查询词典 assay

After washed, the chromogen substrate was added and incubated for 30 minutes in the dark. Rinsed with distilled water, purple spots were observed and counted using a dissection microscope. The number of spots means the level of cytokine producted by sCTL. Analysis of ability of specific cytotoxic lymphocytes to lyse target cells: CytoTox96 Non-Radioactive Cytotoxicity Assay kit was used.

5特异性CTL裂解靶细胞能力测定：采用CytoTox96 Non-RadioactiveCytotoxicity Assay试剂盒，将经HBV core18-27短肽、重组乙肝病毒核心抗原共刺激培养13日的PBMC与HLA-A2〓的靶细胞按一定比例混合，靶细胞被裂解后，释放的乳酸脱氢酶与底物反应显色，酶标仪测定吸光度，吸光度与裂解程度成正比，按照公式计算百分裂解率。

Mutation of the gARID1 binding site in the cwp1 promoter resulted in a decrease of promoter activity and mRNA levels during vegetative growth and encystation, suggesting that the gARID1 binding sites are positive cis-acting elements of the cwp1 gene promoters in both trophozoites and encysting cells. Using cotransfection assay, we also found overexpession of gARID1 can increase luciferase activity and mRNA levels, while mutation of the gARID1 binding sites of the cwp1 promoter resulted loss of this transactivation.

我们也用萤光酵素基因接上cwp1基因启动子，如果将其cwp1启动子上的gARID1结合位置突变，也可以发现萤光酵素和讯息RNA的表现有明显的下；再用cotransfection assay，可发现gARID1会使萤光酵素和讯息RNA表现明显上升，然而如果将其cwp1启动子上的gARID1结合位置突变，这种转活化则消失。

In the 3D gel assay, the TATI stable expreesion HeLa cells can promote the pseudopodium formation, and the pseudopodium formation frequency was correspond to the TATI protein amount in TATI stable expression HeLa cells.

但在3D gel assay中，发现TATI 蛋白质在HeLa细胞株表现时会促进细胞伸出伪足，且细胞伸出伪足的比例和TATI蛋白质表现量成正比。

Nuclear Run-on Assay was used to address the contribution of transcriptioninitiation to the regulation of five histones by YSV. Actinomycin D inhibitor studies were used to detect the mRNA half-life of five histones. SLBP weremeasured by Western blot assay to identify regulation of YSV on Pre-mRNAsplicing.

应用Nuclear Run-on Assay观察YSV对人肝癌BEL-7402细胞的五种组蛋白转录效率的影响；应用放线菌素D转录抑制法观察YSV对组蛋白mRNA半衰期的影响；应用Western blot检测SLBP的表达水平观察YSV对组蛋白mRNA加工的影响。

Objective: To evaluate the clinical significance of Enzyme-linked immunosorbent assay for detection of IgG/IgM against treponema pallidum.

目的：评价梅毒螺旋体酶联免疫吸附试验(Enzyme-linked immunosorbent assay， TP-ELISA)检测梅毒螺旋体抗体IgG/IgM的临床意义。

HMGB1, IL-6 levels in plasma and ultrafiltrate were measured by enzyme linked immunosorbent assay.

采用酶联免疫吸附法(enzyme linked immunosorbent assay，ELISA)测定血浆和超滤液HMGB1、IL-6水平。

This suggested there might have cis-acting element in 3'-UTR. In order to search the possible trans-acting factor, we found RNA-protein band in H〓 cells and no band in 95D cells using 3'-UTR probe by RNA EMSA assay. At the same time, this band could be competed with the unlabled probe. There was also no band obsearved using 5'-UTR probe in both cells.

为了寻找可能与3'-UTR结合的蛋白质反式作用因子，用RNA凝胶阻滞(electrophoresis mobility shift assay，EMSA)的方法，我们在H〓细胞的胞浆蛋白质提取物与3'-UTR探针的结合反应中，发现了一条RNA-蛋白质的结合带，而在95D细胞没有结合带出现；而且这种结合带能被未标记探针竞争性抑制；5'-UTR的结合反应也没有观测到结合带的形成。

In TUNEL assay it can be observed that cystamine can reduce myocardial cell apoptosis . At the same time Western Blot analysis to prove cystamine can reduce cardiac cell apoptosis and Deth receptor and Mitochondria through theses two pathsway, NF-κB protein expression significantly increased furthermore, it promots cell survival, which can reduce cardiac hypertrophy,apoptosis.

在TUNEL assay中可以观察到胱胺可以减少心肌细胞的凋亡，同时我们也利用Western Blot证明胱胺可以减少心肌细胞的凋亡是透过Deth receptor和Mitochondria这两条路径，而这样子降低凋亡的现象很有可能与NF-kB这讯息传递的分子上升有关，因此胱胺确实具有保护心肌细胞的作用，可改善心肌细胞肥大、凋亡的情形。

For a start, via modified Boyden chamber invasion assay, we found that twenty-six Chinese medicinal herbs can suppress A549 cell invasion. In this twenty-six Chinese medicinal herbs there are six Chinese medicinal herbs without toxicity, including Davallia mariesii Moore, Phyllanthus urinaria, Prunella vulgaris, Selaginella tamariscina, Viola yedoensis and Terminalia catappa. Based on that tumor metastasis are accompanied with proteolytic degradation of the extracellular matrix and changed cell motility.

首先藉由modified Boyden chamber invasion assay，发现有26种中药具有抑制A549侵入的能力，其中有骨碎补、叶下珠、夏枯草、卷柏、紫花地丁、榄仁树的叶等6种中药不具有细胞毒性，而当癌细胞转移时常常会伴随著细胞外基质的分解及细胞移动能力的改变，在gelatin zymography与casein zymography assay中也发现到它们可以抑制A549人类肺癌细胞的MMP-2及u-PA的表现。

After analyzing the Sp1 DNA sequence within 5'-UTR and promoter region, we found one typical IRES-conserved sequence localizing within 5'-UTR of Sp1 genomic sequence. The reporter assay and polysome distribution assay revealed that Sp1 could be accumulated strongly and rapidly under hypoxia/ischemia neuron cells.

在Polysome distribution assay的结果显示，Sp1在缺糖缺氧之后转译效率有提高的情形，接著分析Sp1 5'-UTR，发现可能存在一个典型的IRES结构，利用bicistronic assay也证实了Sp1的堆积是透过IRES所导致。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了