查询词典 antigenicity

Methods ①Human peptides of CYP450 enzymes and their proteinic sequence were analyzed by means of bioinformatics, and certain sequences were chosen and synthesized according to four indexes (Hydrophilicity Plot, Flexible Regions, Antigenic Index and Surface Probability Plot) after homology analysis by software Blast. The synthesized peptides were conjugated to keyhole limpet hemocyanin to increase antigenicity.

①利用生物信息学方法分析CYP1A2、CYP3A4及CYP4A11蛋白的序列，利用DNAstar软件中蛋白质的亲水性结构、柔韧区、抗原指数及表面概率结构指标选择多肽序列，应用Blast软件对所选序列进行同源性分析并合成多肽，与载体蛋白钥孔戚血蓝素偶联，免疫大耳白兔制备抗CYP1A2、3A4及4A11抗体。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

It was believedthat analysis of the proviral intermediate DNA andthe antigenicity of its structural proteins wouldsuply valuable data and method for the further re-search to EIAV on the molecular level in the future.

本文对马传贫分子特性的探索性研究为今后进一步深入研究马传贫病毒的毒力变化和免疫的分子机制提供了有价值的参考数据和分析方法。

Result A strain of Pseudomonas cepacia was detected in 1.6% compound quaternary ammonium disinfectant before application. The amount of the germ was 1.21×105cfu/ml. The strain had the similar resistance to chlorine-containing disinfectants and glutaraldehyde compared with Staphy aureus. The contaminated disinfectant could still thoroughly destroy the antigenicity of HBsAg with 60min's contact after diluted for 5-20 times.

结果 该洋葱假单胞菌对氯和戊二醛的抗力与大肠杆菌和金黄色葡萄球菌接近，污染样液除菌后1 000倍稀释对大肠杆菌和金黄色葡萄球菌仍有较好的杀菌效果，20倍稀释可完全破坏HBsAg抗原性，和抑制枯草杆菌黑色变种芽胞的生长，1 600倍稀释可抑制白色念珠菌生长，而原液则不能抑制分离菌株的生长。

As a new biomaterial scaffold, ADM has better tissue compatibility and lower antigenicity. It has been used in the field of periodontics.

作为一种新兴的皮肤黏膜移植材料，ADM拥有良好的组织相容性和极低的免疫性，现已被应用于牙周领域。

The nuclear protein encoded by N gene is consist of 422 AA residues (47kDa), Each virion contains 1258 copies that can effectively protect viral RNA from being digested by nucleotidase. The N protein presents a group specificity, it exists in various types and subtypes of VS and plays a very important role in transcript copy. Due to its characteristics of high antigenicity, the N protein can stimulate the body producing nonneutralized antibody.

它可有效的保护病毒RNA免受各种核酸酶的消化。N基因编码的N蛋白呈群特异性，为许多型和亚型所共有，有高的抗原性，刺激机体产生非中和抗体，且在转录复制中担任了重要的角色。

Collagen has many useful features such as low antigenicity, controllable biodegradation, biological absorbable properties, haemostatic properties, ability to promote cellular growth and attachment.,which make it an excellent naturally occurring biomaterial, it has a wide application in medicinal industry and clinical medicine.

胶原蛋白是一种天然存在的具有很高利用价值的生物物质，其特有的极低的抗原性、可生物降解性、生物吸收性、止血性能和促进细胞生长的功能，使得近年来在医药工业和医学临床的研究和应用日益广泛。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。