查询词典 antigen

The reaction betwe en antigen with antibody was carried out by one step balance method and cultured i n 4℃ for 24 h,then separated binding and free antigen by PR reagent.

用氯氨T法制备125I标记IL-8，经Sephadex G-25 纯化，抗原抗体反应采用平衡一步法，4℃培养24 h后经PR试剂分离结合和游离的标记抗原。

Identification of extensive phenotypic information to 6 pare cultures and detection of the mol% G+C ratio of the DNA to representative strains, showed that the examined bacteria belonged to a new morphovar of Acinetobacter junii, and was designated as Acinetobacter junii Morphovar Ⅰ. In addition, serotype, antibiotic sensitivity and pathogenicity of isolates were studied, the results showed that the 6 strains have the same K-antigen and O-antigen, there arc no obvious differences in sensitivity and resistance to used 37 antimicrobial agents between strains, and have strong pathogenicity to experimental stone bonder and Bastard halibut.

经对6株纯培养菌在形态特征、理化特性等方面较系统的表观分类学指征鉴定及代表菌株DNA中G+C mol%的测定，表明为琼氏不动杆菌的一个新形态型并定名为琼氏不动杆菌形态型Ⅰ(Acinetobacter junii morphovar Ⅰ)；同时，对该菌进行了血清型、对抗菌类药物的敏感性及致病作用等方面的试验，初步表明此6株菌具有同种的表面及同种的菌体抗原，对供试37种抗菌类药物在不同菌株间的敏感及耐药无明显差异，对供试石鲽及牙鲆均具有较强的致病作用。

The diversity of the modification of HLA antigen camouflaged by varied mPEGs was closely associated with the amides displayed on the surface of HLA antigen.

不同mPEG对HLA抗原修饰效果的差异与暴露在HLA抗原表面的氨基酸有关。

Methods Indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of Aspergillus fumigatus,respectively.Two different double monoclonal antibody sandwich ELISA assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of Aspergillus,Penicillium marneffei,and 5 species of Candidas.

采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心ELISA法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。

Convincing evidence indicates that matching for HLA class I cross-reactive groups rather than conventional HLA antigen matching may result in improved renal graft survival. T and B lymphocyte crossmatch before transplantation was done to determine whether there is any human leukocyte antigen antibodies to the graft.

等待肾脏移植的病人数量有限，然而要找到HLA配对很好的机率真的很低，后来有新的策略，将大量的HLA等位基因缩减为一些决定常见的HLA抗原的少量联系紧密的HLA等位基因组，称为交叉反应组。

CD154 plays an important role in the interaction between antigen-specific T lympho- cytes and antigen-presenting cells.

CD154在抗原特异的T淋巴细胞和抗原提呈细胞的相互作用中发挥非常重要的作用。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

The potency of antigens were higher than 6.0 IU/mL. The antigen is suitable immunising horse for producing rabies immunoglobulin.Keywords : Vero cells;Horse serum;Antigen

第5，8天收获病毒滴度可稳定在7.0logLD50/ mL以上，灭活抗原具良好的抗原性，用NIH法测定效价达6.0IU/ mL以上，可用作抗原生产抗狂犬病血清。

Although macrophages had been pulsedwith tumor antigen in group of cell contrast and LacZ gene contrast group,adoptiveimmunotherapy had no influence on treated murines,because macrophages could notexert their ctotoxicity and antigen presentation function until they must be activatedfirst.

此外，巨噬细胞必须活化后才能发挥其效应和抗原提呈，因为未处理组和LacZ基因转染组虽经肿瘤抗原冲击，检测指标与质量效果均无变化。

Much more prolongation ofsurvival term and improvement of survival rate,as well as appearance of a great dealof lymphocytes around pulmonary metastatic nodes and less pulmonary metastaseswere also observed in murines treated with activated、tumor antigen-pulsedmacrophages contrast to those treated with IL-2 gene-transfected macrophageswithout prior pulse of tumor antigen in vitro.

肿瘤抗原冲击的IL-2基因转染巨噬细胞治疗组小鼠期存活明显延长，肺转移结节明显减少，局部病理显示，肺肿瘤周围出现大量淋巴细胞浸润，我们推测这可能是因为抗原冲击的活化巨噬细胞除了增强对肿瘤细胞直接的杀伤效应外，还对其进行处理加工，将免疫信息传递给周围的淋巴细胞，从而引起其增殖活化，并浸润肿瘤组织中。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。