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Methods: Growth inhibition of dl1520 on CNE-2 cells was examined with MTT colorimetric assay and cytopathic effect assay.

用MTT法和细胞病变试验测量d11520在体外对CNE-2细胞的抑制和杀伤作用。

The confirmative tests of weak-reactive samples included neutralization assay for specific antigen and immune-blotting or immuno-fluorescence antibody assay for specific antibody.

确认方法可分为特异抗原确认的中和试验和特异抗体确认的免疫印迹或荧光抗体试验。

GdCl3(10mg/kg) or the same volume of NS was continually injected of vein at 48h and 24h before LPS(5mg/kg) was injected in the male kunming species mouse. Then took out of liver or isolated KCs 30 minute after LPS was injected and assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of liver or KCs in vivo.Ⅱ. Isolated and cultured KCs of mouse, 1 h pretreatment with GdCl3; Culture medium with LPS(100ng/ml) was added and continuatively incubated 30 minute. Assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of KCs in vitro and detection effect of GdCl3 on its phagocytosis function in respectively.

Ⅰ 雄性昆明种小鼠在注射LPS（5mg/kg）前48h、24h，分别静脉注射GdCl3（10mg/kg ）或等量的生理盐水，于LPS或生理盐水注射后30min，分别取出肝脏或分离KCs，检测肝脏或KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平；Ⅱ体外培养小鼠KCs经GdCl3（100μM）预处理1h，加入含LPS（100ng/ml）的DMEM培养基继续孵育30min，分别检测体外培养KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平和GdCl3对其吞噬、分泌功能的影响。

Methods Electrophoretic mobility shift assay and transient transfection assay were used in this study.

采用电泳迁移改变分析和荧光素酶报告基因瞬时转染分析。

Genesee strives to establish its core product pipelines in antibodies and recombinant antigens to meet the needs of a wide variety of diagnostic techniques, such as latex agglutination assay, capture ELISA, double antigen ELISA, double antigen-mediated lateral flow immuno-chromatograph, chemoluminescence, multiplex microsphere assay.

公司致力于抗体和重组蛋白两种核心原材料产品的开发，以满足各种诊断技术的需求，如乳胶凝集试验法，间接法ELISA，双抗原ELISA，双抗原介导的胶体金快速诊断，化学发光法，蛋白液体晶片多重诊断技术。

Methods The virus titer in culture fluid was measured by enzyme-linked immunosorbent assay.The HTNV-antigen in MsC was detected by indirected immunofluoresence assay and enzyme immunoassay.The shape and ultrastructure of infected MsC were observed by light microscope and transmission electron microscopy respectively.

采用免疫标记技术和电镜技术观察HTNV-A9株对人胚肾小球系膜细胞的感染情况和病毒唑、磷甲酸钠对病毒感染的抑制作用。

Trypan blue exclusion assay and cell growth curve were used to observe proliferation. Fluctuation of CD11b, reactive oxygen species and NF-кB were detected using flow cytometry. Cell apoptosis was observed using DNA laddering assay.

选择NB4细胞，加入ATRA和不同剂量的As2O3，观察细胞生长曲线，流式细胞仪检测CD11b、ROS、NF-кB的变化，DNA片段梯度观察细胞凋亡。

Micrococcal lysis assay of the milk samples showed that the cDNA was efficiently expressed in the mammary glands with similar expression levels ranging from 60 to 87 mg/L. Different tissues of each vector-injected mouse were assayed for transcription of hLYZ mRNA by dot-blotting and for expression of hLYZ activity by Micrococcal lysis assay. The results showed that expression of the three recombinant vectors was mainly restricted to mammary glands with some degrees of ectopic expression in spleen, intestines and/or kidney.

注射后72 h采集乳样，经微球菌溶解实验证明，3组小鼠乳汁中hLYZ的表达量分别为87、69、60 mg/L；从每组小鼠的12种组织提取总RNA，经Dot blotting检测证明，三种重组载体除在乳腺中表达外，p205C3LYZ还在小鼠的脾和肠、pBJLYZ在脾、pBCLYZ在脾和肾中具有一定的异位表达；在有hLYZ mRNA转录的上述脏器中用微球菌溶解试验均可检测到hLYZ活性。

In addition, the sensitivity of this method was close to that using traditional microtitration plates as carriers. However, the assay was much faster (the assay time decreased from 100—120 to 30 min).

此外，灵敏度与用传统微孔板做载体相似，但测定时间更快（从100-120分钟减少到30分钟）。

SOD activity in RBC was detected by rapid catechol assay with microvolume of finger blood; serum LPO level was determined by TBA fluorescence colorimetric assay; plasma cAMP and cGMP concentrations were measured b...

超氧化物歧化酶是防御超作者单位：210042南京，中国医学科学院、中国协和医科大学皮肤病研究所；南京铁道医学院附属医院

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？