查询词典 antigen

Level of IFNγ secreted by splenocytes of immunized mice up-on antigen-specific stimulation was detected by using a cyto-pathic effect reduction assay and splenocytes proliferation of immunized mice in response to antigen restimulation was measured by MTT method.

分离免疫小鼠的脾淋巴细胞，体外用抗原再刺激，生物学方法检测IFNγ产生水平和MTT法检测淋巴细胞增殖。结果 RT-PCR检测证实pTB30m可在CHO细胞中表达。pTB30m免疫小鼠可引起较高水平的Ag85B特异性的抗体应答。

The expression product was analyzed and its antigenicity was further studied by ELISA and Wstern blot method with chimeric protein as coating antigen.we used chimeric protein as coating antigen to test anti HBs with ELISA and Wstern blot in 58 seral samples.

通过ELISA和Western blot检测表达产物的抗原性，并以表达产物为包被抗原，通过ELISA和Western blot检测58份乙肝患者血清抗 HBs。

The glycyrrhizic acid antigen is prepared through the process including following steps: dissolving glycyrrhizic acid in N, N-dimethyl formamide, dioxane or dimethyl sulfoxide, heliophobically adding N-hydroxy succinimide, reacting with dicyclohexylcarbodiimide at 0-10 deg.c for 2-5 hr; and reacting with carrier protein at normal temperature for 4-6 hr to obtain glycyrrhizic acid antigen.

本发明所提供的甘草酸抗体，是用甘草酸抗原免疫动物，然后从被免疫动物的血清中分离得到的；所述甘草酸抗原按如下过程制备：先将甘草酸溶解在N，N-二甲基甲酰胺、二氧六环或二甲基亚砜中，避光加入N-羟基琥珀酰亚胺，然后在0-10℃条件下与二环己基碳二亚胺反应2-5小时；接着与载体蛋白常温反应4-6小时，得到甘草酸抗原。

Prior studies have showed that pigs can be rendered immune to Ascaris suum infection by immunisation with crude antigen such as third stage larvae, and ES. Recently, some Escherichia coli-expressed recombinant antigen from A.

研究表明，猪蛔虫三期幼虫、分泌排泄物等天然抗原对宿主能提供有效的免疫保护，一些猪蛔虫融合大肠杆菌重组表达蛋白也可作为防控猪蛔虫病的候选疫苗。

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

His team's research, as well as other laboratories have confirmed that microbial or other material from the antigen peptide, the surface of antigen-presenting cells HLA molecules and T cell receptor recognition of mutual contacts and the formation of a trimolecular complex-mediated of the occurrence and development of rheumatoid arthritis.

他所在的课题组以及其他实验室的研究均证实，来源于微生物或其他物质的抗原多肽，抗原呈递细胞表面的HLA分子以及T细胞受体相互接触和识别形成一种三分子复合物介导了类风湿性关节炎的发生和发展。

This result indicated that the mixture of EGb761 and FMD vaccine antigen, and of 654-2 and FMD vaccine antigen could increase microvascular vasomotion extent.

表明EGb761和654-2分别与口蹄疫疫苗抗原共同作用，都能增加微血管自律运动振幅。

Squalene/Arlacel A(4:1)–MDP was used as adjuvant. First injection with 500 ug antigen was given to each animal, and three injection once two weeks with 250 ug antigen were followed. Serum Anti-ZP antibody response was evaluated by ELISA once two weeks. In eighth week after first injection, all female monkeys were encaged wi

在高等灵长类动物免疫研究证明去糖基卵透明带抗原免疫能产生抗生育作用，但不会引起卵巢功能紊乱等不良反应，糖基是导致免疫动物卵巢功能紊乱成分，为选择卵透明带抗原，用重组基因技术发展卵透明带避孕疫苗提供依据。

The expression of ZUB1 antigen had no difference after passaging,but it was significant decreased after differentiated into adipocytes and osteocytes,which indicated that the ZUB1 antigen may be an important marker of "stem" and was associated with the differentiation of MSCs.

当诱导骨髓MSCs向成骨细胞和脂肪细胞定向分化时，流式细胞检测显示ZUB1抗原表达相应减弱，提示ZUB1抗原可作为MSCs干细胞特征的标志分子，且可能参与了MSCs分化的细胞活动。

The humoral response to hepatitis B surface antigen was mildly induced and could be enhanced by reinjecting a regular dose of HBsAg antigen vaccine.

初步结果显示腺病毒载体是一种高效、安全的载体系统。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。