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Antihuman DR5 monoclonal antibody(mDRA6) and DDP could synergistic induce apoptosis of HL60 cells.

抗DR5单抗——mDRA6与DDP对HL60细胞具有强大的协同杀伤作用。

Abstract］ Objective:To study the synergistic induction of apoptosis by the combination of antihuman DR5(death receptor 5) monoclonal antibody(mDRA6) and cisplatin on HL60 cells.

目的：观察抗死亡受体5(Death receptor 5，DR5)单克隆抗体——mDRA6与顺铂对HL60细胞的协同杀伤作用。

METHODS: HUVECs were identified by microscopic observation of morphology and immunoflourescence staining with a monoclonal mouse antihuman vWF antibody.

采用胰蛋白酶消化法体外分离健康人脐静脉内皮细胞，通过光镜下形态观察和抗vWF免疫荧光染色对培养细胞进行鉴定。

Abstract］ AIM: To prepare iodine125 labeled antihuman CD40 monoclonal antibody 5H6 (125I5H6) and investigate the binding properties of 125I5H6 to HO8910 cells in vitro.

目的：研究抗人CD40单克隆抗体5H6的125I标记及与卵巢癌细胞株HO8910体外结合的生物学特性。

The immunohistochemistric staining technique and image Analysis technique were adopted to investigate the expression of NGF and its receptor in salivary gland tissues around neoplasm,ACC and MEC with rabbit antihuman NGF TrkA and p75 polyclonal antibody.

采用免疫组织化学染色技术及图像分析技术，用兔抗人NGF、TrkA及p75多克隆抗体，对肿瘤周围涎腺组织、ACC和MEC进行神经生长因子及其受体表达研究。

Methods:To produce antihuman DR5 monoclonal antibody(mDRA6) by immunizing BALB/c mice using DR5 protein. The change of DR5 expression after DDPtreated on HL60 cells was detected by FACS. Morphologic changes of HL60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA6 and DDP on HL60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

DR5蛋白免疫BALB/c小鼠，融合筛选抗DR5杂交瘤细胞，制备抗DR5单抗——mDRA6；流式细胞术测定顺铂对HL60细胞表面DR5表达及细胞凋亡率；荧光显微镜下观察mDRA6与顺铂协同作用下HL60细胞形态变化；MTT法测定不同浓度的顺铂与mDRA6对HL60细胞存活的影响；琼脂糖凝胶电泳检测mDRA6与顺铂联合对HL60细胞DNA片段化的影响。

Methods: Seventy-eight specimens of disc herniation(classified as protrusions,, extrusions, and sequestrations) and fourteen nonherniated discs were immunostained with antihuman MMP-3 monoclonal antibody, using the avidin-biotin-peroxidase complex method.

运用免疫组织化学抗生物素蛋白—生物素—过氧化物酶复合体法，通过抗MMP3的单克隆抗体，对78例椎间盘突出症和14例非椎间盘突出症的椎间盘标本进行染色。

Patients with severe or recurrent Clostridium difficile diarrhea have poor antitoxin antibody responses.

患有重症或复发性的梭菌性腹泻患者对抗毒素性抗体的治疗反应较差，目前已推荐在这类患者中使用免疫球蛋白静脉滴注进行治疗。

It is characterized by arthralgia, myalgia, fever and serological abnormity （such as antinuclear antibody and antihistone antibodies）.

药物诱导性红斑狼疮是一种药物诱导的自身免疫病，目前已知的相关药物超过80种。

Anti-CCP1 and anti-CCP2 antibody tests were performed on 79 patients with arthropathy. The sensitivity, specificity, positive predictive value, and negative predictive value for-discriminating between rheumatoid arthritis and non-RA were calculated for both tests.

材料与方法：79名关节病变的患者同时以Anti-CCP1 和anti-CCP2 抗体做测试，并区别类风湿性关节炎与非类风湿性关节炎的敏感度、特异性、阳性预测值和阴性预测值。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。