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The allergy quarantine with avian bacillustuberculosis PPD indicated that there is significantly difference between the pVAXl-hsp65,pVAX1-hsp65-10 groups and supersonic antigen of MAP group (P＜0.01).This indicates that the Hsp65 andHsp 10 does not interfere with detection of Paratuberculosis allergy.

禽结核杆菌PPD皮试结果显示，pVAX1-hsp65和pVAX1-hsp65-10免疫组与副结核分枝杆菌超声抗原免疫组差异极显著(P＜0.01)，表明Hsp65和Hsp10不干扰变态反应检测。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

Results: The proliferation of Kasumi-l cell was obviously inhibited by VPA on dose- and time-dependent manner. The IC50 was 2.33 mmol/L treated with VPA for 72 h. Incubation with VPA, the cell ratio at G0/C1 phase increased. VPA induced myeloid cell differentiation antigen CD11b to increase with a time-and dose-dependent way and caused apoptosis. Wright's staining and light microscope were used to show the morphology of partial differentiation and obvious apoptosis with 3 mmol/L VPA for 72 hours. The typical DNA ladder of apoptosis characteristic was observed with DNA gel electrophoresis.

结果：VPA能显著抑制Kasumi-1细胞的增殖，且呈剂量和时间依赖性，VPA处理Kasumi-1细胞72h时的半数抑制浓度(IC50)为2.33 mmol/L；VPA处理后，细胞周期检测G0/G1期细胞比例逐渐增高；VPA能诱导髓系分化抗原CD11b表达水平的阳性率升高，呈时间及剂量依赖性；VPA能诱导Kasumi-1细胞凋亡，Kasumi-l细胞经VPA3 mmol/L处理72h后，出现典型的凋亡细胞所具有的梯形DNA条带。

The present study suggests that Medpor and PCL have good biocompatibility, no toxin and antigen free.

Medpor和PLA具有良好的生物相容性，无毒性及免疫原性，是较好的生物材料。

In addition, the purified IgY was digested with pepsin and the fragment with specific antigen binding was produced.

另外，IgY经胃蛋白酶（pH4.0，37℃）酶解后，得到片断抗体Fab。

The surface antigen expressions of MNC were detected by flow cytometry. MNC were induced with DMEM medium, containing HGF alone, FGF4 alone, HGF+FGF4 or no growth factor, respectively. The markers of hepatic linear cells were examined before induction, and 7, 14, 21 and 28 d after induction by RT-PCR, immunocytochemistry and periodic acid-schiff in every group.

分别用肝细胞生长因子、成纤维细胞生长因子-4(FGF4)、HGF+FGF4、无生长因子4种处理因素进行诱导培养，于诱导前及诱导后的第7、14、21、28天采用RT-PCR检测AFP、白蛋白及C-met、FGFR2 mRNA的表达，免疫细胞化学法检测肝细胞抗原和CK18的表达，PAS 法进行糖原染色。

Which laid a molecular immunological foundation of developing candidate antigen of pertussis vaccine.

为研制百日咳疫苗候补抗原的相关分子免疫学研究奠定了基础。

IFN-γcomes from antigen activated T cells and NK cells, which have function on immunoregulation and effect factor in anti-virus and anti-tumor including inducing activation of cytotoxic T lymphocytes cells, NK cells and phagocyte.

IFN-γ产生于抗原激活的T细胞和NK细胞，它扮演了涉及到抗病毒和抗肿瘤功能的免疫调节角色和效应因子功能，包括诱导CTL细胞、NK细胞和吞噬细胞依赖的活性的激活。

To study the effect of antigen-pulsed dendritic cells on the proliferation,phenotype and function of cytokine-induced killers.

研究负载抗原后的树突状细胞对细胞因子诱导的杀伤细胞增殖活性、表型和功能的影响。

It is of great importance to find out the antigenic epitope of TRP-2 as an antigen in pigmental cells, hi order to set up a foundation to investigate the epitope of TRP-2, we studied on the cloning and expression of human TRP -2 code gene.

确定其抗原表位对于深入研究该抗原及临床应用具有极其重要的意义，为了给TRP—2抗原表位确定的研究打下基础，本研究克隆了人TRP—2编码基因并表达了TRP—2蛋白。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。