查询词典 antibody

Results By optimization test, capture antibody and detection antibody (from PVA detection kit, Agdia, USA) were finally diluted at 1:50 (4ug/mL) and 1:25 to be used in one step CBA, respectively. A total CBA reaction time of 2 h was needed. No cross reactivity with other plant virus similar to potato virus Y, lettuce mosaic virus and tomato ring spot virus were found. Detection sensitivity for positive control sample in one-step CBA was 4 folds higher than that in normal plate double antibodies sandwich enzyme-linked immunosorbent assay.

通过实验优化检测条件，最佳捕获抗体工作浓度为4μg/mL、最佳检测抗体工作浓度为1：25倍稀释、最佳反应时间为2h；与马铃薯Y病毒、莴苣花叶病毒、番茄环斑病毒等均未出现交叉反应；阳性样品经64倍稀释后依然可检出，检测灵敏度是传统微孔板ELISA的4倍。

There were no difference in sera thyroglobulin antibody and thyroid microsomal antibody levels, age, sex and course in two groups.

两组年龄、性别和病程差异亦无显著性。

Objective: To establish the immunoassay of thyroglobulin and anti -Tg antibody, with use of Tg antiserum and Tg monoclonal antibody by immunization of highly purified Tg.

目的：提取高纯度的甲状腺球蛋白，为制备 Tg 抗血清及 Tg 单克隆抗体，建立 Tg 的检验方法及 TgAb 的测定方法做准备。

Anti-endometria antibody IgG react with uterus internal membrane disturbing the embedding and growth of the fertilized egg. The tilter of the anti-endometria antibody IgG has relationship with the early habitual abortion.

抗子宫内膜抗体IgG可与子宫内膜发生抗原抗体反应，干扰受精卵的着床和发育，可导致反复流产，而且其影响受精卵的着床和发育的程度与血清中的抗子宫内膜抗体IgG的效价有关。

We immunized the rabbit and have got high titter antibody. The antibody showed specifity with Western blot.

通过SDS-PAGE切胶回收获得了高纯度的表达产物，进而免疫家兔获得了相应抗体。

Coli genome was amplified by using PCR method. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the Mn-SOD protein expressed by pET-28a vector and purified was injected into rabbits to prepare polyclonal antibody. Western-blot was used to detect the effect of polyclonal antibody.

coli基因组中扩增Mn-SOD基因编码区，将它克隆到原核表达载体上进行大量表达和纯化，再用纯化的蛋白对新西兰大白兔进行背部多点注射，40d后取其血清，用Western-blot印迹实验测定抗体效果。

The same result can also be achieved when optimizing F8 function (Rastrigin's function). Comparing to EGA, KEGA is easy to realize and the increase of computation load can be neglected, but the performance of KEGA can be improved greatly. 2. To enhance the speed of AIA, two new methods of computing the concentration of antibody were developed. Simulation results for F8 and F15 (needle in haystack: type I) functions show that the two methods can improve the running speed of AIA more than 8. 5 times. 3. As to the drawback in defining antibody concentration, a new definition based on Euclidean distance between two antibodies and fitness is proposed. On this basis, a new AIA was constructed. The convergence performance are more better than that of AIA. 4. Combining king crossover with AAIA and DBAIA, two new AIAs, KAAIA and DKBAIA, were proposed.

仿真实验结果表明KEGA实现简单，与EGA相比，其增加的计算开销可以忽略，但性能改善十分明显； 2、针对基于信息熵的免疫算法运行速度慢的缺点，提出了二种能够加快AIA运行速度的新的抗体浓度计算方法，得到两种新的免疫算法，即加速的人工免疫算法(Accelerated Artificial Immune Algorithm，AAIA)和加速的人工免疫算法-2（Accelerated Artificial Immune Algorithm-2，AAIA-2），对测试函数F15(needle in haystack：type I)、F8函数的仿真研究表明，这两种算法的运行速度都是AIA的8.5倍以上； 3、针对AIA的浓度定义存在的缺陷，提出了一种新的基于抗体间欧氏距离和适应度的新的抗体浓度定义，并依据该定义构造了一种基于欧氏距离的人工免疫算法(Euclidean Distance-based Artificial Immune Algorithm，DBAIA)。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Diagnostic performances of anticyclic citrullinated peptides antibody and antifilaggrin antibody in Korean patients with rheumatoid arthritis.

AKA、APF、RF和抗CCP抗体在类风湿关节炎诊断和鉴别诊断中的应用。医学检验与临床 2006， 17(6)： 35-37.56 潘解萍，谢雯。抗环瓜氨酸肽抗体在类风湿关节炎早期诊断中的临床意义。

The optimum pH for labeling was between 7.5-8.0, and the optimum spiking rate of polyclonal antibody was 12μg/ml. The absorb pad, sample pad, glass fiber of ARIST and nitrocellulose membrane HF24004 of Millipore were chosen as GICA materials. The carbofuran-OVA conjugate 0.97mg/ml and sheep anti-rabbit IgG 0.48 mg/ml was dispensed with Nano-Plotter (NP 1.2) onto the NC membrane to serve as test line and control line, respectively. The colloidal gold-labeled anti-carbofuran antibody was dispensed on the glass fiber with 20μl/unit.

选用ARIST公司的吸水垫、样品垫、玻璃纤维纸和Millipore公司的HF24004硝酸纤维素膜作为试纸条材料，用三维喷点平台分别将0.97mg/ml的克百威-OVA结合物和0.48 mg/ml羊抗兔IgG间隔5mm平行定量喷涂固化于硝酸纤维素膜上作为测试线和质控线，将金标记抗克百威多克隆抗体以20μl/垫定量滴加于玻璃纤维纸上，干燥硝酸纤维素膜和玻璃纤维纸。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。