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Then the mice were killed in different time after given the medicine wine, and the wounded limbs were got follow the articular genua, and the limbs were put in the 10% formaldehyde solution to fix them, then the histopathologic sides were done, and the change in the tissue structure were observed in light microscope.

于给药后不同时段分别处死小鼠，然后沿膝关节部位剪取小鼠的击伤腿置于10 %甲醛溶液中固定，作病理组织切片，光镜下观察组织结构形态学变化评价，评价永康宁骨康宁药酒对小鼠击伤腿肿胀有无消肿作用。

From the point of view of geobotany, with the method of correlative factors of growth of plants, a countrywide theoretic ecological division was done for the introduction of Camptotheca acuminata and three kinds of areas of ecological preferent zone, ecological adaptive zone and ecological unadaptable zone were divided. With the theory of synecology, the characteristic on environment, constitution, life form spectrum, diversity, similarity of plots of communities were researched. Applying the method of species association, discrete distribution, indensity index, fractal geometry, etc. to research relations between the species of the communities, space distribution of Camptotheca acuminata, structure of age of Camptotheca acuminata, in different communities. Using the theories of autoecology, distibutons of seeds on land and growth of seedlings in vertical and horizontal directions, biomass of organ of Camptotheca acuminata were analyzed. On the opinions of conservation biology, the external and internal factors impressing Camptotheca acuminata were analyzed to research the mechanism of the endangered species, and with using of CTM Model, the future development direction of Camptotheca acuminata in different communities were forecasted, and. Standing on the position of resources ecology, discussing the regularity of camptothein in Camptotheca acuminata of different sites and seeding of different resources, also the the derive technics of camptothecin.

本文从地植物学的角度，运用植物生长相关因子法，对我国喜树资源的生态适宜区进行了划分，理论上确定了我国的喜树的引种栽培的最适生活区域、生态适宜区域和不适宜生活区域；从群落生态学的角度，对野生喜树所在的典型群落的群落环境、群落组成、群落生活型谱、群落的物种多样性特点、群落样地间相似性进行了研究；从种群生态学的角度，运用种间联结性、离散分布模型、聚集强度指数、分析几何等分析手段，对野生喜树所在典型群落间的种间关系、喜树的空间分布格局、年龄结构等进行了分析；从个体生态学的角度，对不同年龄组的喜树的种子的散落的垂直分布、水平分布、时间分布及种子发芽率和千里重等特点、不同种源的人工喜树苗期特点、喜树的高生长和茎生长特点、喜树的器官生物量特点进行了研究；从保护生物学的角度，对野生喜树的濒危的内部因素和外部因素进行了探讨，并运用CTM模型对不同群落中野生喜树的种群趋势进行了预测；从资源生态学的角度，对我国地理环境下喜树中喜树碱含量的地理分布规律、不同种源的喜树幼苗中喜树碱含量变化规律进行了研究，并对喜树碱的提取工艺进行了探讨。

The half-life day of HCH, DDT and chlorpyrifos was 27～32, 27～33 and 22～24 respectively and the residue were detected in the substrates and fruitbodies when fungi were inoculated to the substrated, There were no cypermethrin detected in the fruitbodies, and it's half-life day in the substrate was 19～20. Carbendazim used for control germina was also detected in the substrates and fruitbodies with a half-life from 19 days to 20 days. Because of it's short half-life (2 days to 4 days), the degradation of Chlorothalonil in substrates and fruitbodie were quickly and no chlorothanonil were detected both in the substrates and fruitbodies when harves.

在接菌情况下，HCH、DDT和毒死蜱等杀虫剂在食用菌子实体和培养料中都有残留，培养料中的降解半衰期分别为27～32天、27～33天和22～24天；氯氰菊酯在培养料中的降解半衰期为19～20天，但在子实体中没有残留；多菌灵是防治食用菌病害的常用杀菌剂，在培养料和子实体中也都有残留，并且培养料中的降解半衰期为19～20天；百菌清极易降解，在培养料和子实体都没有残留，在培养料中的降解半衰期仅为2～4天。

Aiming at the two major migrating routs of wild birds in China, the ecological distribution and prevalence of avian influenza were studied based on the investigation and analysis of the occurring and relative influential factors of highly pathogenic avian influenza, serology of free birds of HAPI areas were investigation and analysis, live stocks and poultries of some areas of Heilongjiang province were investigation and analysis, the HA gene of avian influenza virus A/Goose/XJYL/10/2003HA(H_5N_1)isolated from Xinjiang goose were cloned and sequenced, NS gene of avian influenza viruses (H_5N_1) isolated in Xinjiang were also cloned and sequenced.

本文在调查、分析禽流感发生情况以及影响疫情相关因素的基础上，针对我国野鸟迁徙的2条主要路线，对我国禽流感病毒生态分布和禽流感流行现状的研究，对爆发高致病禽流感疫区的自主活动鸟类禽流感的血清学调查与分析，对黑龙江省部分地区散养畜禽流感病毒感染的血清学调查与分析，并对鹅源H5N1亚型禽流感病毒新疆分离株A／Goose／XJYL／10／2003HA基因的克隆和序列测定与分析，对H5N1亚型禽流感病毒新疆株NS基因的克隆和序列分析。

Results Among 54 WD patients, 36 were found to carry Arg778leu mutation, among whom 15 were homozygote, 21 were heterozygote, the frequency of this mutation was 47.2%. Of 46 patients with hepatic damage, 36 were found to have the mutation of Arg778Leu, among whom 34 were found to have Leu770Leu polymorphism simultaneously.

结果 54例Wilson病患者中36例存在Arg778Leu基因突变，15例为纯合型，21例为杂合型，Arg778Leu等位基因突变频率为47.2 ％；46例伴肝损害的患者中33例存在ATP7B基因外显子8的Arg778Leu突变；36例Arg778Leu突变者中34例同时伴有Leu770Leu多态性。

Rats in model control group were treated with distilled water while those in silibinin group were treated with 100 mg/kg silibinin. Meanwhile, 100 mL/L ethanol and hyperalimentation feed were given for 4 weeks. After animals were killed, TG, SOD, GSH and MDA levels were measured with liver suspension.

同时继续给予100 mL/L乙醇和高营养饲料，4周后在麻醉状态下处死大鼠，取大鼠肝块进行病理观察，测定三酰甘油、超氧化物歧化酶、还原性谷胱甘肽和丙二醛水平。

Methods: Fifty-five male adult SD rats were randomly divided into a normal control group, a saline control group and a heroin dependence group. Heroin-dependent rat models were set up by subcutaneous injection of heroin and the hypophysis were excised on the 10, 17, 24, 31, and 38 days after models were set up. Immunohistochemical SABC method, imagine analysis, and radioimmunoassay were used in the research.

成年雄性SD大鼠55只，随机分为正常对照组、盐水对照组及海洛因依赖组；皮下注射海洛因建立海洛因依赖大鼠模型，分别于模型建立的第10、17、24、31、38天取垂体组织，应用免疫组织化学SABC法、图像分析法观察大鼠垂体远侧部β-内啡肽表达。

Methods BALB/c mice (n=176) were fed with lactalbumin and its hydrolysate, the models of oral tolerance were established, and control group A, lactalbumin group, partially hydrolysed lactalbumin group and extensively hydrolysed lactalbumin group (n=44) were divided. Meanwhile, BALB/c mice (n=83) were allergized and stimulated by lactalbumin to establish the models of food allergy, and control group B (n=41) and lactalbumin allergy group (n=42) were divided.

乳清蛋白及其水解物喂养BALB/c小鼠（n=176），建立口服耐受模型，实验分为对照A组、乳清蛋白组、部分水解乳清蛋白组、深度水解乳清蛋白组（n=44）；同时，用乳清蛋白致敏、激发BALB/c小鼠（n=83）建立食物过敏模型，实验分为对照B组（n=41）和乳清蛋白致敏组（n=42）。

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法：48小时后见大量上皮细胞贴壁，细胞不规则呈圆形、椭圆形、多边形，细胞体肥大透亮，细胞核位于中央，胞膜清楚，约12-14天后细胞呈镶嵌状排列，融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

Methods: In accordance with conventional reporter gene analysis reagent preparation method, the main component Luciferase, Luciferin and ATP were serial diluted to find the optimal concentration; Reaction solution was prepared according to the optimal concentration of the main component, then ATP were serial diluted to measure their reporter gene. Then standard curve of ATP were drawed. Tumor cell lines H1299 were serial diluted and their ATP were measured by reaction solution prepared to analyse the minimum cell number.

按照常规报告基因分析试剂配制方法，将其中主要成分Luciferase和Luciferin及ATP浓度进行倍比稀释后找到最适合反应的最佳浓度值；按照最佳浓度配制反应液，将ATP浓度倍比稀释测定报告基因数值并绘制标准曲线，分析检测灵敏度；另外选取肿瘤细胞株H1299，倍比稀释后裂解细胞应用配制好的反应液进行ATP检测，分析最低检测细胞数。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。