查询词典 ao

Objective : To analyze the clinical effects of treatment on distal metaphyseal Fractures with AO expert tibil Nail .Methods : From Feb. 2007 to Jan.

目的 总结运用专家型胫骨髓内钉治疗胫骨远端干骺端骨折的临床效果。

Isosorbide mononitrate is a drug used principally in the treatment of angina pectoris and acts by dilating the blood vessels ao as to reduce the blood pressure.

单硝酸异山梨酯是一种主要用于治疗心绞痛的药物，通过扩张血管来降低血压。

Moraceae are used for medicinal herbs, which were described in Shen – Nung – Pen - Ts'ao - Ching.

始载於神农本草经，其用部有桑枝、桑白皮、桑叶、桑椹。

After 48h exposure to 50mg/L ox-LDL, under the fluorescent microscope partial cells Showed typical apoptotic morphological changes such as nacarat fluorescent body shrinking and fragment of the nucleus. At the same time, the cellular cholesterol content also increased obviously, and many lipid droplets accumulated in cells.

RAW264.7细胞经50mg／L ox-LDL处理48h后，AO／EB染色显示明显的细胞凋亡形态学特征，出现核固缩、碎裂，同时细胞内胆固醇含量显著增加(由76.3μg／mg蛋白上升至368.5μg／mg蛋白)，油红O显示细胞内有大量的脂滴存在。

Apparent, both sides is contending for Yahoo partner in publicly insecurity people the voting authority on August 1, guest of analytic personage rich points out, 3 weeks of future will be Yang Zhiyuan of person of Yahoo palm door the day of the most difficult Ao, fatidical even he besides nonofficeholding have no alternative.

显然，双方都正在舆论上紧张争夺雅虎股东们在8月1日的投票权，有分析人士博客指出，未来的三周将是雅虎掌门人杨致远最难敖的日子，甚至预言他除了下台别无选择。

Ok, here ao, noy pany, my machines and my management?

（毛，来聊聊我的公司，我的设备和我的管理人员？

The auspices of Nyaya Health to science and technology, Jilin Yongxing Wood, Guangzhou Veron environmental protection, Ao Siqi wood, three flowers Danfoss, the New World communications, exhibition design companies.

主持正理生能科技、吉林永兴木业、广州贝隆环保、奥斯奇木业、三花丹佛斯、新大陆通信等企业展览展示设计。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Methods: MTT was used to determine the proliferative effects of drug on tumor cells.

MTT法检测硒化壳聚糖对细胞增殖的影响，AO/EB荧光染色用来观察药物对细胞的凋亡作用。

RESULTS: The layer of CA1 pyramidal cells in both AO groups became thinner with pyknotic nuclei than control.

结果：与对照组相比，各氯化铝组均可见海马CA1区细胞层变薄伴明显核固缩。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。