查询词典 assay

Micrococcal lysis assay of the milk samples showed that the cDNA was efficiently expressed in the mammary glands with similar expression levels ranging from 60 to 87 mg/L. Different tissues of each vector-injected mouse were assayed for transcription of hLYZ mRNA by dot-blotting and for expression of hLYZ activity by Micrococcal lysis assay. The results showed that expression of the three recombinant vectors was mainly restricted to mammary glands with some degrees of ectopic expression in spleen, intestines and/or kidney.

注射后72 h采集乳样，经微球菌溶解实验证明，3组小鼠乳汁中hLYZ的表达量分别为87、69、60 mg/L；从每组小鼠的12种组织提取总RNA，经Dot blotting检测证明，三种重组载体除在乳腺中表达外，p205C3LYZ还在小鼠的脾和肠、pBJLYZ在脾、pBCLYZ在脾和肾中具有一定的异位表达；在有hLYZ mRNA转录的上述脏器中用微球菌溶解试验均可检测到hLYZ活性。

In addition, the sensitivity of this method was close to that using traditional microtitration plates as carriers. However, the assay was much faster (the assay time decreased from 100—120 to 30 min).

此外，灵敏度与用传统微孔板做载体相似，但测定时间更快（从100-120分钟减少到30分钟）。

SOD activity in RBC was detected by rapid catechol assay with microvolume of finger blood; serum LPO level was determined by TBA fluorescence colorimetric assay; plasma cAMP and cGMP concentrations were measured b...

超氧化物歧化酶是防御超作者单位：210042南京，中国医学科学院、中国协和医科大学皮肤病研究所；南京铁道医学院附属医院

Methods Pharynx nasalis secretion of 30 patients with acute respiratory tract infection was tested via throat swab and nasal catheter by means of gold immunochromatography assay and immunofluorescence assay.

采用咽拭子和负压吸引鼻导管两种方法取咽部分泌物，分别用GICA法、免疫荧光法检测30例急性呼吸道感染患者的咽部分泌物中的流感抗原。

Another method that has been proposed to identify S.aureus from nonclinical sources employs an immunoenzymatic assay using a monoclonal antibody assay using a monoclonal antibody prepared against S.aureus endo-β-acetyl-glucosaminidase, an enzyme produced by all isolates of this species.

另一种方法，提出了确定非临床源金黄色葡萄球菌从员工的免疫酶检测用单克隆抗体检测用单克隆抗体制备对金黄色葡萄球菌内切β-乙酰氨基葡萄糖苷酶，这种酶产生的所有分离这一物种。

In vitro\% peritrophic membrane assay showed that all three truncated enhancin lost their mucin degrading ability, while a full length recombinant enhancin was active in the assay.

通过体外降解围食膜的方法检测这些部分缺失的增强蛋白的活性，结果证实这三种蛋白均失去了增强蛋白的降解围食膜粘蛋白的活性。

Methods: We evaluated the precommercial highly sensitive cardiac troponin T assay from Roche Diagnostics and the Architect cardiac troponin I (cTnI-Architect) assay from Abbott Diagnostics by testing samples from a reference population of 546 individuals and a cohort of 85 marathon runners.

通过测定处于参考范围的546人和85位马拉松运动员的样品，我们评估了罗氏公司上市前的高敏心肌肌钙蛋白T和雅培公司开发的建筑师心肌肌钙蛋白I（ cTnI-Architect）。

The chromatin unfolding assay showed that ,like the wild-type transactivation domain, two variants that represent benign polymorphisms did not induce chromatin unfolding or only induced subtle change. Contrary to the behaviors of the wild type and two benign variants, four cancer-predisposing mutations in the transactivation domain superactivate the chromatin unfolding. The results suggest that the chromatin unfolding assay can aid in the characterization of deleterious mutations in the C-terminal transactivation domain of BRCA1 and may provide more reliable presymptomatic risk assessment.

对这些重组质粒的染色质伸展活性检测表明，野生型pwt和两种良性多态性突变体不具有染色质伸展活性或只有极微弱的染色质伸展活性，而其他4种乳腺癌易感突变体均具有过强的染色质伸展活性，提示利用染色质伸展技术可预测BRCA1转录激活区基因型与乳腺癌发生风险的表现型的关系。

Cell cycle analysis was performed using flow cytometry and proliferous index was calculated.(9) Drug sensitivity assay was performed using MTT assay.

8借助流式细胞仪对基因转染细胞及其对照细胞进行细胞周期分析，并计算细胞的增殖指数(proliferous index，PI)。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。