查询词典 antibody

We reconstructed the gene of human scFv and prepared scFv and scFv antibody fragments by prokaryotic expression system PET22b/BL21(DE3). After being purification by Ni-NTA column and renaturalization by urea dialyzation, the affinity and thermostability of scFv and scFv were detected.

结果表明，成功构建了scFv基因；实现了scFv和scFv在原核中的高效表达，二者的表达量均占菌体总量的35％以上，且成功的纯化了表达的重组蛋白，复性后获得了有活性的纯化重组蛋白。

In this work,atomic force microscopy,near-field scanning optical microscopy,laser scanning confocal microscopy and quantum dot labeling were used to investigate the biophysical properties(the parameters including morphology,membrane nanostructures,membrane pore, friction distribution,adhesion properties,specific interaction of antigen-antibody,distribution mode of membrane receptors and so on) of T lymphocytes stimulated by phorbol dibutyrate plus ionomycin at nanoscale.

本论文基于AFM和NSOM，结合量子点标记及激光共聚焦等技术，以人外周血T淋巴细胞为研究对象，在纳米尺度研究了活化前后以及不同活化阶段T细胞生物物理特性的变化，即细胞形态结构、膜表面纳米结构、膜孔变化、膜表面摩擦力分布、膜表面粘附特性、抗原-抗体间特异性相互作用力、膜表面受体分子分布模式等。

Human Na~+/Dicarboxylate Cotransporter 3.Fusion Protein.Antibody.Kidney.

人钠/二羧酸协同转运蛋白3，融合蛋白，抗体，肾脏

Radioimmunodetection of CEA chimeric antibody showedgood effect,but its best imaging time was late(20hr post injection)giving discommodiousness to the clinical use.

CEA嵌合抗体的放射免疫显像显示了良好的效果，但其最佳显像时间较晚(20hr)，给临床应用带来不便。

Results Non-specific antibody increased distinguishingly in each groups after drug treatment 30 days, but all tested indexes showed no anychange in 2mg/ group. Glomerular basement membrane became thick in 10mg/ group after drug treatment 90 days, but it showed normal when treatment time was shorten to 30 days. The thickness of glomerular basement membrane was very serious in in 50mg/ group and liquid containing protein was found increasing in glomerular and tubular. The thickness of glomerular basement membrane didn't ameliorate after stopping drug 30 days.

结果 给药后30天各给药组动物血清非特异性抗体明显升高。rhIL-1Ra 2mg/组其他检测指标均未见明显地改变。rhIL-1Ra 10mg/组给药后90天肾小球毛细血管基底膜明显增厚，但此剂量给药时间为30天时未见任何异常。rhIL-1Ra 50mg/组肾小球及肾小管中蛋白性液体量多，小球毛细血管基底膜增厚更为严重，且停药30天后基底膜增厚程度仍未见明显减轻或改善。

I n this research apoptosis of keratinocyte induced by interferon-γ and anti-Fas monoclonal antibody, mimicking down stream in the cascade of the pathogenesis of eczematous dermatitis, was used as an in vitro experimental model to screen drugs which can inhibit cell apoptosis.

通过比较SRB和MTT两种检测方法选用更敏感、准确的SRB方法以及用流式细胞仪同步监测SRB检测结果，建立了用SRB初步筛选抗细胞凋亡药物的方法，从而为筛选湿疹性皮炎的治疗药物奠定了实验基础。

Methods: We detected serum antibody of rattus,hare, wild boar,muntiacus reevesi ogilby, fox, dog and cattle with mCF method. SFGR were isloated from Ixodiodeas with Embryonate hens eggs and identified with mCF and PCR/RFLP.

鼠类、野兔、野猪、麂、狐狸、狗、牛血清学检测，采用微量室温补体结合法；病原分离：用鸡胚卵黄囊感染法分离SFGR，并用 mCF、PCR/RFLP技术对分离株进行鉴定。

Methods virus was isolated from in embryonated chicken eggs and the antibody of influenza virus h 9 n 2 were detected by the method of hemag-glutination inhibition.

结果 从深圳市农贸市场300只鸡只样本中分离到27株h 9 n 2 亚型流感病毒，其抗体阳性率为7%，但未能从人群中分离到h 9 n 2 亚型流感病毒。

Pascal James Imperato, dean of the graduate program in public health at the State University of New York Downstate Medical Center in New York City and a former New York city health commissioner said," The fact that they were able to do this, and very efficiently, and get such good antibody response reinforces and reconfirms that, even for the annual flu virus, we need to move away from embryonated hens' eggs ".

博士帕斯卡尔詹姆斯imperato ，院长，研究生院计划在公共卫生方面，在纽州立大学医学中心downstate在纽约市和前纽约市卫生专员说：&事实上，他们能够做到这一点，和非常有效，并取得这样的好抗体反应巩固和确，即使是每年的流感病毒，我们需要迁离蛋鸡鸡胚蛋&。

A novel method for the measurement of insulin-link growth factor I with capillary zone electrophoresis was developed. The molecular interaction between IGF-I and its polyclonal antibody was primarily studied with immunocapillary electrophoresis and the binding constant was determined. The work shows the possibility of applying CE to immuno-analysis, which could be a new clinical analytical method for diagnosis, treatment and monitoring for the endocritic and metabolic diseases.

以分子间相互作用理论为依托，以胰岛素样生长因子-Ⅰ及其抗体为研究体系，建立了用毛细管区带电泳测定IGF-I的方法，并首次用免疫毛细管电泳方法研究了IGF-I与它的多克隆抗体的相互作用，测定了它们的结合常数，进一步证实了在毛细管电泳中实施免疫分析的可能性，为内分泌及代谢性疾病等威胁人类健康的常见病的临床诊断及治疗观察提供了新的检测方法。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。