查询词典 antibody

objective: to establish a method for labeling monoclonal antibody 6c8 of b7-2 with chloramine t and to discuss the best labeling conditions.

目的：建立125i标记b7-2单抗6c8的方法，探讨其最佳标记条件。

Methods 105 cases of GTD tissues and 20 cases of normal chorion villi specimens were examined by immunohistochemical techniques using EGFR monoclonal antibody.

采用免疫组化方法，检测石蜡包埋的105例妊娠滋养细胞肿瘤组织和20例正常早孕绒毛组织EGFR蛋白表达水平。

We cooperate with Jiacang Yue's group in institute of biophysics, Chinese Academy of Sciences to use pH sensitive CdTe QDs as a proton sensor to detect proton flux driven by ATP synthesis in chromatophore. We applied the QDs-labeled chromatophores as a virus-detector to detect H9 avian influenza virus based on antibody-antigen reaction.

我们与中科院生物物理所乐家昌研究员组合作，采用所制备的pH敏感量子点作为质子探针，代替荧光染料，探测分子马达中ATP合成中所产生的质子流，检测生物大分子。

Check the chromogen / substrate solution, the simplest test method is to mark a drop of antibody enzyme substrate added to the preparation of a good solution, if desired substrate color change occurs, you can rule out the substrate factors.

检查色原/底物溶液，最简单的检测方法是将一滴标记有酶的抗体加入到制备好的底物溶液中，如果底物发生预期的颜色变化，则可排除底物的因素。

Our laboratory also found a gene like Drosophila intersex in the testis of Cyprinus carpio. According to the significant function played by Drosophila ix in sex-determination, we are interested in the function of this gene in carp. To study the functions of cIX further, I cloned the full-length cDNA of cIX. The recombinant protein is produced and purified to serve as the antigen to induce antibody.

本实验室同样也在鲤鱼的睪丸中发现了类似Drosophila intersex的基因，鉴於intersex在果蝇中所扮演性别决定的重要功能，对於此基因在鲤鱼中所担任的功能而感到兴趣，为了进一步探讨cIX的功能，本论文完成cIX全长cDNA，设计并建构cIX的重组DNA，再利用纯化之重组蛋白诱导抗体。

It's good method to authenticate staphylococci coagulase test by the slidex staph?kit method and the tube method with highly sensitivity and specificity .Otherwise ,the specificity of the slide agglutination test method is lower than the others ,perhaps it caused by staphylococci antibody in human plasm.

乳胶血凝法和试管法敏感性和特异性较高，是鉴定葡萄球菌凝固酶试验的好方法，玻片法的特异性较差，可能是由于人血浆中产生的葡萄球菌抗体所致。

Mthods: The serum levels of IL-15 were measured by a double-antibody sandwich enzyme-linked immunosorbent assay in 47 severe hepatitis B patients and 20 healthy subjects. Parameters of liver function including alanine aminotransferase and total bilirubin were detected by automatic biochemistry analyzer, meanwhile prothrombin activity were measured by automatic coagulator. In the same time, the hepatitis A, B, C, D and E virus markers were examined in all patients.

方法采用双抗体夹心酶联免疫吸附试验检测47例重型乙型肝炎患者和20例体检健康者血清IL-15的水平，并通过全自动生化仪检测血清丙氨酸转氨酶、总胆红素，全自动血液凝固仪检测凝血酶原活动度，同时采用ELISA方法检测甲、乙、丙、丁、戊型肝炎病原学。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

The freezing slices of brain tissue were made, which different locations were collected from the brains of 12 catties. Then using the antiserum as the first antiboty and the monoclonal antibody 4C11 as the collator, the slices digested or undigested by protease K were dyed by ABC dyeing. The result was used to determined the location of PrP in cattle brain.

以12健康头黄牛脑组织为研究对象，采取脑组织的不同部位制成冰冻切片，以自制抗血清为一抗和单抗4C11为参照，应用ABC染色法对蛋白酶K处理的和未作处理的切片染色，检测牛脑组织中朊蛋白的分布。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。