查询词典 antibody

Objective To express the N-terminal fragment of human mast cell chymase in E.coli and prepare its polyclonal antibody from mouse.

目的：在大肠杆菌中表达人肥大细胞类糜蛋白酶N端片段，并制备其鼠源性多克隆抗体。

The chymase levels were ditermined by using the method of enzyme-linked immunosorbant assay in 45 cases of chronic hepatitis. Mast cell distribution in the specimens was determined immunohistochemically by using anti-chymase antibody.

采用酶联免疫吸附实验检测45例慢性肝炎肝组织中Chymase浓度，观察不同的纤维化分期(S1-S4)，其肝组织中Chymase浓度；用免疫组织化学染色法观察Chymase单克隆抗体标记的肥大细胞分布情况。

The magnetic microbeads with 1μm diameter are synthesized by ultrafine γ-Fe2o3, cinnamene, diethylene benzene monocase combined suspendd polymerize and ultrasonic technology. The magnetic microbeads are composed of ultrafine magnetic powders, polymer and single-antibody.

采用超细r-Fe2O3粉对界面进行处理，利用苯乙烯、二乙烯基苯单体，采用悬浮聚合法和超声波技术，合成粒径为1μm左右的磁性微珠，该磁性微珠由超细磁粉、高分子材料和单抗功能基团组成。

The magnetic microbeads with 1um diameter were synthesized by ultrafined r-Fe2O3, cinnamene, diethylene benzene moncase combined suspend polymerize and ultrasonic technology. The magnetic are composed of ultrafine magnetic powders, polymer and single-antibody.

采用超细r-Fe2O3粉界表面进行处理，利用苯乙烯、二乙烯基苯单体，采用悬浮聚合法和超声波技术，可以合成粒径为1um左右的磁性微珠，该磁性微珠由超细磁粉、高分子材料和单抗功能基团组成。

Avidin-biotin complex ELISA in detecting the specific antibody in patients infected with Clonorchis sinensis was studied.

生物素一亲和素这一生物反应放大系统引入酶联免疫吸附试验建立的ABC一ELISA方法，经对一些可溶性抗原、抗体检测研究，证明敏感性和呈色反应强度均高于常规ELISA，特别有助于低水平抗原抗体检测〔卜7，。

This suggests that antibody or inhibition of cGMP-dependent protein kinase may prevent vascular hyperpermeability of severity burn shock and septic shock,and fundamentally take on a new look in treatment of shocks instead of traditional and passive clysis.

提示运用PKG抗体或抑制剂有可能防治大面积烧伤和中毒性休克时的血管通透性增加导致的血容量减少的发生，从根本上改观传统的、被动的烧伤补液抗休克方法。

Primary study showed that body weight of broilers infected with ALV-J or REV, compared with the control, was significantly depressed and antibody response to NDV vaccine was inhibited. In addition, these broilers were susceptible to bacterial agents, such as colibacillosis, causing higher lethality rate in early time.

初步研究表明：单独感染和混合感染均可引起感染鸡生长发育不良，中枢免疫器官萎缩，免疫器官指数下降，对特定疫苗免疫应答降低，同时感染鸡早期对细菌性疾病的易感性增加，死亡率升高。

In Western blotting, Ig and Ig heavy chain of the sturgeons have the same antigenicity and can be detected by antibody of rabbit anti the sturgeon Ig respectively in colloxylin membrane while light chain cannot be detected.

Western blotting的检测结果表明，3种鲟鱼Ig的重链与其Ig具有同样的抗原性，在硝酸纤维素膜上可被兔抗鲟Ig多克隆抗体所识别，而轻链的Western blotting检测结果则呈阴性。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

The total of 387287 serum samples detected in 2007, the HIV antibody masculine was confirmative gender accounted for 0.07% was 6 examples.

感染者特征中21~25岁为感染主要年龄段，以无业为主，以性行为为HIV传播的高危行为，住在城镇较多。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。