentration were determined by vanadic acid oxidation assay and compaired with diazo method. The precision, sensitivity, stability and interference of vanadic acid oxidation assay were analysed.

收集40例高、中、低浓度的血清样本进行钒酸氧化法与重氮法的比对，分析钒酸氧化法的精密度、灵敏度、稳定性并做干扰实验。

When vitamin C concentration was ≤0.5g/L, both vanadic acid oxidation assay and diazo method bad no interference. When triglyceride was ≤6 mmol/L, the vanadic acid oxidation assay had no interference and the diazo method had significant interference.

维生素C≤0.5 g/L时对钒酸氧化法和重氮法均无干扰，三酰甘油≤6 mmol/L时对钒酸氧化法无干扰，对重氮法有明显干扰。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

Different object functions are used to accomplish the optimum assay of the North mainline. The TGNET software has been used to modeling and calibration assay of several mainlines. It has also been used to accomplish the hydraulic attestation of the optimum calculation results.

针对北干线等管线，以不同目标函数进行优化分析；利用TGNET软件对几条干线进行模拟及能力标定分析，并对优化计算结果进行水力验证。

The oil yield of six types of Huadian oil shales were measured on a Fischer assay retort,and the factors which effected the results of Fischer assay of oil shale were investigated,such as heating rate,particle size,final retorting temperature and retorting persistent time.

结果表明，页岩油的产率随着挥发分含量的增大而增加，在一定范围内提高升温速率，页岩油产率增大，但升温速率过快，反而使页岩油产率降低；终温在500℃~510℃，持温时间20 min的条件下，产油率最高，继续增加干馏终温和持续时间对产油率影响不大；同时适宜的粒度可以适当提高页岩油的产率。

Compared with the standard flask assay of Scenedesmus algae, microplate absorbance assay has some advantages such as fast easy-operating, requiring low sample volumes, easy to perform more parallel tests of toxicity and so on.

对比标准锥形瓶栅藻毒性试验，微板吸光法具有测试简便快速，所需样品体积少，便于多次平行毒性测试等优点。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。