查询词典 antigen

Three immobilization modes of antigen to the polymers in the pH-sensitive phase separation immunoassay were investigated and compared. The results showed that the immobilization mode in the presence of N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide hydrochloride rendered the most desirable results. The immobilization efficiencies and immunological reaction activities of immobilized antigen of this mode were improved over the other two modes.

本文研究并比较了pH敏感相分离免疫分析中抗原在高分子上的三种固定化模式，结果显示由碳二亚胺偶联的固定模式效果最好，固定化效率、固定抗原的免疫反应活性较之另外两种模式均得到了提高。

ResultsThe diagnostic sensitivity of ELISA using excretory-secretory antigen was 92.5%, which was higher than that of ELISA using crude Clonorchis sinensis antigen(88.2%).

结果比较华支睾吸虫分泌-排泄抗原的诊断的敏感性高于粗抗原，分泌-排泄抗原的特异性反应明显高于粗抗原。

Human leukocyte antigen-G is a neoclassical human leukocyte class Ⅰ b antigen which was observed to be restrictively expressed by the fetal-maternal inter-fate on the extravillous cytotrophoblast.

人类白细胞抗原－G(human leukocyte antigen-G， HLA-G)属于非经典的HLA-Ⅰ类抗原分子，早期的研究发现HLA-G的表达仅局限于母胎界面的绒毛外细胞滋养层。

From the early study of the pathogenesis of pre-eclampsia people have found its association with immunological function . In 1986 Ellis et al[1] had found that the extravillous chorionic cytotrophoblast contacting with the maternal tissue expressed high levels of Human leukocyte antigen-G, a major histocompatibility tissue-specific antigen . Extravillous chorionic cytotrophoblast with high levels expression of HLA-G proteins can invade the maternal deciduas and the maternal spiral arteries by displacing the endothelial lining and destroying most of the musculoelastic tissue of these arteries .

早期研究开始，许多学者即发现其与免疫学有密切关系，至1986年Ellis等[1]发现人类白细胞抗原(human leukocyte antigen，HLA) G分子高度表达在绒毛外滋养层细胞( extravillous cytotrophoblast，EVCT)上，即浸润至子宫蜕膜层甚至达子宫浅肌层以及子宫螺旋动脉内皮层，与母血及子宫组织直接接触的滋养细胞，这种独特的组织分布提示HLA-G分子在母胎免疫耐受方面起重要作用。

Methods The SpaA was purified by electroelution from NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain C43311. The rSpaA-N was expressed in E. coli BL21 as a soluble protein by IPTG inducing,and purified with GST affinity chromatography. Mice of each group were subcutaneously immunized three times with 50μg or 100 μg of native SpaA, rSpaA-N or NaOH-extracted antigen with in complete or incomplete Freund adjuvant at 2-week intervals.

将猪丹毒丝菌C43311株SpaA-N以可溶形式表达在大肠杆菌BL21中，用GST Bind Resin纯化试剂盒纯化rSpaA-N，采用电洗脱法从猪丹毒丝菌C43311株NaOH提取抗原中纯化天然SpaA，将rSpaA-N、天然SpaA和NaOH提取抗原制成亚单位疫苗，同时设GST及生理盐水对照组，间隔2周分3次皮下免疫小鼠，第3次免疫后2周用100LD50猪丹毒丝菌C43065株进行腹腔攻毒，采用间接ELISA方法检测免疫组小鼠血清的抗体动态变化。

It is proved the sensor is fitted to use in detecting antibody-antigen reactions of these diseases. The results of detecting antibody-antigen reactions of Fasciola gegatica, ND, IBD and EDS by using the sensor basically conform to that with standard samples.

在实验研究中，我们先后应用电化学免疫传感器测试了大片形吸虫，鸡新城疫、鸡传染性法氏囊病、鸡减蛋综合征等的抗原抗体反应，结果与我们先前初步设定的判断标准基本相符。

The re-suits showed that IL level in serum of Ⅰ group goats decreased, whereas that of Ⅱ group goats increased, TNF-α levels in serum and IL-2 in supernatant with proliferation of PBL of goats increased after infection. PBL of goats was responsible for antigen 1 week after infection. Immune response of PBL of goats infected with Fasciola hepatica on the special and non-special antigen obviously increased. The results suggested that TNF-α would participate in the course of liver damage caused by Fasciola hepatica.

结果表明，山羊试验感染肝片吸虫后，Ⅰ组山羊血清IL-2水平在感染后有所下降，而Ⅱ组山羊血清IL-2水平在感染后有所升高；感染不同剂量肝片吸虫的山羊血清TNF-α水平和淋巴细胞IL-2分泌水平均有不同程度的升高，在感染后第1周淋巴细胞就有反应，并且对非特异性刺激原和特异性刺激原的应答都有显著升高。

Genesee strives to establish its core product pipelines in antibodies and recombinant antigens to meet the needs of a wide variety of diagnostic techniques, such as latex agglutination assay, capture ELISA, double antigen ELISA, double antigen-mediated lateral flow immuno-chromatograph, chemoluminescence, multiplex microsphere assay.

公司致力于抗体和重组蛋白两种核心原材料产品的开发，以满足各种诊断技术的需求，如乳胶凝集试验法，间接法ELISA，双抗原ELISA，双抗原介导的胶体金快速诊断，化学发光法，蛋白液体晶片多重诊断技术。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Restrictive changes of TCR Vp repertoire and clonal expanded T cells could be found in peripheral blood T cells from patients with T-ALL. However, the clonal expanded T cells' property (clonal expansion leukemic cells or leukemia antigen-specific expansion T cells) should be further characterized. It may play a certain role on detection of minimal residual disease and design of anti-leukemia idiotypic vaccine. The dominant utilization of TCR VjJ repertoire could be found in peripheral blood T cells from patients with B-ALL, meanwhile clonal expansive T cells were existed. It may be a feature of the host immune response for leukemia-associated antigen. The clonal expansive tendency of T cells in V021 and VP23 subfamilies was rather obvious, which may be correlated with certain malignant B-cell clone. The CDR3 sequences of monoclonal expansive T cell in T cell strains were different, which was the base of developing DNA vaccine.

T-ALL患者外周血T细胞的TCR Vβ谱系出现限制性改变，均可检测到克隆性增殖T细胞，尚需进一步鉴定其性质(肿瘤性或抗原特异性增殖)，对于研究微小残留病变检测和设计抗白血病独特型疫苗均有一定的意义。B-ALL病人外周血T细胞的TCR Vβ谱系呈现优势利用的特点，并存在克隆性增殖的T细胞，这可能是机体对白血病相关抗原产生的特异性免疫反应；Vβ21和Vβ23亚家族T细胞发生克隆增殖改变的趋向性比较明显，可能与B-ALL相关抗原刺激有关。T细胞株中检测到的肿瘤克隆其CDR3序列具有高度的特异性，是构建DNA疫苗的基础。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。