查询词典 argu

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

On the other hand, I argu'd with my self, That this really was the way not to deliver my self, but entirely to ruin and destroy my self; for unless I was sure to kill every one that not only should be on Shore at that Time, but that should ever come on Shore afterwards, if but one of them escap'd, to tell their Country People what had happen'd, they would come over again by Thousands to revenge the Death of their Fellows, and 1 should only bring upon my self a certain Destruction, which at present I had no manner of occasion for.

另外，我也认识到，这种主动攻击野人的计划不仅不能拯救自己，反而会完全彻底地毁灭自己。因为，除非我有绝对把握杀死当时上岸的每一个人，还能杀死以后上岸的每一个人；否则，如果有一个人逃回去，把这儿发生的一切告诉他们的同胞，他们就会有成千上万的人过来报仇，我这岂不是自取灭亡吗？这是我当前绝对不应该做的事。

Argu think When It comes to..., some 2 people e m opposi te 2 revers in both e statemen but....

当说到国际体育比赛，诸如奥林匹克运动会和世界杯足球赛，有些人认为它能增进国与国之间的友善。

Hereby it s to discuss the general principle for ascertaining the client and make the detailed argu- ment when punishing the individual business,the unit s inner organ,the individual partnership and the legal corporations in the course of being disjoined,merged and revoked.

本文论证了认定当事人的一般原则，并就个体工商户、单位内部机构、个人合伙、企业法人分立、合并或撤销后实施处罚时当事人的认定作了探讨。

Of good and evil much they argu'd then, Of happiness and final misery, Passion and Apathie, and glory and shame, Vain wisdom all, and false Philosophie: [ 565 ] Yet with a pleasing sorcerie could charm Pain for a while or anguish, and excite Fallacious hope, or arm th' obdured brest With stubborn patience as with triple steel.

他们一次次在利西忘河上过渡，往返越频繁，悲伤越深切，因为过渡时，眼看忘河水近在咫尺，渴望一饮河水，即使是一小滴，也可使痛苦和忧愁一齐忘掉；但命运不允许，从中作梗，还有女魔墨杜萨，把守着渡头，用葛共的恐怖威胁他们，严格禁止他们尝试；那河水本身也飞速逃开活人的嘴，像当初飞速逃开汤达拉斯的唇吻一样。

I think people in this lifetime in a rebirth.

我觉得人在这一生里其实也在轮回。

He had to tie her hands together.

他不得不把她的双手绑在一起。