查询词典 assay

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

objective to study the efficacy of the tr/patoc 1 slide agglutination assay for vaccination new pentavalent whole cell vaccine for leptospirosis.methods using tr/patoc 1 slide agglutination assay to detect the searial of serum specimens of 100 healthy people after vaccination for leptospirosis and comparing with the microsopic agglutination test.results both basic immunizaton and 20 days after the enhanced immunization,their positive seroconversion rates were higher than 90% in tr/patoc 1 slide agglutination assay,they were not signficantly different between slide agglutination assay and mat,the antibody titer was greatly lower in 90 days after basic immunization.conclusion the tr/patoc 1 slide agglutionation assay was one of useful assays in evaluation the lately immune efficency of leptospira vaccination.

目的 采用tr/patoc 1玻片凝集试验评价钩端螺旋体疫苗免疫效果及应有价值。方法采用tr/patoc 1玻片凝集试验检测100名健康人钩端螺旋体疫苗免疫后的血清抗体变化，并与显微镜凝集试验比较。结果无论是基础免疫还是加强免疫20？d后，玻凝试验抗体阳性率高达90%以上，与标准的显微镜凝集试验比较差异无统计学意义，玻片凝集试验检测的抗体持续时间较短，90？d后阳性率已大幅度降低，表明sat检测的抗体是早期抗体。结论 tr/patoc 1玻片凝集试验操作简易，可以作为基层卫生机构监测钩端螺旋体疫苗近期免疫效果的方法之一。

Methods By using Vero cell as the host cells and with acyclovir as the positive control, anti-HSV-1 activity of the extract was observed and determined with cytopathic effect assay and plaque reduction assay. Initial studies on antiviral mechanism included three aspects: virucidal assay, attachment assay and penetration assay.

以Vero细胞为宿主细胞，阿昔洛韦为阳性对照药物，通过观察细胞病变效应与空斑减数实验测定川楝子提取物抗HSV-1活性，计算其IC50与治疗指数，并从药物对病毒的直接灭活作用、对病毒吸附的影响及对病毒穿膜的影响三个方面初探川楝子提取物抗HSV-1活性的机理。

Chemical methods of qualitative assay and quantitative assay to Chufa functional component According to chemical characteristics reactivity of Chufa functional component, chemical method of qualitative assay and quantitative assay to Chufa functional component were built.

功能因子的定性定量化学分析针对该功能因子的化学特征反应，实验选用硫酸显色法、可见光比色法、吸光度测定法和沉淀法对功能因子进行定性、定量分析。

After mass chemotherapy,ScAg assay in case screen for selective chemotherapy was better than that of ScAb assay and stool examination.ScAg assay was also practice in evaluation of control effect,disease surveillance in children,and to confirm repeat treatment cases.In short,ScAg assay applied for case screen would play an important role in control program.

结论检测ScAg用于全民化疗后选择性化疗的筛查方法优于ScAb及粪便检查，适用于防治效果考核，儿童感染的监测及确定重复治疗对象，经多年应用ScAg筛查血吸虫病对达到传播控制起到重要作用。

Methods compare with sysmex-r3500 analyzer、coulter maxm analyzer、miller ocularto determine 130 ordinary samples.results f=3.87,p.05,the difference is conspicuous.each"r"is 0.7331,0.7231,0.5011,each other are beeline correlation.about sysmex-r3500 analyzer,the inter-assay cv of quality control cv=2.95%,the sample is cv=4.42%;about couter maxm analyzer,the intra-assay cv is 5.70%,intra-assay cv is 17.02%;about miller ocular,the inter-assay cv is 26.15%.conclusion sysmex-r3500 analyzer is good at repeatability,convenience,quickness,so it is adapt to clinical batch samples quickly determine.

分别用sysmex-r3500分析仪，coulter maxm分析仪以及国际血液标准化委员会所推荐的miller窥盘法进行比较，对130份正常人标本进行网织红细胞测定。结果：f=3.87，p.05，差异有显著性。其两两之间r值分别是0.7331，0.7231，0.5011，两两之间直线相关。sysmex-r3500分析仪的质控物批内平均cv=2.95%，标本批内平均cv=4.42%，批间平均cv=3.42%；coulter maxm分析仪批内平均cv=5.70%，批间平均cv=17.02%；miller窥盘法批内平均cv=26.15%。结论：sysmex-r3500分析仪具有重复性好，简便，快捷的特点，尤其适合临床大批标本的快速测定。网织红细胞 miller；窥盘法；仪器法

About SYSMEX-R3500 analyzer,the inter-assay CV of quality control CV=2.95%,the sample is CV=4.42%;About COUTER MAXM analyzer,the intra-assay CV is 5.70%,intra-assay CV is 17.02%;About Miller ocular,the inter-assay CV is 26.15%.

SYSMEX-R3500分析仪的质控物批内平均CV=2.95%，标本批内平均CV=4.42%，批间平均CV=3.42%；COULTER MAXM分析仪批内平均CV=5.70%，批间平均CV=17.02%；Miller窥盘法批内平均CV=26.15%。

An antibody binding to IGF-IR and inhibiting the binding of IGF-I and IGF-II to IGF-IR which is characterized in that said antibody is a is ofIgG1 isotype, b shows a ratio of IC50 values of inhibition of the binding of IGF-I to IGF-IR to the inhibition of binding of IG-II to IGF-IR of 1:3 to 3:1, c inhibits for at least 80% at a concentration of 5 nM IGF-IR phospohrylation in a cellular phosphorylation assay using 3T3 cells providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0.5% heat inactivated fetal calf serum when compared to such an assay without said antibody, and d shows no IGF-IR stimulating activity measured as IGF-IR phophorylation at a concentration of 10 M in a cellular phosphorylation assay using 3T3 providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0,5% heat inactivated fetal calf serum when compared to such an assay without said antibody has improved properties in antitumor therapy.

一种已经提高了抗肿瘤治疗的特性的抗体，所述抗体结合IGF-IR并且抑制IGF-I和IGF-II与IGF-IR结合，其特征在于所述抗体a是IgG1同种型，b显示其对IGF-I与IGF-IR结合的抑制的IC 50 值与其对IGF-II与IGF-IR结合的抑制的IC 50 值的比率为1∶3-3∶1，c当与没有所述抗体的这种测定比较时，其在5nM的浓度上，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3细胞的细胞磷酸化测定中，抑制至少80％的IGF-IR磷酸化，所述3T3细胞提供400，000-600，000分子IGF-IR/细胞，和d当与没有所述抗体的这种测定相比，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3的细胞磷酸化测定中，其在10μM的浓度上没有显示作为IGF-IR磷酸化所测量的IGF-IR刺激活性，所述3T3提供400，000-600，000分子IGF-IR/细胞。

Antioxidant activity of Se-PC was investigated and compared with phycocyanin by using four different free radical scavenging assays, namely, trolox equivalent antioxidant capacity assay, DPPH free radical scavenging assay, superoxide anion scavenging assay and assay for erythrocyte hemolysis mediated by peroxyl free radicals .

通过含硒藻蓝蛋白与藻蓝蛋白对4种不同的自由基清除能力的比较，即总抗氧化能力、清除DPPH自由基、过氧化物阴离子和引发红细胞溶血的过氧化氢自由基能力测定，从而对含硒藻蓝蛋白的抗氧化活性进行评价。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。