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Interleukin-2(IL-2) activity by thymocyte (C57BL/6-8 mice) proliferation assay and fibroblast(L929 fibroblasts) cytotoxicity assay has been found in supernatant of mitogen-stimulated splenocyte cultures of grass carp Ctenophaxyngodon idellus, Chinese soft-shelled turtle Trionyx sinensis.

摘 要：用刀豆蛋白A刺激诱导草鱼和中华鳖脾细胞，收集细胞培养上清液，用小鼠胸腺细胞增殖试验和对小鼠L929细胞系杀伤试验检测上清液中白细胞介素-2(简称IL-2)活性。

Methods 40 serum samples of high, middle and low bilirubin concentration were determined by vanadic acid oxidation assay and compaired with diazo method. The precision, sensitivity, stability and interference of vanadic acid oxidation assay were analysed.

收集40例高、中、低浓度的血清样本进行钒酸氧化法与重氮法的比对，分析钒酸氧化法的精密度、灵敏度、稳定性并做干扰实验。

When vitamin C concentration was ≤0.5g/L, both vanadic acid oxidation assay and diazo method bad no interference. When triglyceride was ≤6 mmol/L, the vanadic acid oxidation assay had no interference and the diazo method had significant interference.

维生素C≤0.5 g/L时对钒酸氧化法和重氮法均无干扰，三酰甘油≤6 mmol/L时对钒酸氧化法无干扰，对重氮法有明显干扰。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

Different object functions are used to accomplish the optimum assay of the North mainline. The TGNET software has been used to modeling and calibration assay of several mainlines. It has also been used to accomplish the hydraulic attestation of the optimum calculation results.

针对北干线等管线，以不同目标函数进行优化分析；利用TGNET软件对几条干线进行模拟及能力标定分析，并对优化计算结果进行水力验证。

The oil yield of six types of Huadian oil shales were measured on a Fischer assay retort,and the factors which effected the results of Fischer assay of oil shale were investigated,such as heating rate,particle size,final retorting temperature and retorting persistent time.

结果表明，页岩油的产率随着挥发分含量的增大而增加，在一定范围内提高升温速率，页岩油产率增大，但升温速率过快，反而使页岩油产率降低；终温在500℃~510℃，持温时间20 min的条件下，产油率最高，继续增加干馏终温和持续时间对产油率影响不大；同时适宜的粒度可以适当提高页岩油的产率。

Compared with the standard flask assay of Scenedesmus algae, microplate absorbance assay has some advantages such as fast easy-operating, requiring low sample volumes, easy to perform more parallel tests of toxicity and so on.

对比标准锥形瓶栅藻毒性试验，微板吸光法具有测试简便快速，所需样品体积少，便于多次平行毒性测试等优点。

Most of water-soluble cyanine dyes have -SO3H and –COOH in their molecules, which will influence the biomolecules' functional characters. A new kind of water-soluble cyanine dyes with several cations in molecules were designed and synthesized, preparation conditions of intermediate and dyes were researched and optimized, the yield of dye is 25%. The novel kind of cyanine dye are water-soluble , spectrum characters was researched , all the dyes were charactered by 1H NMR and TOF-MS, spectrum in different solvent of novel dyes were focused on, one of the kind dyes show higher fluorescence quantum yield than the result reported. Because of several cations in the structure, it is promising when used in DNA assay and other bio-assay field.

大多数水溶性的菁类荧光染料分子结构中都带有-SO3H和-COOH等基团，过多的阴离子的引入容易造成对生物分子的功能属性的影响，本论文设计并合成了分子结构中带有多个正电荷的水溶性的一类新型菁染料，考察并且研究了中间体和染料的合成条件，最终的目标产物产率达到25％，染料通过了质谱和核磁的表征，新型的染料具有良好的水溶性，研究了新染料在多种溶剂的的光谱性质，其中一种染料的在水中的荧光量子产率比文献报道得到了提高，由于富集了正电荷，这类菁染料在DNA标记等生物中有一定应用前景。

RESULTS MTT assay showed that hydroquinone inhibited the growth of cells in a concentration-dependant manner and the survival number of XRCC1-deficient cell was less than that of the two control groups. Comet assay revealed that different concentrations of hydroquinone caused more severe DNA damage in XRCC1-deficient cell line than in control cells and there were no significant difference in the two control groups.

结果 MTT结果显示，不同浓度10~100μmolL^(-1氢醌作用的XRCC1缺陷细胞，490 nm波长处吸光度值低于对照组细胞，提示缺陷细胞的细胞存活率比正常细胞低；彗星实验结果显示，不同浓度的氢醌对XRCC1缺陷细胞DNA损伤比对照组细胞更严重，而2个对照组细胞之间没有明显差异。

Methods The Biotin-Strept -Avidin enzyme linked immunosorbent assay, spectrophoto-metric assay were used to measure the plasma levels of CT-1 and sICAM-1 in 56 patients with hypertension before and after treatment of Perinopril or Benidipine and 30 normal controls.

应用生物素－链霉亲和素酶联免疫吸附法对56例高血压患者应用培哚普利和贝尼地平治疗前后及30名正常健康人，测定血浆CT-1水平和血浆sICAM-1水平。

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？