查询词典 antigen

The fused dominant peptide antigen 38 kD-ESAT6-CFP10 might serve as a serodiagnostic candidate antigen, and the double antigen sandwich ELISA using the fused antigen might be used to detect the tuberculosis and improve the specificity and sensitivity of diagnostic reagent.

该种抗原优势肽段融合抗原有望成为结核病血清学诊断的重要候选抗原，并且双抗原夹心ELISA方法有助于提高检测的特异性和敏感性。

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

Coil O85 antigen contained 8 varieties of genes, including UDP-Nacetylglucosamine-2-epimerase gene (f1). UDP-galactopyranose mutase gene, glycosyl transferase genes (f3, f5, f6, f8), O-antigen transferase gene and O-antigen polymerase gene, in which two genes, wzx and wzy and 4 pairs of primers were identified to be specific to E.

发现8个开放阅读框架并确定功能，分别为：UDP-N-乙酰葡萄糖－2-异构酶基因，吡喃型UDP-半乳糖变位酶基因，糖基转移酶基因（orf3、orf5、orf6和orf8），O-抗原转运酶基因和O-抗原聚合酶基因。

This has been discussed before and demonstrated in previous studies but for e-antigen and in the surface antigen the hepatologist considers that they are very important in inducing the immune tolerance because this kind of antigen can induce the impairment of dendritic cells and also can induce the generation of regulatory T cells.

HBeAg在诱导免疫耐受方面的作用已经被讨论过并在以前的研究中证明过，现在肝病学家认为HBsAg在诱导免疫耐受方面也有重要作用，因为这类抗原能诱导树突状细胞的损伤，并能诱导产生调节性T细胞。

The antigen trait and immunogenicity of E. ovi were analyzed using SDS-PAGE and western blotting, then the E. ovis antigen was used as a diagnostic antigen to detect 64 serum samples by ELISA.

将提取的羊附红细胞体抗原进行SDS-PAGE电泳及免疫印迹分析，并以此抗原为诊断杭原，用ELISA方法对64份羊的血清样本进行了初步检测。

Objective: To clone Em 18 antigen gene and express recombinant Em18 antigen. Serological evaluate ReEm 18 antigen for diagnostic differentiation between alveolar echinococcosis and cystic echinococcosis.

目的：克隆泡球蚴18(Em18)抗原基因，获得高效表达、有生物活性的Em18重组蛋白，对其用于两型包虫病的免疫诊断特性进行研究。

The involvement of oxidization of peroxid in the antigen-antibody reaction makes the cell membrane permeability enhanced, so the fluorescence-labeled antibody stain can infiltrate into the cell easily and contact the antigen rapidly and diffusely, and then the antigen-antibody crosslinking can be formed efficiently.

利用过氧化氢的氧化作用参与抗原抗体反应，使细胞的膜通透性增强，利于荧光标记抗体染液渗入，从而更快速、广泛地接触抗原，更有效的抗原抗体的嵌合交联。

In the method, the polyclonal antibodies are obtained by utilizing syntactic quinoxalone antibiotics multi-cluster antigen immunization; the coupling compound of the half antigen of the quinoxalone antibiotics and an egg-white protein OVA is taken as an envelope antigen; the quinoxalone antibiotics is taken as a standard; the indirect competitive enzyme-linked immune detection method for the quinoxalone antibiotics in animal food is established; a rapid and high efficient detection means is provided for the residues of the quinoxalone antibiotics in the animal food; because the polyclonal antibodies are adopted, the cost is low, and the stability and the repeatability are good; the detection limit (IC is smaller than 90) is 0.0126 ng/ml, the median inhibitory dose (IC is smaller than 50) is 0.52 ng/ml, and the detection range (IC is smaller than 20 to 80) is 0.04467 to 14 ng/ml.

本发明利用合成的喹诺酮类抗生素多簇抗原免疫得到多克隆抗体，以喹诺酮类抗生素半抗原与卵清蛋白OVA的偶联物作为包被抗原，以喹诺酮类抗生素为标准品，建立动物性食品中的喹诺酮类抗生素的间接竞争酶联免疫检测方法；为喹诺酮类抗生素在动物性食品中的残留检测提供了快速高效的检测手段，由于采用的是多克隆抗体，费用较低并且稳定性和重复性较好，检测限(IC 90 )为0.0126ng/ml、半数抑制量(IC 50 )为0.54ng/ml和检测范围(IC 20 ～IC 80 )为0.04467～14ng/ml。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Results IL-8 antigen staining was observed predominantly in the acidophilic and the epithelium cells in the nasal polyps, and immunoreactive IL-8 was distributed widely in the cell plasm of these cells, and IL-8 antigen staining was barely observed in control.

结果 鼻息肉中IL-8免疫阳性物质存在且主要分布在鼻息肉上皮层和嗜酸性粒细胞的细胞浆内；对照组中，上皮层细胞浆有少量的IL-8免疫阳性颗粒着色。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。