查询词典 antibody

The localization of neurons containing dopamine-β-hydroxylase of Tupaia belangeri brain was observed by light and electron microscopy with the HRP-PA as a labeled antibody.

用酶联葡萄球菌A蛋白作标记抗体，对树？脑含DBH神经元进行了光镜和电镜免疫组化观察。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Further improvement in antibody preparation and enrichment of protein concentration will be untaken in the near future.

许多非特异性反应的蛋白出现可能与所用的抗体纯度不够有关，因此，我们将进一步纯化此MJD多株抗体，再者，亦有可能是神经细胞所表达的MJD蛋白量太少，所以反应太弱无法被测定出来，因此，将MJD蛋白表达量增加也是未来改进的方法之一。

Drug intervention groups received either daily inhalation of budesonide, ipratropine or heparin respectively, starting on the 8th day or TGF-β1 monoclonal antibody(TB21)0.5 mg twice (6th and 19th day) via the tail veinous injection.

其它各药物干预组于制作模型第8 d起分别雾化吸入布地奈德（布地奈德组，12只）、溴化异丙托品（溴化异丙托品组，12只）和肝素（肝素组，6只）溶液。4周后检测小支气管平滑肌及胶原厚度，用免疫组化法及原位杂交法观察各生长因子在支气管肺内的表达，用放免法检测血清和BALF中细胞外基质成分Ⅲ型前胶原、层粘连蛋白及透明质酸。

Follow up of 35 additional viremic donors for up to 404 days was conducted to evaluate persistence of WNV-specific antibody.

随访另外35名的毒血症献血者达404天，以评价WNV特异性抗体的持续性。

The results showed:(1) The vitelline antibody in alimentary tract is steady.

结果表明：(1)口服的卵黄抗体在鸡消化道较稳定；(2)口服卵黄液可使感染E。

In order to study the characteristics of humoral immunity on E. tenella, specific vitelline antibody of E.

为了探讨体液免疫在鸡球虫病中的作用，通过制备柔嫩艾美尔球虫E。

Tendlla was prepared. The metabolic pattern of vitelline antibody in cecum was determined.

tenella特异性高免卵黄抗体，检测卵黄抗体在鸡盲肠中的代谢规律，对E。

With the internal and external restraining and killing experiment of E. tenella, effects of specific high immunized vitelline antibody on E.

tenella进行体内外抑制和杀灭实验，观察特异性高免卵黄抗体对E。

Anticoccidial index increased by 73.50, rate of weight increased by 39.19%, mortality decreased by 33.4% in chickens inoculated with E. tenella by administering vitelline antibody.

tenella鸡的死亡率降低33.34%，增重率和抗球虫指数分别提高39.19%和73.50；(3)特异性高免卵黄抗体对E。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。